公开(公告)号：US20050129615A1

公开(公告)日：2005-06-16

申请号：US10961580

申请日：2004-10-08

申请人： Jacek Rozga ,  Toomas Neuman

发明人： Jacek Rozga ,  Toomas Neuman

IPC分类号： A61B10/00 ,  A61K9/70 ,  A61K51/00 ,  G01N33/574

CPC分类号： A61B10/0045 ,  A61B10/0064 ,  A61K9/7023 ,  A61K49/0006 ,  A61K49/0021 ,  A61K49/0056

摘要： The invention concerns methods and compositions for the diagnosis of cancer. The invention further concerns diagnostic patches which may be contacted topically with an externally accessible tissue surface for the purpose of detecting the presence or absence of cancer.

摘要翻译： 本发明涉及用于诊断癌症的方法和组合物。 本发明还涉及可以与外部可接近的组织表面局部接触以诊断癌症的存在或不存在的诊断性贴片。

公开(公告)号：US20050054099A1

公开(公告)日：2005-03-10

申请号：US10655093

申请日：2003-09-04

申请人： Yutaka Umehara ,  Jacek Rozga ,  Achilles Demetriou

发明人： Yutaka Umehara ,  Jacek Rozga ,  Achilles Demetriou

IPC分类号： A61L27/36 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071

CPC分类号： A61L27/3804 ,  A61L27/3683 ,  A61L27/3895 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/067 ,  C12N2533/90

摘要： Described herein is a novel biological support for cells, derived from a mammalian whole organ, tissue or portion thereof. In various embodiments, a support matrix is prepared by isolating a whole organ, tissue or portion thereof, and thereafter converting it into a tissue powder. Various washing and freeze-drying techniques are employed, as well as mechanical reduction, sonication and further processes to convert the whole organ, tissue or portion thereof into a matrix suitable for supporting functional longevity and proliferation of cultured cells. It is believed that by culturing a particular type of cell in a matrix derived from the whole organ, tissue or portion thereof with which that cell is normally associated, one may achieve optimal cell support and differentiation. The whole organs, tissues and portions thereof used in connection with aspects of the present invention need not be decellularized or otherwise digested prior to processing into the matrix.

摘要翻译： 本文描述的是来自哺乳动物整个器官，组织或其部分的细胞的新型生物学支持。 在各种实施方案中，通过分离整个器官，组织或其部分，然后将其转化成组织粉末来制备支持基质。 使用各种洗涤和冷冻干燥技术，以及机械还原，超声处理和将整个器官，组织或其部分转化成适合于支持培养细胞的功能寿命和增殖的基质的进一步的方法。 相信通过在源自整个器官，组织或其与该细胞正常相关的部分的基质中培养特定类型的细胞，可以实现最佳的细胞支持和分化。 与本发明的各方面有关的整个器官，组织及其部分在加工成基质之前不需要去细胞化或以其它方式消化。

公开(公告)号：US6140123A

公开(公告)日：2000-10-31

申请号：US168366

申请日：1998-10-07

申请人： Achilles A. Demetriou ,  Andreas Kamlot ,  Jacek Rozga

发明人： Achilles A. Demetriou ,  Andreas Kamlot ,  Jacek Rozga

IPC分类号： A01N1/02 ,  A01N63/00 ,  A01N65/00 ,  C12N1/00 ,  C12N1/02 ,  C12N5/00

CPC分类号： A01N1/0226 ,  A01N1/02 ,  A01N1/0221

摘要： A method is provided for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium containing a cryopreservative such as Dimethyl Sulfoxide (DMSO) and the suspension is incubated for a period of at least ten minutes and the cells are frozen. The medium includes adenosine, a calcium channel blocker, and a cell nutrient matrix comprising a sufficient amount of cell nutrients to sustain the metabolic needs of the cells during incubation without producing detectable levels of lactate or substantially depleting the nutrient substrates to maintain viability of the harvested cells. In some embodiments, a cryopreservative is added step-wise before the cell suspension is frozen and removed step-wise after the cell suspension is thawed.

