公开(公告)号：US6039897A

公开(公告)日：2000-03-21

申请号：US919464

申请日：1997-08-28

Applicant: Michael J. Lochhead ,  Paul Yager

Inventor： Michael J. Lochhead ,  Paul Yager

IPC: B29C33/40 ,  B29C33/42 ,  B29C39/24 ,  B81C1/00 ,  G03F7/00 ,  B29D11/00

CPC classification number: B82Y10/00 ,  B29C33/405 ,  B29C33/42 ,  B29C39/24 ,  B81C1/00373 ,  B82Y40/00 ,  G03F7/0002 ,  B81C2201/0187

Abstract: A method for patterning a material onto a substrate including the steps of: providing a micro-mold having a plurality of non-communicating independent channels and having a plurality of reservoirs for receiving a micro-molding fluid each of which reservoirs communicates with a channel, the micro-mold including an elastomeric master having a surface with a plurality of recesses therein and a substrate; introducing a micro-molding fluid into the micro-mold reservoirs filling said communicating channels; and solidifying the fluid in the micro-mold and removing the elastomeric master thereby generating a pattern of material on the substrate. The micro-mold is formed by contacting the surface of the elastomeric master with the substrate such that the recesses in the surface form the plurality of reservoirs and channels.

Abstract translation: 一种用于将材料图案化到衬底上的方法，包括以下步骤：提供具有多个不连通的独立通道的微型模具，并具有多个用于容纳每个储存器与沟道连通的微型模塑流体的储存器， 所述微型模具包括弹性体主体，所述弹性体主体具有其中具有多个凹部的表面和基板; 将微型流体引入到填充所述连通通道的微型模具储存器中; 并固化微型模具中的流体并且移除弹性体主体，从而在衬底上产生材料图案。 微型模具通过使弹性体主体的表面与基底接触而形成，使得表面中的凹部形成多个储存器和通道。

公开(公告)号：US08697435B2

公开(公告)日：2014-04-15

申请号：US12871402

申请日：2010-08-30

Applicant: James R. Heil ,  Michael J. Lochhead ,  Kevin D. Moll ,  Christopher J. Myatt

Inventor： James R. Heil ,  Michael J. Lochhead ,  Kevin D. Moll ,  Christopher J. Myatt

IPC: C12M1/34 ,  G01N33/543

CPC classification number: G01N33/54326 ,  G01N33/54373 ,  G01N33/585 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806

Abstract: A system for sample preparation and analyte detection includes a cartridge, with a fluidic channel, a waveguide, and a capture spot. The system further includes a force field generator, an imaging system, and a fluid, which includes a sample potentially containing a target analyte, first type particles, which include binding moieties specific for the target analyte and are responsive to a force field, and second type particles, which include binding moieties specific for the target analyte and are capable of generating a signal. When the sample contains the target analyte, specific binding interactions between the target analyte and binding moieties link first and second type particles via the target analyte to form multiple-particle complex capturable at a capture spot. The force field allows manipulation of the particles and multiple-particle complex such that the detected signal from the second type particles is indicative of the target analyte within the sample.

Abstract translation: 用于样品制备和分析物检测的系统包括具有流体通道，波导和捕获点的盒。 该系统还包括力场发生器，成像系统和流体，其包括潜在地包含目标分析物的样品，第一类型颗粒，其包括对于目标分析物特异的并且对力场有反应的结合部分，并且第二 型颗粒，其包括对目标分析物特异性的结合部分并且能够产生信号。 当样品含有目标分析物时，目标分析物和结合部分之间的特异性结合相互作用通过目标分析物连接第一和第二类型颗粒，以在捕捉点处形成可以捕获的多重颗粒复合物。 力场允许操作颗粒和多颗粒复合物，使得来自第二类型颗粒的检测信号指示样品内的目标分析物。

公开(公告)号：US06844028B2

公开(公告)日：2005-01-18

申请号：US10180199

申请日：2002-06-25

Applicant: Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

Inventor： Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

IPC: B05D7/24 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N35/00 ,  G01N37/00 ,  B05D1/00

CPC classification number: G01N33/54393 ,  G01N33/5306 ,  G01N2035/00158 ,  Y10T428/31504 ,  Y10T428/31612 ,  Y10T428/31663 ,  Y10T428/31678

Abstract: Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

公开(公告)号：US20120288717A1

公开(公告)日：2012-11-15

申请号：US13468841

申请日：2012-05-10

Applicant: Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

Inventor： Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

IPC: B32B9/00

CPC classification number: G01N33/54393 ,  G01N33/5306 ,  G01N2035/00158 ,  Y10T428/31504 ,  Y10T428/31612 ,  Y10T428/31663 ,  Y10T428/31678

