公开(公告)号：US20180215796A1

公开(公告)日：2018-08-02

申请号：US15876610

申请日：2018-01-22

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Yoshiyuki Nakano ,  Fuminori Konoike ,  Masayuki Takano ,  Shinichi Yoshida ,  Kazunobu Minakuchi

IPC: C07K14/31 ,  C07K1/22

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3274 ,  C07K1/22 ,  C07K17/00 ,  C07K2319/30

Abstract: A protein includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence includes a substitution of a hydrophobic amino acid residue, an acidic amino acid residue, or a polar uncharged amino acid residue with a basic amino acid residue, and the protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.

公开(公告)号：US20180215795A1

公开(公告)日：2018-08-02

申请号：US15876597

申请日：2018-01-22

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Yoshiyuki Nakano ,  Fuminori Konoike ,  Masayuki Takano ,  Shinichi Yoshida ,  Kazunobu Minakuchi

IPC: C07K14/31 ,  C07K1/22

CPC classification number: C07K14/31 ,  C07K1/22 ,  C07K14/195 ,  C07K17/00 ,  C07K19/00 ,  C07K2319/30 ,  C12N5/10 ,  C12N15/09

Abstract: A protein includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence includes a substitution of a hydrophobic amino acid residue in an Fc binding site with a different hydrophobic amino acid residue or a polar uncharged amino acid residue, and the protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.

公开(公告)号：US20180215835A1

公开(公告)日：2018-08-02

申请号：US15876604

申请日：2018-01-22

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Yoshiyuki Nakano ,  Fuminori Konoike ,  Masayuki Takano ,  Shinichi Yoshida ,  Kazunobu Minakuchi

IPC: C07K17/02 ,  C07K1/22 ,  B01D15/38 ,  C07K16/06

CPC classification number: C07K17/02 ,  B01D15/3809 ,  C07B2200/11 ,  C07K1/22 ,  C07K14/31 ,  C07K16/065 ,  C07K2317/52 ,  C07K2317/92 ,  C07K2318/20

Abstract: A protein includes an amino acid sequence derived from the sequence of SEQ ID NO: 1, wherein the amino acid sequence includes a substitution of Val at a position corresponding to position 40 of SEQ ID NO: 1 with a polar uncharged amino acid residue, a basic amino acid residue, or Ala. The protein has a reduced antibody-binding capacity in an acidic pH range, as compared to a protein including the amino acid sequence without the substitution.

公开(公告)号：US20210024433A1

公开(公告)日：2021-01-28

申请号：US17039471

申请日：2020-09-30

Applicant: KANEKA CORPORATION

Inventor： Takayuki Asada ,  Yoshiyuki Nakano ,  Toyoaki Watanabe ,  Ken Uekita

IPC: C05G5/10

Abstract: One or more embodiments of the present invention provide an oxidized glutathione-containing solid composition which can be produced by a simple method and has low deliquescence and is easy to handle. One or more embodiments of the present invention relate to a solid composition comprising an amorphous oxidized glutathione and a water-soluble cellulose derivative. One or more embodiments of the present invention also relate to a method for producing the solid composition comprising an amorphous oxidized glutathione and a water-soluble cellulose derivative, comprising a step of drying a solution comprising oxidized glutathione and a water-soluble cellulose derivative dissolved in an aqueous solvent.

公开(公告)号：US20190263870A1

公开(公告)日：2019-08-29

申请号：US16298639

申请日：2019-03-11

Applicant: KANEKA CORPORATION

Inventor： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

IPC: C07K14/195 ,  C07K16/32 ,  C07K14/31 ,  C07K16/06 ,  C07K1/22

Abstract: An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

公开(公告)号：US20180215836A1

公开(公告)日：2018-08-02

申请号：US15883569

申请日：2018-01-30

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Fuminori Konoike ,  Yoshiyuki Nakano ,  Masayuki Takano ,  Keita Yamashita

IPC: C07K17/02 ,  C07K1/22 ,  B01D15/38 ,  C07K16/06 ,  B01J20/32

CPC classification number: C07K17/02 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/31 ,  C07K16/065 ,  C07K16/42 ,  C07K2317/52 ,  C07K2317/92 ,  C12N15/09

Abstract: A protein includes two or more amino acid sequences, wherein each amino acid sequence is derived from a sequence selected from the group consisting of SEQ ID NOs: 1 to 5. The amino acid sequence closest to the N-terminus includes more Lys residues than the other amino acid sequence(s), and in the amino acid sequence closest to the N-terminus, a total number of Lys in positions 1 to 38 is equal to or greater than a total number of Lys in position 39 and subsequent positions.

公开(公告)号：US20180215785A1

公开(公告)日：2018-08-02

申请号：US15876615

申请日：2018-01-22

Applicant: Kaneka Corporation

Inventor： Masakatsu Nishihachijyo ,  Yoshiyuki Nakano ,  Fuminori Konoike ,  Masayuki Takano ,  Shinichi Yoshida ,  Kazunobu Minakuchi

IPC: C07K1/22 ,  C07K14/31

CPC classification number: C07K1/22 ,  B01D15/168 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K14/31 ,  C07K17/02

Abstract: A method for purifying an antibody-like protein includes adsorbing an antibody-like protein onto an affinity separation matrix by bringing the antibody-like protein into contact with the affinity separation matrix; and eluting the antibody-like protein by bringing an eluent having a pH of 3.5 or higher into contact with the affinity separation matrix. The affinity separation matrix includes a carrier and a ligand immobilized on the carrier, and the ligand includes an amino acid sequence derived from a sequence selected from the group consisting of SEQ ID Nos: 1 to 5. Gln or Lys in an Fc-binding site of the amino acid sequence is substituted by Ala, Ser, or Thr, and the ligand has a lower antibody-binding capacity in an acidic pH range, as compared to a ligand including the amino acid sequence without the substitution.