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公开(公告)号：US20130253141A1
公开(公告)日：2013-09-26
申请号：US13792832
申请日：2013-03-11
发明人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC分类号： C08F136/08 , C08F236/10 , C08F236/08
CPC分类号： C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.
摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-220 / 00。
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公开(公告)号：US20160281113A1
公开(公告)日：2016-09-29
申请号：US14987681
申请日：2016-01-04
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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公开(公告)号：US09909144B2
公开(公告)日：2018-03-06
申请号：US14987681
申请日：2016-01-04
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC分类号： C12N1/20 , C12N1/00 , C12N15/00 , C12P5/02 , C07H21/04 , C12P5/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90 , C12N15/52
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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4.发明申请ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 审中-公开用于改善微生物生产异烟肼的异丙肾上腺素变异体公开(公告)号：US20150191712A1
公开(公告)日：2015-07-09
申请号：US14543628
申请日：2014-11-17
发明人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. Mcauliffe , Andrei Miasnikov , Caroline M. Peres , Christopher L. Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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公开(公告)号：US08940849B2
公开(公告)日：2015-01-27
申请号：US13792832
申请日：2013-03-11
发明人： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC分类号： C08F297/04 , C08F136/08 , C08F236/08 , C07C11/18 , C08F2/00 , C08F36/04 , C08L9/00 , C12P5/00 , C08F236/10 , C12P5/02 , C08F4/48
CPC分类号： C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.
摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22‰。
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公开(公告)号：US20190338256A1
公开(公告)日：2019-11-07
申请号：US16348018
申请日：2017-04-24
申请人： DANISCO US INC.
摘要： Described are composition and methods relate to reducing the redox imbalance in anaerobically growing yeast with attenuated glycerol production by re-engineering the pathway for Ac-CoA biosynthesis.
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公开(公告)号：US10428354B2
公开(公告)日：2019-10-01
申请号：US16245143
申请日：2019-01-10
申请人： DANISCO US INC
摘要： A recombinant yeast cell, fermentation compositions, and methods of use thereof are provided. The recombinant yeast cell includes at least one heterologous nucleic acid encoding one or more polypeptide having phosphoketolase activity; phosphotransacetylase activity; and/or acetylating acetaldehyde dehydrogenase activity, wherein the cell does not include a heterologous modified xylose reductase gene, and wherein the cell is capable of increased biochemical end product production in a fermentation process when compared to a parent yeast cell.
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公开(公告)号：US20150044736A1
公开(公告)日：2015-02-12
申请号：US14338639
申请日：2014-07-23
申请人： Danisco US Inc.
CPC分类号： C12N9/18 , C11D3/38627 , C11D3/38681 , C12P7/6454 , C12Y301/01074
摘要： Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea, polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.
摘要翻译： 描述了与从Magnaporthe grisea克隆的真菌角质酶相关的组合物和方法，以及编码角质酶的多核苷酸及其使用方法。 组合物和方法在洗涤剂清洁组合物和合成反应中具有特别的应用。
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公开(公告)号：US20140024098A1
公开(公告)日：2014-01-23
申请号：US13946958
申请日：2013-07-19
申请人： Danisco US Inc.
IPC分类号： C12N9/08
CPC分类号： C12N9/0065 , C12P3/00 , C12Y111/01006
摘要： The invention provides methods for expression of a catalase enzyme in a Trichoderma host cell. In one embodiment, the catR gene from Aspergillus niger is expressed in Trichoderma reesei, resulting in improved yields of catalase enzyme in comparison with expression of catR in A. niger.
摘要翻译： 本发明提供了在木霉属宿主细胞中表达过氧化氢酶的方法。 在一个实施方案中，来自黑曲霉的catR基因在里氏木霉中表达，与黑曲霉中catR的表达相比，导致过氧化氢酶的产量提高。
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10.发明授权公开(公告)号：US10450575B2
公开(公告)日：2019-10-22
申请号：US14909927
申请日：2014-07-31
申请人： DANISCO US INC.
发明人： Andrei Miasnikov
IPC分类号： C12N9/22 , C12N15/67 , C12N15/63 , C12N15/85 , C12N9/12 , C12N9/16 , C12N9/30 , C12N9/42 , C12N9/48 , C12N15/80
摘要： Described are methods for increasing the expression of a transgene in eukaryotic cells by reducing RNA interference (RNAi), and variant cells produce by the method. The methods and variant cells are useful, for example, for the efficient production of therapeutic and industrial polypeptides in eukaryotic cells.
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