摘要翻译： 提供了一种用于从供体收获的细胞的预处理和低温保存的方法。 将细胞悬浮于含有冷冻保存剂如二甲基亚砜（DMSO）的细胞调节和冷冻保存培养基中，并将悬浮液孵育至少十分钟，并将细胞冷冻。 该培养基包括腺苷，钙通道阻滞剂和包含足够量的细胞营养物的细胞营养基质，以在孵育期间维持细胞的代谢需要，而不产生可检测水平的乳酸盐或基本上消耗营养物质以维持收获的活力 细胞。 在一些实施方案中，在冷冻细胞悬浮液之前逐步添加冷冻保存剂，并在细胞悬浮液解冻后逐步除去。

公开(公告)号：US5993406A

公开(公告)日：1999-11-30

申请号：US856522

申请日：1997-05-14

申请人： Jacek Rozga ,  Achilles A. Demetriou

发明人： Jacek Rozga ,  Achilles A. Demetriou

IPC分类号： A61L27/00 ,  A61F2/00 ,  A61F2/02 ,  A61F2/04 ,  A61L27/38 ,  A61M1/16 ,  A61M1/18 ,  A61M1/34 ,  G09B23/28 ,  A61M35/00

CPC分类号： A61L27/3813 ,  A61F2/022 ,  A61L27/3616 ,  A61L27/3679 ,  A61L27/3882 ,  A61L27/3895 ,  A61M1/1627 ,  A61M1/3475 ,  A61M1/3489 ,  G09B23/28 ,  A61L2430/22

摘要： An artificial gut (100) comprises a first hollow fiber (203) having an inner surface (215) that is lined with at least a portion of a layer of one or more of a plurality of biological components (205) that typically line a mammalian gut. Further, a second hollow fiber (201) is adjacent the first hollow fiber. The layer of one or more of the plurality of biological components can consist essentially of enterocytes. The enterocyte-lined inner surface is perfused with a feeding solution containing nutrients, and the enterocytes absorb, process, and transport the nutrients across the wall of the first hollow fiber. The nutrients eventually diffuse into the second hollow fiber. A perfusate selected from the group consisting of culture medium, blood, and plasma, can perfuse an inner surface of the second hollow fiber, whereby the perfusate is nourished by the nutrients.

公开(公告)号：US5888409A

公开(公告)日：1999-03-30

申请号：US484717

申请日：1995-06-07

申请人： Eugenio Morsiani ,  Jacek Rozga ,  Achilles A. Demetriou

发明人： Eugenio Morsiani ,  Jacek Rozga ,  Achilles A. Demetriou

IPC分类号： A61B10/00 ,  A61B10/02 ,  A61B19/00 ,  A61K35/407 ,  A61M1/00 ,  C12M1/26 ,  B01D37/00

CPC分类号： A61B10/02 ,  A61B90/00 ,  C12M47/04 ,  A61B10/0045

摘要： The invention resides in apparatus and methods for isolating and collecting cells from donor tissue for later use in medical procedures. The apparatus includes a sealed enclosure, at least one screening material inside the enclosure, an inlet port configured to conduct a fluid through the screening material, and an outlet port for receiving the fluid from the screening material and conducting the fluid out of the enclosure. A material to be screened is placed inside or conducted into the enclosure. Screened material is entrained in the fluid. The fluid carries bits of the material through the screening material and out of the enclosure for later separation from the fluid. Preferred embodiments include a series of screening materials of decreasing pore size, usually in the range from ten to one thousand micrometers in diameter. A particularly preferred embodiment includes three distinct screening materials having pore sizes of 400 micrometers, 280 micrometers, and 100 micrometers, listed in the order that the fluid passes through the screening materials. The invention is used in the collection and isolation of porcine hepatocytes for later use in medical procedures. The sealed enclosure is constructed of a series of individual segments held together by conveniently removable fasteners--bolts and wing nuts. A mechanical shaker agitates the enclosure as the fluid flows over and through the material being screened.

摘要翻译： 本发明在于用于从供体组织中分离和收集细胞以用于医疗程序的装置和方法。 该设备包括密封的外壳，外壳内的至少一个筛选材料，被配置为将流体通过筛分材料导入的入口端口和用于从筛分材料接收流体并将流体导出外壳的出口。 待筛选的材料放置在外壳内或导入外壳中。 筛选的材料被夹带在流体中。 流体携带物料的一部分通过筛分材料并排出外壳以便稍后与流体分离。 优选的实施方案包括一系列减小孔径的筛选材料，通常在10至1000微米的范围内。 特别优选的实施方案包括具有400微米，280微米和100微米孔径的三种不同的筛选材料，其顺序是流体通过筛选材料。 本发明用于猪肝细胞的收集和分离，以备后来用于医疗过程。 密封的外壳由一系列单独的段构成，通过方便的可移除的紧固件 - 螺栓和翼形螺母固定在一起。 当流体流过并穿过被筛选的材料时，机械振动筛搅动外壳。

