公开(公告)号：US20130253141A1

公开(公告)日：2013-09-26

申请号：US13792832

申请日：2013-03-11

申请人： THE GOODYEAR TIRE & RUBBER COMPANY ,  DANISCO US INC.

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F136/08 ,  C08F236/10 ,  C08F236/08

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.

摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-220 / 00。

公开(公告)号：US08940849B2

公开(公告)日：2015-01-27

申请号：US13792832

申请日：2013-03-11

申请人： The Goodyear Tire & Rubber Company ,  Danisco US Inc.

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F297/04 ,  C08F136/08 ,  C08F236/08 ,  C07C11/18 ,  C08F2/00 ,  C08F36/04 ,  C08L9/00 ,  C12P5/00 ,  C08F236/10 ,  C12P5/02 ,  C08F4/48

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.

摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22‰。