Abstract: Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

Abstract translation: 描述了制备具有低非特异性结合的功能性薄膜或表面涂层的组合物和方法。 薄膜含有特定的官能团和非特异性结合驱避剂组分。 薄膜共价结合或被动地吸附到各种固体基质上。 指定的官能团为薄膜改性的固体表面和非特异性结合驱除剂组分提供了特定的活性，显着降低了与薄膜改性固体表面的非特异性结合。 非特异性结合驱避剂组分不影响薄膜中指定的官能团活性。 在这些方法中，指定的官能团通过间隔基团固定在固体基质上。 还描述了表面涂层具有与特异性结合活性的官能团结合的非特异性蛋白质结合特性，从而提供特异性识别靶蛋白但限制与非特异性蛋白结合的表面涂层。

公开(公告)号：US20110012026A1

公开(公告)日：2011-01-20

申请号：US12883724

申请日：2010-09-16

Applicant: Kevin D. Moll ,  Kurt R. Vogel ,  Marie J. Delaney ,  Michael J. Lochhead ,  Christopher J. Myatt

Inventor： Kevin D. Moll ,  Kurt R. Vogel ,  Marie J. Delaney ,  Michael J. Lochhead ,  Christopher J. Myatt

IPC: G01N21/64 ,  G02B6/32

CPC classification number: G01N21/552 ,  G02B6/124 ,  G02B6/4206

Abstract: An apparatus for illuminating a sample includes a planar waveguide. The planar waveguide includes a first substrate, including a first outer surface and a first inner surface, and a second substrate, including a second outer surface and a second inner surface. The first and second inner surfaces of the first and second substrates, respectively, are spaced apart from each other and partly define a volume for confining the sample therein. The apparatus also includes a light source for providing light directed toward the planar waveguide, such that the light is optically coupled to and contained within the planar waveguide between the outer surfaces of the first and second substrates, while illuminating at least a portion of the sample confined within the volume.

公开(公告)号：US20100220318A1

公开(公告)日：2010-09-02

申请号：US12617535

申请日：2009-11-12

Applicant: Kevin D. Moll ,  Kurt R. Vogel ,  Marie J. Delaney ,  Michael J. Lochhead ,  Christopher I. Myatt

Inventor： Kevin D. Moll ,  Kurt R. Vogel ,  Marie J. Delaney ,  Michael J. Lochhead ,  Christopher I. Myatt

IPC: G01N21/41 ,  G02B6/32

CPC classification number: G01N21/552 ,  G02B6/12004 ,  G02B6/4206 ,  G02B2006/12102

Abstract: A sample can be illuminated for analysis using apparatus including a light source, a planar waveguide, and a refractive volume. The light source provides light along a propagation vector. The planar waveguide is oriented such that the propagation vector is perpendicular to the normal vector of the planar waveguide and offset from the planar waveguide in a direction parallel to the normal vector of the planar waveguide. The refractive volume is positioned proximate to the planar waveguide and can optically coupling light provided by the light source to the planar waveguide.

Abstract translation: 可以使用包括光源，平面波导和折射体积的装置照亮样品进行分析。 光源沿着传播矢量提供光。 平面波导取向为使得传播矢量垂直于平面波导的法线向量并且平行于平面波导的方向平行于平面波导的法向矢量。 折射体积位于平面波导附近，并且可将由光源提供的光光学耦合到平面波导。

公开(公告)号：US07067194B2

公开(公告)日：2006-06-27

申请号：US10718880

申请日：2003-11-21

Applicant: Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

Inventor： Guoqiano Mao ,  Steven W. Metzger ,  Michael J. Lochhead

IPC: B32B9/00

CPC classification number: G01N33/54393 ,  G01N33/5306 ,  G01N2035/00158 ,  Y10T428/31504 ,  Y10T428/31612 ,  Y10T428/31663 ,  Y10T428/31678

Abstract: Compositions and methods of preparing functional thin films or surface coatings with low non-specific binding are described. The thin films contain specified functional groups and non-specific binding repellant components. The thin films are either covalently bound to or passively adsorbed to various solid substrates. The specified functional group provides specified activity for the thin film modified solid surfaces and non-specific binding repellant components significantly reduce the non-specific binding to the thin film modified solid surfaces. Non-specific binding repellant components do not affect specified functional group's activity in the thin films. In these methods, specified functional groups are anchored to the solid substrates through a spacer. Surface coatings are also described having both non-specific protein binding properties combined with functional groups for specific binding activity thereby providing surface coating that specifically recognize target proteins but limit binding to non-specific protein.

