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    Systems using cell culture for production of isoprene
    1.
    发明授权
    Systems using cell culture for production of isoprene 有权
    使用细胞培养物生产异戊二烯的系统

    公开(公告)号：US09121039B2

    公开(公告)日：2015-09-01

    申请号：US14028342

    申请日：2013-09-16

    申请人： Danisco US Inc. ,  The Goodyear Tire & Rubber Company

    发明人： Gopal K. Chotani ,  Joseph C. McAuliffe ,  Caroline M. Peres ,  Gregory M. Whited

    IPC分类号： C12P5/02 ,  C12P5/00

    CPC分类号： C12P5/007 ,  C12P5/026

    摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

    摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。 本发明提供异戊二烯组合物，例如具有增加的异戊二烯量或增加的纯度的组合物。 另外，本发明提供了通过在适合异戊二烯生产的条件下培养细胞同时维持细胞活力和/或代谢活性来生产异戊二烯的方法。

    POLYMERS OF ISROPENE FROM RENEWABLE RESOURCES
    2.
    发明申请
    POLYMERS OF ISROPENE FROM RENEWABLE RESOURCES 有权
    可再生能源的废弃物聚合物

    公开(公告)号：US20130253141A1

    公开(公告)日：2013-09-26

    申请号：US13792832

    申请日：2013-03-11

    申请人： THE GOODYEAR TIRE & RUBBER COMPANY ,  DANISCO US INC.

    发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

    IPC分类号： C08F136/08 ,  C08F236/10 ,  C08F236/08

    CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

    摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.

    摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-220 / 00。