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    ENZYMATIC PROCESS FOR ACRYLAMIDE REDUCTION IN FOODSTUFFS
    4.
    发明申请
    ENZYMATIC PROCESS FOR ACRYLAMIDE REDUCTION IN FOODSTUFFS 审中-公开
    食品中丙烯酰胺减少的酶学方法

    公开(公告)号：US20070166439A1

    公开(公告)日：2007-07-19

    申请号：US11539724

    申请日：2006-10-09

    申请人： Jorn Soe ,  Charlotte Poulsen ,  Thomas Rand

    发明人： Jorn Soe ,  Charlotte Poulsen ,  Thomas Rand

    IPC分类号： A23L1/31

    CPC分类号： A23L19/19 ,  A23L5/25 ,  A23L19/18

    摘要： There is provided a process for the prevention and/or reduction of acrylamide formation and/or acrylamide precursor formation in a foodstuff comprising (i) a protein, a peptide or an amino acid and (ii) a first reducing sugar; the process comprising the steps of: (a) contacting the foodstuff with a first enzyme capable of converting the first reducing sugar into a second reducing sugar; and (b) contacting the foodstuff with a second enzyme capable of oxidising a reducing group of the second reducing sugar.

    摘要翻译： 提供了一种用于预防和/或减少食品中丙烯酰胺形成和/或丙烯酰胺前体形成的方法，其包括（i）蛋白质，肽或氨基酸和（ii）第一还原糖; 该方法包括以下步骤：（a）使食物与能够将第一还原糖转化成第二还原糖的第一酶接触; 和（b）使食物与能够氧化第二还原糖还原基团的第二酶接触。

    VARIANT POLYPEPTIDES AND METHODS OF MAKING
    6.
    发明申请
    VARIANT POLYPEPTIDES AND METHODS OF MAKING 有权
    各种多样性和制备方法

    公开(公告)号：US20080070287A1

    公开(公告)日：2008-03-20

    申请号：US11852274

    申请日：2007-09-07

    申请人： Jorn Soe ,  Jorn Mikkelson ,  Arno Kreij

    发明人： Jorn Soe ,  Jorn Mikkelson ,  Arno Kreij

    IPC分类号： C12N5/04 ,  A21D13/04 ,  A23L1/32 ,  C12P7/64 ,  C12N9/10 ,  C12N15/00

    CPC分类号： C12P7/6463 ,  C12P7/6454

    摘要： The present invention relates to a method for the production of a variant lipid acyltransferase comprising the steps of: (i) selecting a parent enzyme which is a lipid acyltranserase enzyme; (ii) modifying one or more amino acids to produce a variant lipid acyltransferase; (iii) testing the activity of the variant lipid acyltransferase on a galactolipid and/or phospholipid and/or triglyceride substrate; (iv) selecting a variant enzyme with enhanced activity towards galactolipids compared with the parent enzyme; (v) providing a Bacillus licheniformis cell; (vi) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding said variant lipid acyltransferase; and (iii) expressing said variant lipid acyltransferase in the cell under the control of a promoter sequence. The variant lipid acyltransferase can undergo post-translations modification, truncation and/or clipping, i.e., to remove a signal peptide. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis.

    摘要翻译： 本发明涉及生产变体脂质酰基转移酶的方法，包括以下步骤：（i）选择脂质酰基转移酶的亲本酶; （ii）修饰一个或多个氨基酸以产生变体脂质酰基转移酶; （iii）测试变体脂质酰基转移酶对半乳糖脂和/或磷脂和/或甘油三酯底物的活性; （iv）与亲本酶相比，选择具有增强的对半乳糖脂的活性的变体酶; （v）提供地衣芽孢杆菌细胞; （vi）用编码所述变体脂质酰基转移酶的异源核苷酸序列转化地衣芽孢杆菌细胞; 和（iii）在启动子序列控制下的细胞中表达所述变体脂质酰基转移酶。 变体脂质酰基转移酶可经历翻译后修饰，截短和/或剪切，即去除信号肽。 此外，本发明还涉及地衣芽孢杆菌表达脂质酰基转移酶，包含异源脂质酰基转移酶的地衣芽孢杆菌宿主细胞的使用和包含编码脂质酰基转移酶的核苷酸序列的载体，所述核苷酸序列可操作地连接到与B同源的启动子序列 地衣芽孢杆菌

