摘要:
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
摘要:
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence of Phrixotrhix vivianii and red bioluminescence of Phrixothrix hirtus are described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.
摘要:
An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
摘要:
The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R1, R2, R3, R5, Y1 and Z1 are the same as defined in the specification.
摘要:
The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.
摘要:
An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived from Cypridina noctiluca and then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.
摘要:
A gene construct incorporating any of at least two luciferase genes which emit lights with different colors using an identical substrate such that the gene can be stably expressed in mammalian cells.
摘要:
A secretory or membrane-binding chimeric protein composed of an energy-generating protein and an energy-receiving protein linked one another wherein energy transfer can arise between the energy-generating protein and the energy-receiving protein.
摘要:
A monitor protein for measuring processing of a protein comprising a processing region which contains an amino acid residue or an amino acid residue sequence to be processed and a secretory chimeric protein which originates in the stereostructure of a protein containing at least a processing region formed by the processing and shows a change in luminescent and fluorescent properties.
摘要:
The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating a Cypridina luciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating the Cypridina luciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.