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    Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis
    3.
    发明授权
    Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis 有权
    在脂肪酸合成中使用酰基酰基载体蛋白硫酯酶及其突变体和嵌合体的材料和方法

    公开(公告)号：US09399768B2

    公开(公告)日：2016-07-26

    申请号：US14141327

    申请日：2013-12-26

    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

    Inventor： Basil J. Nikolau ,  Marna Yandeau-Nelson ,  Fuyuan Jing

    IPC: C12N9/16 ,  C12N1/20 ,  C12N1/12 ,  C07H21/04 ,  C12P7/64

    CPC classification number: C12N9/16 ,  C07K2319/00 ,  C12P7/6409 ,  C12P7/6418 ,  C12Y301/02014 ,  Y02P20/52

    Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

    Abstract translation: 一种增加脂肪酸生产的方法，其包括向宿主中引入并在其中表达来自甲酸杆状杆菌或其突变体的酰基酰基载体蛋白（ACP）硫酯酶（TE）; 制备突变型甲酸重组体酰基-ACP TE的方法; 制备嵌合的Cuphea viscosissima酰基-ACP TE的方法; 核酸分子，其包含编码突变型酰基-ACP TE的核苷酸序列或嵌合的Cuphea viscosissima酰基-ACP TE; 包含核酸分子的宿主; 突变型酰基-ACP TE或嵌合的Cuphea viscosissima酰基-ACP TE; 改变植物酰基-ACP TE对其底物中的至少一种的特异性的方法，包括向植物酰基ACP TE引入底物特异性改变突变; 以及改变植物酰基-ACP TE的活性水平的方法。

    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS
    5.
    发明申请
    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS 审中-公开
    在脂肪酸合成中使用ACYL-ACYL载体蛋白质硫蛋白酶和突变体及其重组体的材料和方法

    公开(公告)号：US20150322466A1

    公开(公告)日：2015-11-12

    申请号：US14599528

    申请日：2015-01-18

    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

    Inventor： Basil J. Nikolau ,  Marna Yandeau-Nelson ,  Fuyuan Jing

    IPC: C12P7/64 ,  C12N9/16

    CPC classification number: C12N9/16 ,  C07K2319/00 ,  C12P7/6409 ,  C12Y301/02014

    Abstract: A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimeric C. viscosissima acyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimeric C. viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.

    Abstract translation: 一种增加脂肪酸生产的方法，其包括向宿主细胞或生物体中引入并在其中表达来自甲酸杆菌或其突变体的酰基酰基载体蛋白（ACP）硫酯酶（TE）; 制备突变型甲酸重组体酰基-ACP TE的方法; 制备嵌合的Cuphea viscosissima酰基-ACP TE的方法; 编码突变型酰基-ACP TE或嵌合C粘稠体酰基-ACP TE的核酸; 包含该核酸的宿主细胞或生物体; 突变的酰基-ACP TE或嵌合的C.粘性酰基-ACP TE; 改变植物酰基-ACP TE的特异性的方法; 以及改变植物酰基-ACP TE的活性水平的方法。

    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS
    9.
    发明申请
    MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS 有权
    在脂肪酸合成中使用ACYL-ACYL载体蛋白质硫蛋白酶和突变体及其重组体的材料和方法

    公开(公告)号：US20160355793A1

    公开(公告)日：2016-12-08

    申请号：US15188965

    申请日：2016-06-21

    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

    Inventor： Basil J. Nikolau ,  Marna Yandeau-Nelson ,  Fuyuan Jing

    IPC: C12N9/16 ,  C12P7/64

    CPC classification number: C12N9/16 ,  C07K2319/00 ,  C12P7/6409 ,  C12P7/6418 ,  C12Y301/02014 ,  Y02P20/52

    Abstract: A method of increasing production of fatty acids comprising introducing into a host and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid molecule comprising a nucleotide sequence encoding a mutant acyl-ACP TE or a chimeric Cuphea viscosissima acyl-ACP TE; a host comprising the nucleic acid molecule; a mutant acyl-ACP TE or chimeric Cuphea viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE for at least one of its substrates comprising introducing into the plant acyl-ACP TE a substrate specificity-altering mutation; and a method of altering the level of activity of a plant acyl-ACP TE.

    Abstract translation: 一种增加脂肪酸生产的方法，其包括向宿主中引入并在其中表达来自甲酸杆状杆菌或其突变体的酰基酰基载体蛋白（ACP）硫酯酶（TE）; 制备突变型甲酸重组体酰基-ACP TE的方法; 制备嵌合的Cuphea viscosissima酰基-ACP TE的方法; 核酸分子，其包含编码突变型酰基-ACP TE的核苷酸序列或嵌合的Cuphea viscosissima酰基-ACP TE; 包含核酸分子的宿主; 突变型酰基-ACP TE或嵌合的Cuphea viscosissima酰基-ACP TE; 改变植物酰基-ACP TE对其底物中的至少一种的特异性的方法，包括向植物酰基ACP TE引入底物特异性改变突变; 以及改变植物酰基-ACP TE的活性水平的方法。

    MATERIALS AND METHODS FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS IN RECOMBINANT BACTERIA
    10.
    发明申请
    MATERIALS AND METHODS FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS IN RECOMBINANT BACTERIA 审中-公开
    在重组细菌中生产双功能脂肪酸的材料和方法

    公开(公告)号：US20160168603A1

    公开(公告)日：2016-06-16

    申请号：US14954972

    申请日：2015-11-30

    Applicant: OMEGACHEA BIORENEWABLES LLC ,  IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

    Inventor： Shivani Garg ,  Xiaochen Yu ,  Huanan Jin ,  Marna Yandeau-Nelson ,  Basil Nikolau ,  Ludmila Rizhsky

    IPC: C12P7/64 ,  C12N9/10 ,  C12N15/70

    CPC classification number: C12P7/6409 ,  C12N9/1029 ,  C12N15/52 ,  C12Y203/0118

    Abstract: A method of producing bi-functional fatty acids comprising introducing into a host cell or organism, which comprises one or more ω- or ω-1 functionalized acyl-CoAs, and expressing therein a KASIII, which can use one or more of the ω- or ω-1 functionalized acyl-CoAs as a substrate; a method of producing a ω-1 hydroxy branched fatty acid, a ω-1 branched fatty acid, or a combination thereof by culturing a mutant E. coli, which does not express a functional KASIII from the endogenous fabH gene and expresses a phaA and a phaB and a functional exogenous KASIII; and a mutant E. coli, a method of making the mutant, a culture comprising the mutant, and a composition comprising ω-1 hydroxy branched fatty acids, a ω-1 branched fatty acids, or a combination thereof obtained from the culture.

    Abstract translation: 一种生产双功能脂肪酸的方法，包括将包含一个或多个ω-或ω-1官能化酰基-CoAs的宿主细胞或生物体引入并表达KASIII，KASIII可以使用一个或多个ω- 或ω-1官能化酰基-CoAs作为底物; 通过培养不从内源fabH基因表达功能性KASIII并表达phaA的突变大肠杆菌来生产ω-1羟基支链脂肪酸，ω-1支链脂肪酸或其组合的方法， phaB和功能性外源KASIII; 和突变型大肠杆菌，制备突变体的方法，包含突变体的培养物，以及由培养物获得的包含ω-1羟基支链脂肪酸，ω-1支链脂肪酸或其组合的组合物。

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