    Protein
    8.
    发明申请
    Protein 有权
    蛋白

    公开(公告)号：US20080038404A1

    公开(公告)日：2008-02-14

    申请号：US11519734

    申请日：2006-09-12

    申请人： Janne Brunstedt ,  Jorn Mikkelsen ,  Henrik Pedersen ,  Jorn Soe

    发明人： Janne Brunstedt ,  Jorn Mikkelsen ,  Henrik Pedersen ,  Jorn Soe

    IPC分类号： A21D8/04 ,  A23C9/12 ,  A23D7/00 ,  A23D9/00 ,  A23G3/34 ,  A23L1/105 ,  A23L1/212 ,  A23L1/22 ,  A23L1/24 ,  A23L1/322 ,  A23L1/39 ,  C07H21/04 ,  C12N9/02

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    摘要翻译： 与甘油三酯相比，极性脂质活性比例更高的真菌野生型脂肪分解酶，其中所述酶优选具有至少为4的磷脂：甘油三酯活性比。优选地，根据本发明的脂肪分解酶具有糖脂： 甘油三酯水解活性比至少为1.5。 在一个实施方案中，根据本发明的真菌脂肪分解酶包含如SEQ ID NO：1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列， 与其具有至少90％的同一性。 本发明还包括编码真菌脂肪分解酶的核酸，该核酸选自：（a）包含SEQ ID No.3，SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 （b）通过遗传密码的退化与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和（c）核酸，其包含与SEQ ID No.3，SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90％同一性的核苷酸序列。

    LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY
    9.
    发明申请
    LIPOLYTIC ENZYME: USES THEREOF IN THE FOOD INDUSTRY 失效
    脂肪酶：在食品工业中使用

    公开(公告)号：US20080131936A1

    公开(公告)日：2008-06-05

    申请号：US11623607

    申请日：2007-01-16

    申请人： Andrei Miasnikov ,  Jorn Soe ,  Jorn Mikkelsen ,  Mira Povelainen ,  Virve Pitkanen

    发明人： Andrei Miasnikov ,  Jorn Soe ,  Jorn Mikkelsen ,  Mira Povelainen ,  Virve Pitkanen

    IPC分类号： C12N9/20 ,  C07H21/00 ,  C12P19/00 ,  C11C1/02

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ ID NOs: 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID NO: 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence show in SEQ ID NO: 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID NO: 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID NO: 3.

    摘要翻译： 本发明包括在各种方法和用途中可以从以下属性之一的链霉菌，棒状杆菌和热嗜热菌获得的脂肪分解酶的用途，其中所述脂肪分解酶能够水解糖脂或磷脂或从糖脂转移酰基或 磷脂酰基受体。 优选地，用于这些方法和用途的脂肪分解酶包含如SEQ ID NO：4,5,7,8,12,14或16中任一项所示的氨基酸序列或具有至少70％ 同一性或包含如SEQ ID NO：3,6,9,13,15或17所示的核苷酸序列或与其具有至少70％同一性的核苷酸序列。 本发明还涉及能够至少水解半乳糖脂或能够将酰基从半乳糖脂转移至一种或多种酰基受体底物的脂肪分解酶，其中所述酶可从链霉菌属物种获得，并且包括含有氨基酸的脂肪分解酶 如SEQ ID NO：4所示的酸序列或与其具有至少60％同一性的氨基酸序列。 该酶可以由选自以下的核酸编码：a）包含SEQ ID NO：3所示核苷酸序列的核酸; b）通过遗传密码的退化与SEQ ID NO：3的核苷酸序列相关的核酸; 和c）包含与SEQ ID NO：3所示核苷酸序列具有至少70％同一性的核苷酸序列的核酸。

    ENZYMATIC OIL-DEGUMMING METHOD
    10.
    发明申请
    ENZYMATIC OIL-DEGUMMING METHOD 有权
    ENIYYY油脂脱水方法

    公开(公告)号：US20070298157A1

    公开(公告)日：2007-12-27

    申请号：US11623689

    申请日：2007-01-16

    申请人： Jorn Soe ,  Mark Turner

    发明人： Jorn Soe ,  Mark Turner

    IPC分类号： A23D9/00 ,  C12N9/10

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.

    摘要翻译： 一种酶促脱脂食用油的方法，包括用脂质酰基转移酶处理食用油，以将酰基从大部分磷脂转移到一个或多个酰基受体上，其中酰基受体可以是包含羟基的任何化合物。 在一个实施方案中，优选地，酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO： SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11，SEQ ID NO：12，SEQ ID NO：13， 14，或SEQ ID NO：15，SEQ ID NO：16，SEQ ID NO：17，SEQ ID NO：18，SEQ ID NO：36，SEQ ID NO：38，SEQ ID NO：40，SEQ ID NO：41 ，SEQ ID NO：45，SEQ ID NO：47，SEQ ID NO：50或与其同一性为75％以上的氨基酸序列。 还教导了包含SEQ ID NO：16所示氨基酸序列的新型脂质酰基转移酶。