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公开(公告)号：US20180215835A1
公开(公告)日：2018-08-02
申请号：US15876604
申请日：2018-01-22
Applicant: Kaneka Corporation
Inventor： Masakatsu Nishihachijyo , Yoshiyuki Nakano , Fuminori Konoike , Masayuki Takano , Shinichi Yoshida , Kazunobu Minakuchi
CPC classification number: C07K17/02 , B01D15/3809 , C07B2200/11 , C07K1/22 , C07K14/31 , C07K16/065 , C07K2317/52 , C07K2317/92 , C07K2318/20
Abstract: A protein includes an amino acid sequence derived from the sequence of SEQ ID NO: 1, wherein the amino acid sequence includes a substitution of Val at a position corresponding to position 40 of SEQ ID NO: 1 with a polar uncharged amino acid residue, a basic amino acid residue, or Ala. The protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.
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公开(公告)号：US10457705B2
公开(公告)日：2019-10-29
申请号：US16227688
申请日：2018-12-20
Applicant: KANEKA CORPORATION
Inventor： Takahiro Okubo , Yoshikazu Kawai , Masaru Hirano , Fuminori Konoike , Keiichi Karasugi , Tatsuya Honda
IPC: B01J20/24 , C07K1/22 , B01J20/30 , C08B1/08 , C08B15/08 , C08B15/10 , C08B3/06 , C07K16/00 , B01J20/26 , B01J20/285 , C08J3/16 , B01J20/28 , B01J20/32 , B01J20/286 , B01D15/38 , C08L1/12 , C08L1/04 , C08L1/02 , C08B16/00 , C08B15/00 , C08J9/00
Abstract: A carrier for ligand immobilization obtained by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate (%)=(1−V2/V1)×100 (wherein, V1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V2 indicates the gel volume of polysaccharide porous beads after shrinkage).
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公开(公告)号：US20180215796A1
公开(公告)日：2018-08-02
申请号：US15876610
申请日：2018-01-22
Applicant: Kaneka Corporation
Inventor： Masakatsu Nishihachijyo , Yoshiyuki Nakano , Fuminori Konoike , Masayuki Takano , Shinichi Yoshida , Kazunobu Minakuchi
CPC classification number: C07K14/31 , B01D15/3809 , B01J20/286 , B01J20/3274 , C07K1/22 , C07K17/00 , C07K2319/30
Abstract: A protein includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence includes a substitution of a hydrophobic amino acid residue, an acidic amino acid residue, or a polar uncharged amino acid residue with a basic amino acid residue, and the protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.
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4.发明申请公开(公告)号：US20180215795A1
公开(公告)日：2018-08-02
申请号：US15876597
申请日：2018-01-22
Applicant: Kaneka Corporation
Inventor： Masakatsu Nishihachijyo , Yoshiyuki Nakano , Fuminori Konoike , Masayuki Takano , Shinichi Yoshida , Kazunobu Minakuchi
CPC classification number: C07K14/31 , C07K1/22 , C07K14/195 , C07K17/00 , C07K19/00 , C07K2319/30 , C12N5/10 , C12N15/09
Abstract: A protein includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence includes a substitution of a hydrophobic amino acid residue in an Fc binding site with a different hydrophobic amino acid residue or a polar uncharged amino acid residue, and the protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.
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公开(公告)号：US09920098B2
公开(公告)日：2018-03-20
申请号：US14429819
申请日：2013-09-24
Applicant: Kaneka Corporation
Inventor： Shinichi Yoshida , Dai Murata , Fuminori Konoike , Keita Iguchi , Tomoyuki Nakaishi , Masahiro Hayashi
IPC: B01D15/38 , B01J20/26 , C07K1/22 , C07K14/31 , C07K16/06 , C07K17/00 , C07K16/00 , C07K16/12 , C07K17/12
CPC classification number: C07K14/31 , B01D15/3809 , B01J2220/4856 , C07K1/22 , C07K16/00 , C07K16/065 , C07K16/1271 , C07K17/00 , C07K17/12 , C07K2317/21 , C07K2317/52 , C07K2317/92
Abstract: An object of the present invention is to develop techniques to create novel engineered protein ligands that maximize the binding capacity and binding efficiency to a target molecule of affinity separation matrices on which the protein ligands are immobilized. The present invention provides protein ligands (variants) that can be immobilized on carriers in a manner shown in schematic FIG. 1(4)-(15), as well as antibody affinity separation matrices obtained by immobilizing such a protein ligand on a water-insoluble carrier. The affinity separation matrices are characterized by their excellent binding capacity and binding efficiency to a target molecule.
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Patent Agency Ranking
公开(公告)号：US20160168209A1
公开(公告)日：2016-06-16
申请号：US14429819
申请日：2013-09-24
Applicant: KANEKA CORPORATION
Inventor： Shinichi Yoshida , Dai Murata , Fuminori Konoike , Keita Iguchi , Tomoyuki Nakaishi , Masahiro Hayashi
CPC classification number: C07K14/31 , B01D15/3809 , B01J2220/4856 , C07K1/22 , C07K16/00 , C07K16/065 , C07K16/1271 , C07K17/00 , C07K17/12 , C07K2317/21 , C07K2317/52 , C07K2317/92
Abstract: An object of the present invention is to develop techniques to create novel engineered protein ligands that maximize the binding capacity and binding efficiency to a target molecule of affinity separation matrices on which the protein ligands are immobilized. The present invention provides protein ligands (variants) that can be immobilized on carriers in a manner shown in schematic FIG. 1(4)-(15), as well as antibody affinity separation matrices obtained by immobilizing such a protein ligand on a water-insoluble carrier. The affinity separation matrices are characterized by their excellent binding capacity and binding efficiency to a target molecule.