公开(公告)号：US5688237A

公开(公告)日：1997-11-18

申请号：US434460

申请日：1995-05-04

申请人： Jacek Rozga ,  Achilles A. Demetriou

发明人： Jacek Rozga ,  Achilles A. Demetriou

IPC分类号： A61M37/00 ,  A61F2/958 ,  A61M25/00 ,  A61M31/00

CPC分类号： A61M25/007 ,  A61M25/10

摘要： An implantable catheter comprises a flexible elongated catheter body having an inflatable balloon positioned near its distal end. At least one distal delivery port is located distal of the balloon and at least one proximal delivery port is located proximal of the balloon. The balloon and the distal and proximal delivery ports are connected in fluid communication with individual lumens. The lumen connected to the balloon is connected to a balloon inflation port at the proximal end of the catheter. The lumens connected to the distal and proximal delivery ports are connected to distal and proximal injection ports. Preferably, the balloon inflation port and the distal and proximal injection ports are all built into an integrated port assembly adapted for implantation under the skin of a medical patient. In use, the distal end of the catheter is inserted and fed into a passageway such as a blood vessel until the balloon and delivery ports lie within a region of interest. The port assembly is then implanted under the patient's skin. Once in place, the balloon can be inflated to occlude the flow of fluid through the passageway, and therapeutic agents can be delivered either distal or proximal of the balloon by injection into the appropriate injection port. It is believed that the catheter is particularly suited to the delivery of hepatocytes and an anticoagulant during a course of cell therapy directed to a patient's liver.

摘要翻译： 可植入导管包括柔性细长导管主体，其具有位于其远端附近的可充气气囊。 至少一个远端输送端口位于气囊的远端，并且至少一个近端输送端口位于气囊的近侧。 气囊和远端和近端输送端口与单个流明物流体连通地连接。 连接到气囊的管腔连接到导管近端的气囊充气口。 连接到远端和近端输送口的腔连接到远端和近端注射口。 优选地，球囊充气端口和远侧和近端注射端口全部内置在适于植入在医疗患者皮肤下的集成端口组件中。 在使用中，导管的远端被插入并进入诸如血管的通道中，直到气囊和输送口位于感兴趣的区域内。 然后将端口组件植入患者的皮肤下。 一旦就位，气囊可以被充气以阻塞通过通道的流体流动，并且治疗剂可以通过注射到适当的注射口中被输送到球囊的远端或近端。 据信导管特别适合于在针对患者的肝脏的细胞治疗过程中输送肝细胞和抗凝血剂。

公开(公告)号：US06485959B1

公开(公告)日：2002-11-26

申请号：US09168349

申请日：1998-10-07

申请人： Achilles A. Demetriou ,  Andreas Kamlot ,  Jacek Rozga

发明人： Achilles A. Demetriou ,  Andreas Kamlot ,  Jacek Rozga

IPC分类号： A61K914

CPC分类号： A01N1/02 ,  A01N1/0226

摘要： Disclosed is a universally-adaptable cell preconditioning and storage medium that can be used for the effective cryopreservation of cells at temperatures less than 4° C. The aqueous medium contains adenosine, a calcium channel blocker, and a nutrient-rich matrix that has a sufficient amount of cell metabolites to sustain the metabolic needs of the harvested cells while incubating the cells for a period of at least 10 minutes, without producing detectable levels of lactate or substantially depleting the metabolic substrates of the cell.

摘要翻译： 公开了一种普遍适应性的细胞预处理和储存培养基，其可用于在低于4℃的温度下有效地冷冻保存细胞。水性介质含有腺苷，钙通道阻滞剂和富营养素基质，其具有足够的 细胞代谢物的量，以维持收获的细胞的代谢需要，同时孵育细胞至少10分钟的时间，而不产生可检测水平的乳酸盐或基本上耗尽细胞的代谢底物。

