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18 条记录 (耗时 0.026 秒)

    Controlled delivery of therapeutic compounds
    6.
    发明申请
    Controlled delivery of therapeutic compounds 审中-公开
    治疗化合物的控制递送

    公开(公告)号：US20060014712A1

    公开(公告)日：2006-01-19

    申请号：US11141725

    申请日：2005-05-31

    申请人： Toomas Neuman

    发明人： Toomas Neuman

    IPC分类号： A61K48/00 ,  A61K38/17

    CPC分类号： B82Y5/00 ,  A61K38/1709 ,  A61K47/67 ,  A61K48/00

    摘要： The present invention provides compositions for the controlled delivery of compounds into cells. Entry of the compound into a cell is mediated by a cell penetrating peptide capable of translocating the compound across a cell membrane. An inhibitor of cell penetrating peptide, which activity is regulatable by action of a protease, serves to limit delivery of the compound to cells and tissues having the protease activity.

    摘要翻译： 本发明提供了用于将化合物控制递送到细胞中的组合物。 化合物进入细胞是由能够将化合物穿过细胞膜的细胞穿透肽所介导的。 细胞穿透肽的抑制剂，其活性可通过蛋白酶的作用调节，用于限制化合物递送至具有蛋白酶活性的细胞和组织。

    Transdifferentiation of epidermal basal cells into neural progenitor cells, neuronal cells and/or glial cells
    9.
    发明授权
    Transdifferentiation of epidermal basal cells into neural progenitor cells, neuronal cells and/or glial cells 失效
    将表皮基底细胞转分为神经祖细胞，神经元细胞和/或神经胶质细胞

    公开(公告)号：US06949380B1

    公开(公告)日：2005-09-27

    申请号：US09488491

    申请日：2000-01-20

    申请人： Michel F. Lévesque ,  Toomas Neuman

    发明人： Michel F. Lévesque ,  Toomas Neuman

    IPC分类号： G01N33/50 ,  A61K35/12 ,  A61K35/30 ,  A61K48/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28 ,  A61P43/00 ,  C07K14/52 ,  C12N5/079 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/02 ,  C12R1/91 ,  G01N33/15 ,  C12N5/00

    CPC分类号： C12N5/0618 ,  A61K35/12 ,  A61K48/00 ,  C12N2501/13 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/60 ,  C12N2503/02 ,  C12N2506/09 ,  C12N2510/00

    摘要： Disclosed is a method of transdifferentiating an epidermal basal cell into a cell having one or more morphological, physiological and/or immunological features of a neural progenitor, neuronal, or glial cell by culturing a proliferating epidermal basal cell population derived from the skin of a mammalian subject; exposing the epidermal basal cell(s) to an antagonist of bone morphogenetic protein (BMP), such as fetuin, noggin, chordin, gremlin, or follistatin; and growing the cell(s) in the presence of at least one antisense oligonucleotide comprising a segment of a human MSX1 gene and/or a segment of a human HES1 gene, or homologous non-human counterpart of either of these. Also disclosed is a transdifferentiated cell of epidermal origin and cell cultures derived therefrom. In addition, methods of using the inventive transdifferentiated cell(s) and cell cultures to identify a novel nerve growth factor or to screen a potential chemotherapeutic agent by detecting the presence or absence of an effect, in vitro, on a morphological, physiological and/or molecular biological property of the transdifferentiated cell(s) are described, as is a method of using the transdifferentiated cell(s) and cell cultures to screen a potential chemotherapeutic agent to treat a nervous system disorder of genetic origin. A kit useful for practicing the methods is disclosed

    摘要翻译： 公开了一种通过培养来自哺乳动物皮肤的增殖性表皮基底细胞群体将表皮基底细胞转分化成具有神经祖细胞，神经元或神经胶质细胞的一种或多种形态学，生理学和/或免疫学特征的细胞的方法 学科; 将表皮基底细胞暴露于骨形态发生蛋白（BMP）的拮抗剂，例如胎球蛋白，头蛋白，chordin，gremlin或卵泡抑素; 以及在至少一种包含人MSX1基因片段和/或人类HES1基因片段或其中任一种的同源非人对应物的反义寡核苷酸的存在下生长细胞。 还公开了表皮来源的转分化细胞和从其衍生的细胞培养物。 此外，使用本发明的转分化细胞和细胞培养物来鉴定新的神经生长因子或通过在体外检测在形态学，生理学和/或生物学上存在或不存在效应来筛选潜在的化学治疗剂的方法， 或转分化细胞的分子生物学特性，以及使用转分化细胞和细胞培养物筛选潜在的化学治疗剂以治疗遗传起源的神经系统疾病的方法。 公开了一种用于实施该方法的试剂盒

    Method for inducing DNA synthesis in neurons
    10.
    发明授权
    Method for inducing DNA synthesis in neurons 失效
    在神经元中诱导DNA合成的方法

    公开(公告)号：US06372721B1

    公开(公告)日：2002-04-16

    申请号：US09408508

    申请日：1999-09-30

    申请人： Toomas Neuman ,  Kikuo Suda ,  Howard O. Nornes

    发明人： Toomas Neuman ,  Kikuo Suda ,  Howard O. Nornes

    IPC分类号： A61K317088

    CPC分类号： C07K14/005 ,  C07K14/4738 ,  C07K14/48 ,  C12N9/0071 ,  C12N15/85 ,  C12N2710/10322 ,  C12Y114/13041 ,  C12Y114/16002

    摘要： A method is provided for inducing DNA synthesis in differentiated neurons. According to certain embodiments of the invention, a method for inducing DNA synthesis in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a differentiated neuron, and transfecting a differentiated neuron with the vector. According to certain embodiments of the invention, a method for integrating DNA encoding a desired protein in a differentiated neuron is provided that includes obtaining a vector comprising nucleic acid encoding an E2F regulator and/or an E1A regulator, wherein the vector can be used to express the nucleic acid in a neuron, obtaining DNA encoding a desired protein, and cotransfecting a differentiated neuron with the vector and the DNA encoding the desired protein such that the DNA encoding the desired protein is integrated in the differentiated neuron and the desired protein is produced.

    摘要翻译： 提供了一种诱导分化神经元DNA合成的方法。 根据本发明的某些实施方案，提供了在分化神经元中诱导DNA合成的方法，其包括获得包含编码E2F调节子和/或E1A调节子的核酸的载体，其中所述载体可用于表达所述核酸 在分化神经元中，并用载体转染分化的神经元。 根据本发明的某些实施方案，提供了一种用于整合在分化神经元中编码所需蛋白质的DNA的方法，其包括获得包含编码E2F调节剂和/或E1A调节剂的核酸的载体，其中所述载体可用于表达 神经元中的核酸，获得编码期望蛋白质的DNA，并用载体和编码所需蛋白质的DNA共转染分化的神经元，使得编码期望的蛋白质的DNA整合在分化的神经元和所需的蛋白质中。