Abstract translation: 本发明的一个目的是开发技术来产生新的工程化蛋白质配体，其最大限度地增加了与蛋白质配体固定在其上的亲和分离基质的靶分子的结合能力和结合效率。 本发明提供蛋白质配体（变体），其可以以示意图所示的方式固定在载体上。 1（4） - （15），以及通过将这种蛋白质配体固定在水不溶性载体上获得的抗体亲和力分离基质。 亲和力分离基质的特征在于其与靶分子的优异的结合能力和结合效率。
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公开(公告)号：US10221211B2
公开(公告)日：2019-03-05
申请号：US15025152
申请日：2014-09-26
Applicant: KANEKA CORPORATION
Inventor： Takahiro Okubo , Yoshikazu Kawai , Masaru Hirano , Fuminori Konoike , Keiichi Karasugi , Tatsuya Honda
IPC: B01D15/38 , C07K1/22 , B01J20/24 , C08B1/08 , C08B15/08 , C08B15/10 , C08B3/06 , C08B15/00 , C08B16/00 , C08L1/02 , C08L1/04 , C08L1/12 , B01J20/286 , B01J20/32 , B01J20/28 , C08J3/16 , B01J20/26 , B01J20/285 , B01J20/30 , C07K16/00 , C08J9/00
Abstract: A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of: a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature, b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and d) bringing the cellulose slurry into contact with coagulation solvent. A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate (%)=(1−V2/V1)×100 (wherein, V1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V2 indicates the gel volume of polysaccharide porous beads after shrinkage).
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公开(公告)号：US20180215836A1
公开(公告)日：2018-08-02
申请号：US15883569
申请日：2018-01-30
Applicant: Kaneka Corporation
Inventor： Masakatsu Nishihachijyo , Fuminori Konoike , Yoshiyuki Nakano , Masayuki Takano , Keita Yamashita
CPC classification number: C07K17/02 , B01D15/3809 , B01J20/286 , B01J20/3204 , B01J20/321 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/065 , C07K16/42 , C07K2317/52 , C07K2317/92 , C12N15/09
Abstract: A protein includes two or more amino acid sequences, wherein each amino acid sequence is derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence closest to the N-terminus includes more Lys residues than the other amino acid sequence(s), and in the amino acid sequence closest to the N-terminus, a total number of Lys in positions 1 to 38 is equal to or greater than a total number of Lys in position 39 and subsequent positions.
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公开(公告)号：US20180215785A1
公开(公告)日：2018-08-02
申请号：US15876615
申请日：2018-01-22
Applicant: Kaneka Corporation
Inventor： Masakatsu Nishihachijyo , Yoshiyuki Nakano , Fuminori Konoike , Masayuki Takano , Shinichi Yoshida , Kazunobu Minakuchi
CPC classification number: C07K1/22 , B01D15/168 , B01D15/3809 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K14/31 , C07K17/02
Abstract: A method for purifying an antibody-like protein includes adsorbing an antibody-like protein onto an affinity separation matrix by bringing the antibody-like protein into contact with the affinity separation matrix; and eluting the antibody-like protein by bringing an eluent having a pH of 3.5 or higher into contact with the affinity separation matrix. The affinity separation matrix includes a carrier and a ligand immobilized on the carrier, and the ligand includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID Nos: 1 to 5. Gln or Lys in an Fc-binding site of the amino acid sequence is substituted by Ala, Ser, or Thr, and the ligand has a lower antibody-binding capacity in an acidic pH range, as compared to a ligand including the amino acid sequence without the substitution.
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公开(公告)号：US20180179250A1
公开(公告)日：2018-06-28
申请号：US15905635
申请日：2018-02-26
Applicant: Kaneka Corporation
Inventor： Fuminori Konoike , Tatsuya Honda , Keiichi Karasugi
IPC: C07K1/22 , C07K17/12 , C07K17/10 , C07K17/08 , C07K17/14 , B01J20/24 , B01J20/26 , B01J20/32 , B01D15/38
CPC classification number: C07K1/22 , B01D15/38 , B01D15/3809 , B01J20/24 , B01J20/265 , B01J20/286 , B01J20/3204 , B01J20/3208 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3229 , B01J20/3274 , B01J20/3278 , C07K16/00 , C07K16/06 , C07K17/08 , C07K17/10 , C07K17/12 , C07K17/14
Abstract: A method for immobilizing a ligand on a formyl group-containing insoluble base material includes producing an imine by mixing the ligand and the formyl group-containing insoluble base material, and reducing the imine by using a borane complex, wherein the ligand comprises an amino group and has a specific affinity for a target compound, and wherein the borane complex has a Lewis base ligand having pKa of 6.5 or less.
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