公开(公告)号：US07871773B2

公开(公告)日：2011-01-18

申请号：US11756765

申请日：2007-06-01

Applicant: Richard Haugland ,  Stephen Yue

Inventor： Richard Haugland ,  Stephen Yue

IPC: C12Q1/68 ,  C12Q1/00 ,  C12Q1/02 ,  G01N33/53 ,  C07D215/00 ,  C07D417/00 ,  C12P19/34

CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

公开(公告)号：US20080044811A1

公开(公告)日：2008-02-21

申请号：US11756765

申请日：2007-06-01

Applicant: Richard Haugland ,  Stephen Yue

Inventor： Richard Haugland ,  Stephen Yue

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/00 ,  G01N33/68

CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

公开(公告)号：US08252530B2

公开(公告)日：2012-08-28

申请号：US12984380

申请日：2011-01-04

Applicant: Richard Haugland ,  Stephen Yue

Inventor： Richard Haugland ,  Stephen Yue

IPC: C12Q1/68 ,  C12Q1/00 ,  C12Q1/02 ,  G01N33/53 ,  C07D215/00 ,  C07D417/00 ,  C12P19/34

CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

公开(公告)号：US08999965B2

公开(公告)日：2015-04-07

申请号：US13126234

申请日：2009-10-20

Applicant: Stephen Yue ,  Shih-Jung Huang ,  Jolene Bradford

Inventor： Stephen Yue ,  Shih-Jung Huang ,  Jolene Bradford

IPC: A61K31/33 ,  C12Q1/68 ,  C09B1/516 ,  G01N21/64

CPC classification number: G01N33/52 ,  C07C225/36 ,  C07C237/10 ,  C07C2603/24 ,  C07D205/04 ,  C07D213/36 ,  C07D233/61 ,  C07D249/04 ,  C07D295/13 ,  C07D295/192 ,  C07H21/04 ,  C09B1/5165 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N21/6428 ,  G01N2021/6441

Abstract: Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

Abstract translation: 近红外发射荧光化合物; 制备方法和含有所述化合物的试剂盒; 以及它们在荧光检测生物材料中的应用。

公开(公告)号：US08669374B2

公开(公告)日：2014-03-11

申请号：US13218395

申请日：2011-08-25

Applicant: Gene Shen ,  Stephen Yue

Inventor： Gene Shen ,  Stephen Yue

IPC: C07D209/02 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

Abstract: The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

Abstract translation: 本发明提供了一种新颖的花青染料，其可用连接体部分官能化，这些连接部分有助于它们与增加水溶性的其它物质和取代基结合，并优化染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。

公开(公告)号：US08569506B2

公开(公告)日：2013-10-29

申请号：US13556643

申请日：2012-07-24

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D215/00

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

公开(公告)号：US08470529B2

公开(公告)日：2013-06-25

申请号：US12851030

申请日：2010-08-05

Applicant: Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

Inventor： Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

IPC: A61K31/4709 ,  C07D215/12 ,  C12Q1/68

CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

公开(公告)号：US20130011927A1

公开(公告)日：2013-01-10

申请号：US13525690

申请日：2012-06-18

Applicant: Matthew Paul Beaudet ,  W. Gregory Cox ,  Stephen Yue

Inventor： Matthew Paul Beaudet ,  W. Gregory Cox ,  Stephen Yue

IPC: C09B23/04 ,  G01N21/64

CPC classification number: G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.

公开(公告)号：US08252932B2

公开(公告)日：2012-08-28

申请号：US12906304

申请日：2010-10-18

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07D215/00

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20110236887A1

公开(公告)日：2011-09-29

申请号：US13126150

申请日：2009-10-26

Applicant: Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

Inventor： Stephen Yue ,  Jixiang Liu ,  Jolene Bradford

IPC: C12Q1/68 ,  G01N33/50 ,  C07D265/38

CPC classification number: C07D265/38

Abstract: Actinomycin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided.

Abstract translation: 提供基于放线菌素的近红外发射化合物及其用作核酸污渍的方法。