公开(公告)号：US09341504B2

公开(公告)日：2016-05-17

申请号：US13878611

申请日：2011-10-11

Applicant: Michael J. Lochhead ,  Kurt R. Vogel ,  Keagan B. Rowley

Inventor： Michael J. Lochhead ,  Kurt R. Vogel ,  Keagan B. Rowley

IPC: G01N21/03 ,  G01N21/05 ,  G01F1/74 ,  G01F1/34 ,  B01L3/00

CPC classification number: G01F1/34 ,  B01L3/502723 ,  B01L3/50273 ,  B01L2200/0642 ,  B01L2300/069 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0688 ,  G01F1/74 ,  G01N2021/0346 ,  G01N2021/056

Abstract: A fluidic cartridge (110) with passively driven fluid flow is disclosed. The disclosed device and method solve problems associated with passive-flow fluidics by using a wicking pad (510) and/or a tilting means to prevent channel draining and backflow. The disclosure further provides means for adding liquid to the cartridge in multiple steps, without requiring liquid aspiration or removal.

Abstract translation: 公开了一种被动驱动的流体流动的流体盒（110）。 所公开的装置和方法通过使用芯吸垫（510）和/或倾斜装置来解决与被动流动流体相关的问题，以防止通道排水和回流。 本公开还提供了用于在不需要液体抽吸或除去的情况下以多个步骤将药液添加到药筒的装置。

公开(公告)号：US08586347B2

公开(公告)日：2013-11-19

申请号：US13233794

申请日：2011-09-15

Applicant: Michael J. Lochhead ,  Jeffrey Ives ,  Kathryn Todorof ,  Charles Greef ,  Marie J. Delaney ,  Kevin D. Moll ,  Kurt R. Vogel ,  Keagan B. Rowley ,  Evelyn S. Woodruff ,  John S. Dunn ,  Christopher J. Myatt ,  Daniel T. Nieuwlandt

Inventor： Michael J. Lochhead ,  Jeffrey Ives ,  Kathryn Todorof ,  Charles Greef ,  Marie J. Delaney ,  Kevin D. Moll ,  Kurt R. Vogel ,  Keagan B. Rowley ,  Evelyn S. Woodruff ,  John S. Dunn ,  Christopher J. Myatt ,  Daniel T. Nieuwlandt

IPC: C40B30/04 ,  G01N21/75 ,  C12Q1/68 ,  C12M1/00

CPC classification number: G01N33/54373 ,  B01L3/502715 ,  B01L9/52 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0825 ,  B01L2400/0406 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7703

Abstract: A rapid diagnostic system that delivers a panel of serologic assay results using a small amount of blood, serum, or plasma is described. The system includes a disposable cartridge and a reader instrument, based on planar waveguide imaging technology. The cartridge incorporates a microarray of recombinant antigens and antibody controls in a fluidic channel, providing multiple parallel fluorescence assay results for a single sample.

Abstract translation: 描述了使用少量血液，血清或血浆提供一组血清学测定结果的快速诊断系统。 该系统包括基于平面波导成像技术的一次性盒和读取器。 该盒在流体通道中结合重组抗原和抗体对照的微阵列，为单个样品提供多个平行的荧光测定结果。

公开(公告)号：US20120316077A1

公开(公告)日：2012-12-13

申请号：US13491380

申请日：2012-06-07

Applicant: Charles Greef ,  Michael J. Lochhead

Inventor： Charles Greef ,  Michael J. Lochhead

IPC: G01N21/64 ,  C40B30/04 ,  G01N33/53

CPC classification number: G01N33/54373

Abstract: Disclosed here is a system and method for analyzing and/or detecting one or more target analytes in a sample by using a competitive assay. A standard curve may be constructed using known amounts of a molecule that is identical or substantially identical to the target analyte. Signals obtained from the target analyte can be compared against the standard curve in order to determine the level of the target analyte in the sample. The disclosed methods may be used in a multiplexed analyte detection and quantitation system.

Abstract translation: 这里公开了通过使用竞争性测定来分析和/或检测样品中的一种或多种目标分析物的系统和方法。 可以使用已知量的与靶分析物相同或基本相同的分子构建标准曲线。 可以将从目标分析物获得的信号与标准曲线进行比较，以确定样品中目标分析物的水平。 所公开的方法可以用于复用的分析物检测和定量系统中。