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    Method of Producing Medium Chain Fatty Acid Using Beta-Ketoacyl-ACP Synthase
    5.
    发明申请
    Method of Producing Medium Chain Fatty Acid Using Beta-Ketoacyl-ACP Synthase 审中-公开

    公开(公告)号：US20170107545A1

    公开(公告)日：2017-04-20

    申请号：US15317347

    申请日：2015-07-30

    Applicant: Kao Corporation

    Inventor： Takuto TOJO ,  Shinji SUGIHARA

    IPC: C12P7/64 ,  C12N15/52 ,  C12N9/16 ,  C12N9/10

    CPC classification number: C12P7/6418 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

    Abstract: [Problems] To provide a method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component by using a β-ketoacyl-ACP synthase, and a gene, a protein and a transformant for use in this method.[Means to solve] A method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component, containing the steps of: introducing a gene encoding the following protein (A) or (B) into a host, and thereby obtaining a transformant, and collecting a medium chain fatty acid or a lipid containing this fatty acid as a component from the resulting transformant; and a gene, a protein and a transformant for use in this method: (A) A protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (B) A protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having medium chain acyl-ACP-specific β-ketoacyl-ACP synthase activity.

    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS
    6.
    发明申请
    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS 有权
    在脂肪酸合成中使用ACYL-ACYL载体蛋白质硫蛋白酶和突变体及其重组体的材料和方法

    公开(公告)号：US20160355793A1

    公开(公告)日：2016-12-08

    申请号：US15188965

    申请日：2016-06-21

    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

    Inventor： Basil J. Nikolau ,  Marna Yandeau-Nelson ,  Fuyuan Jing

    IPC: C12N9/16 ,  C12P7/64

    CPC classification number: C12N9/16 ,  C07K2319/00 ,  C12P7/6409 ,  C12P7/6418 ,  C12Y301/02014 ,  Y02P20/52

    Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

    Abstract translation: 一种增加脂肪酸生产的方法，其包括向宿主中引入并在其中表达来自甲酸杆状杆菌或其突变体的酰基酰基载体蛋白（ACP）硫酯酶（TE）; 制备突变型甲酸重组体酰基-ACP TE的方法; 制备嵌合的Cuphea viscosissima酰基-ACP TE的方法; 核酸分子，其包含编码突变型酰基-ACP TE的核苷酸序列或嵌合的Cuphea viscosissima酰基-ACP TE; 包含核酸分子的宿主; 突变型酰基-ACP TE或嵌合的Cuphea viscosissima酰基-ACP TE; 改变植物酰基-ACP TE对其底物中的至少一种的特异性的方法，包括向植物酰基ACP TE引入底物特异性改变突变; 以及改变植物酰基-ACP TE的活性水平的方法。

    Enzymatic process for fat and oil hydrolysis
    7.
    发明授权
    Enzymatic process for fat and oil hydrolysis 有权
    脂肪和油水解的酶法

    公开(公告)号：US09512451B2

    公开(公告)日：2016-12-06

    申请号：US14375380

    申请日：2013-01-30

    Applicant: Arvind Mallinath Lali ,  Annamma Anil Odaneth ,  Rajesh Natwarlal Vadgama ,  Anuradha Devdas Bhat ,  Amit Pande ,  Mrunal Anil Warke

    Inventor： Arvind Mallinath Lali ,  Annamma Anil Odaneth ,  Rajesh Natwarlal Vadgama ,  Anuradha Devdas Bhat ,  Amit Pande ,  Mrunal Anil Warke

    IPC: C12P7/64

    CPC classification number: C12P7/6418 ,  C12P7/64

    Abstract: An efficient process for enzymatic hydrolysis of fats and oils in a homogenous mixture is provided herein. The present invention in particular provides a process for production of fatty acids, sn-regio mono-acylglycerol (MAG), sn-regio di-acyl-glycerols (DAG), and glycerol from fats, wherein more than 98% fats can be converted into the desired product. The present invention also provides a process for the production of fatty acids and glycerol, virtually free of sn-regio diacyl-glycerols (DAG) and comprising less than 5% sn-regio mono-acylglycerol (MAG) in the end product.

    Abstract translation: 本文提供了在均匀混合物中脂肪和油的酶水解的有效方法。 本发明特别提供一种从脂肪酸生产脂肪酸，一种单一酰基甘油（MAG），二酰基 - 甘油二酯（DAG）和甘油的方法，其中可以转化超过98％的脂肪 进入所需的产品。 本发明还提供了一种生产脂肪酸和甘油的方法，其实际上没有二酰基 - 甘油（DAG），并且在最终产物中包含小于5％的sn-regio单酰基甘油（MAG）。

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