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公开(公告)号：US5604123A
公开(公告)日：1997-02-18
申请号：US260042
申请日：1994-06-15
CPC分类号： C12N9/0069 , C12Q1/6897 , C12Y113/12006
摘要： The present invention provides luciferase with an amino acid sequence shown in FIG. 1, a gene encoding the the same, a recombinant vector DNA comprising the gene ligated at a site downstream of a promoter which can be expressed in a host cell, a transformant prepared by transforming the host cell with the recombinant vector DNA and a process of producing luciferase using the transformant.
摘要翻译： 本发明提供了具有图1所示氨基酸序列的荧光素酶。 1，编码该基因的基因，包含在可以在宿主细胞中表达的启动子下游位点连接的基因的重组载体DNA，通过用重组载体DNA转化宿主细胞制备的转化体和 使用转化体产生荧光素酶。
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2.发明授权Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain 有权可见近红外光探针使用荧光素酶和有机染料之间通过糖链进行能量转移公开(公告)号：US08143013B2
公开(公告)日：2012-03-27
申请号：US12222356
申请日：2008-08-07
申请人： Yoshihiro Ohmiya , Chun Wu
发明人： Yoshihiro Ohmiya , Chun Wu
CPC分类号： A61K49/0039 , A61K49/0034 , A61K49/0036 , A61K49/0041 , A61K49/0043 , A61K49/0056 , C12N9/0069 , C12Q1/66 , C12Y113/12006
摘要： An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
摘要翻译： 本发明的目的是制备对于个体成像而言极好的对近红外光具有较低生物效应，有效发射的发光探针及其用途。 本发明提供含糖链荧光素酶衍生物，其中有机荧光染料通过糖链与荧光素酶结合。
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3.发明申请Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain 有权可见近红外光探针使用荧光素酶和有机染料之间通过糖链进行能量转移公开(公告)号：US20090047219A1
公开(公告)日：2009-02-19
申请号：US12222356
申请日：2008-08-07
申请人： Yoshihiro Ohmiya , Chun Wu
发明人： Yoshihiro Ohmiya , Chun Wu
CPC分类号： A61K49/0039 , A61K49/0034 , A61K49/0036 , A61K49/0041 , A61K49/0043 , A61K49/0056 , C12N9/0069 , C12Q1/66 , C12Y113/12006
摘要： An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
摘要翻译： 本发明的目的是制备对于个体成像而言极好的对近红外光具有较低生物效应，有效发射的发光探针及其用途。 本发明提供含糖链荧光素酶衍生物，其中有机荧光染料通过糖链与荧光素酶结合。
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公开(公告)号：US20070248967A1
公开(公告)日：2007-10-25
申请号：US11660058
申请日：2006-06-09
申请人： Satoru Ohgiya , Yuki Tochigi , Takehiko Sahara , Yoshihiro Ohmiya
发明人： Satoru Ohgiya , Yuki Tochigi , Takehiko Sahara , Yoshihiro Ohmiya
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6897 , C12N9/0069 , C12Y113/12006
摘要： It is an objective of the present invention to provide a method for convenient and highly sensitive reporter assay. A secretory luminescent enzyme is used as a reporter protein so as to evaluate the expression, functions, transcriptional activity, or transcriptional control functions of a foreign gene or a foreign DNA fragment.
摘要翻译： 本发明的目的是提供方便和高度敏感的报道分析方法。 使用分泌发光酶作为报道蛋白，以评价外源基因或外来DNA片段的表达，功能，转录活性或转录控制功能。
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