公开(公告)号：US5968356A

公开(公告)日：1999-10-19

申请号：US240731

申请日：1999-01-29

申请人： Eugenio Morsiani ,  Jacek Rozga ,  Achilles A. Demetriou

发明人： Eugenio Morsiani ,  Jacek Rozga ,  Achilles A. Demetriou

IPC分类号： A61B10/00 ,  A61B10/02 ,  A61B19/00 ,  A61K35/407 ,  A61M1/00 ,  C12M1/26 ,  B01D37/00

CPC分类号： A61B10/02 ,  A61B90/00 ,  C12M47/04 ,  A61B10/0045

摘要： The invention resides in apparatus and methods for isolating and collecting cells from donor tissue for later use in medical procedures. The apparatus includes a sealed enclosure, at least one screening material inside the enclosure, an inlet port configured to conduct a fluid through the screening material, and an outlet port for receiving the fluid from the screening material and conducting the fluid out of the enclosure. A material to be screened is placed inside or conducted into the enclosure. Screened material is entrained in the fluid. The fluid carries bits of the material through the screening material and out of the enclosure for later separation from the fluid. Preferred embodiments include a series of screening materials of decreasing pore size, usually in the range from ten to one thousand micrometers in diameter. A particularly preferred embodiment includes three distinct screening materials having pore sizes of 400 micrometers, 280 micrometers, and 100 micrometers, listed in the order that the fluid passes through the screening materials. The invention is used in the collection and isolation of porcine hepatocytes for later use in medical procedures. The sealed enclosure is constructed of a series of individual segments held together by conveniently removable fasteners--bolts and wing nuts. A mechanical shaker agitates the enclosure as the fluid flows over and through the material being screened.

摘要翻译： 本发明在于用于从供体组织中分离和收集细胞以用于医疗程序的装置和方法。 该设备包括密封的外壳，外壳内的至少一个筛选材料，被配置为将流体通过筛分材料导入的入口端口和用于从筛分材料接收流体并将流体导出外壳的出口。 待筛选的材料放置在外壳内或导入外壳中。 筛选的材料被夹带在流体中。 流体携带物料的一部分通过筛分材料并排出外壳以便稍后与流体分离。 优选的实施方案包括一系列减小孔径的筛选材料，通常在10至1000微米的范围内。 特别优选的实施方案包括具有400微米，280微米和100微米孔径的三种不同的筛选材料，其顺序是流体通过筛选材料。 本发明用于猪肝细胞的收集和分离，以备后来用于医疗过程。 密封的外壳由一系列单独的段构成，通过方便的可移除的紧固件 - 螺栓和翼形螺母固定在一起。 当流体流过并穿过被筛选的材料时，机械振动筛搅动外壳。

公开(公告)号：US20060129082A1

公开(公告)日：2006-06-15

申请号：US10524448

申请日：2003-08-11

申请人： Jacek Rozga

发明人： Jacek Rozga

IPC分类号： A61M37/00

CPC分类号： A61M1/3472 ,  A61M1/3437 ,  A61M1/3486 ,  A61M1/3681 ,  A61M2027/004 ,  A61M2202/0415

摘要： Disclosed is a method for removing from a patient's blood a specific plasma fraction containing substances within a specific molecular weight range and a plasma purification apparatus by which the method can be accomplished.

摘要翻译： 公开了一种从患者血液中除去含有特定分子量范围的物质的特定血浆部分和能够实现该方法的血浆纯化装置的方法。

公开(公告)号：US06379619B1

公开(公告)日：2002-04-30

申请号：US09294737

申请日：1999-04-19

申请人： Jacek Rozga ,  Achilles A. Demetriou

发明人： Jacek Rozga ,  Achilles A. Demetriou

IPC分类号： A61M114

CPC分类号： A61L27/3813 ,  A61F2/022 ,  A61L27/3616 ,  A61L27/3679 ,  A61L27/3882 ,  A61L27/3895 ,  A61L2430/22 ,  A61M1/1627 ,  A61M1/3475 ,  A61M1/3489 ,  G09B23/28

摘要： An artificial gut (100) comprises a first hollow fiber (203) having an inner surface (215) that is lined with at least a portion of a layer of one or more of a plurality of biological components (205) that typically line a mammalian gut. Further, a second hollow fiber (201) is adjacent the first hollow fiber. The layer of one or more of the plurality of biological components can consist essentially of enterocytes. The enterocyte-lined inner surface is perfused with a feeding solution containing nutrients, and the enterocytes absorb, process, and transport the nutrients across the wall of the first hollow fiber. The nutrients eventually diffuse into the second hollow fiber. A perfusate selected from the group consisting of culture medium, blood, and plasma, can perfuse an inner surface of the second hollow fiber, whereby the perfusate is nourished by the nutrients.

摘要翻译： 人造肠（100）包括第一中空纤维（203），其具有内表面（215），内表面（215）与多个生物组分（205）中的一种或多种的至少一部分层叠， 肠道 此外，第二中空纤维（201）与第一中空纤维相邻。 多个生物组分中的一个或多个的层可以基本上由肠细胞组成。 肠内细胞内表面用含有营养物质的喂养溶液灌注，肠细胞吸收，处理和运输营养物质穿过第一中空纤维的壁。 营养素最终扩散到第二个中空纤维中。 选自培养基，血液和血浆的灌洗液可以灌注第二中空纤维的内表面，从而由营养物滋养灌注液。