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    Modified Fab region-binding peptide
    2.
    发明授权
    Modified Fab region-binding peptide 有权

    公开(公告)号：US10774115B2

    公开(公告)日：2020-09-15

    申请号：US15686666

    申请日：2017-08-25

    Applicant: Kaneka Corporation

    Inventor： Shinichi Yoshida

    IPC: C07K14/31 ,  C07K14/315 ,  C12N15/09 ,  G01N30/88 ,  C07K16/24 ,  A61K39/44 ,  A61K38/00 ,  A61K39/00 ,  C07K19/00 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38

    Abstract: A first Fab region-binding peptide includes an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 5 with substitution of one or more amino acid residues at the 17th position and the 36th position, wherein an acid dissociation pH thereof is shifted to a neutral side. A second Fab region-binding peptide further includes deletion, substitution and/or addition of one or more amino acid residues at positions other than the 17th position and the 36th position. A third Fab region-binding peptide includes an amino acid sequence with a sequence identity of 80% or more to the amino acid sequence of the first Fab region-binding peptide.

    MUTANT IMMUNOGLOBULIN K CHAIN VARIABLE REGION-BINDING PEPTIDE
    4.
    发明申请
    MUTANT IMMUNOGLOBULIN K CHAIN VARIABLE REGION-BINDING PEPTIDE 审中-公开

    公开(公告)号：US20180016306A1

    公开(公告)日：2018-01-18

    申请号：US15660365

    申请日：2017-07-26

    Applicant: Kaneka Corporation

    Inventor： Shinichi Yoshida

    IPC: C07K14/195 ,  C07K16/00 ,  C07K1/22 ,  C07K16/10 ,  C07K17/00

    Abstract: A first immunoglobulin κ chain variable region-binding peptide includes an amino acid sequence of SEQ ID NO: 21 with substitution of one or more amino acid residues at the 15th position, the 16th position, the 17th position or the 18th position, wherein an acid dissociation pH thereof is shifted to a neutral side. A second immunoglobulin κ chain variable region-binding peptide further includes deletion, substitution and/or addition of 1-20 amino acid residues at positions other than the 15th position, the 16th position, the 17th position and the 18th position. A third immunoglobulin κ chain variable region-binding peptide includes an amino acid sequence with a sequence identity of 80% or more to the amino acid sequence of the first immunoglobulin κ chain variable region-binding peptide.

    Fab region-binding peptide
    6.
    发明授权
    Fab region-binding peptide 有权

    公开(公告)号：US10556944B2

    公开(公告)日：2020-02-11

    申请号：US14914439

    申请日：2014-08-28

    Applicant: KANEKA CORPORATION

    Inventor： Shinichi Yoshida ,  Dai Murata

    IPC: C07K16/12 ,  C07K14/315 ,  C07K1/22

    Abstract: An object of the present invention is to provide a Fab region-binding peptide having an excellent ability for binding to a Fab region of IgG, an affinity separation matrix having the peptide as a ligand, and a method for producing a Fab region-containing protein, the method using the affinity separation matrix. Further, another object of the present invention is to provide a DNA encoding for the peptide, a vector containing the DNA, and a transformant which has been transformed by the vector. The Fab region-binding peptide according to the present invention is characterized in having a mutation at a specific site in comparison with wild-type SpG-β1.

    METHOD FOR PRODUCING ANTIBODY FRAGMENT
    8.
    发明申请
    METHOD FOR PRODUCING ANTIBODY FRAGMENT 审中-公开

    公开(公告)号：US20190153072A1

    公开(公告)日：2019-05-23

    申请号：US16259174

    申请日：2019-01-28

    Applicant: KANEKA CORPORATION

    Inventor： Dai Murata ,  Shinichi Yoshida

    IPC: C07K16/06 ,  B01D15/38

    Abstract: A method for producing an antibody fragment includes preparing a liquid sample that includes the antibody fragment and does not include an Fc fragment, adsorbing the antibody fragment on an affinity separation matrix by contacting the liquid sample with the affinity separation matrix, removing impurities from the affinity separation matrix by washing the affinity separation matrix, and separating the antibody fragment from the affinity separation matrix. The antibody fragment includes a CH1 region and does not include an Fc region. The affinity separation matrix includes a water-insoluble carrier and a ligand immobilized on the water-insoluble carrier. The ligand is Protein G, a Protein G domain, a Protein G variant or a Protein G domain variant.

    METHOD FOR PRODUCING PROTEIN INCLUDING k CHAIN VARIABLE REGION
    9.
    发明申请
    METHOD FOR PRODUCING PROTEIN INCLUDING k CHAIN VARIABLE REGION 审中-公开

    公开(公告)号：US20190119362A1

    公开(公告)日：2019-04-25

    申请号：US16176090

    申请日：2018-10-31

    Applicant: KANEKA CORPORATION

    Inventor： Shinichi Yoshida ,  Dai Murata

    IPC: C07K16/06 ,  C07K1/22

    Abstract: A method for producing a protein includes adsorbing a protein including a κ chain variable region on an insoluble carrier of an affinity separation matrix by contacting a liquid sample including the protein with the affinity separation matrix; washing the affinity separation matrix to remove impurities; separating the protein from the affinity separation matrix by using an acidic buffer; and regenerating the affinity separation matrix by using an alkaline aqueous solution after the protein is separated from the affinity separation matrix. The insoluble carrier includes a ligand immobilized on the insoluble carrier, and the ligand is a κ chain variable region-binding peptide including B5 domain of Protein L derived from Peptostreptococcus magnus 312 strain or a variant of the B5 domain. The adsorbing, the washing, and the separating are repeated 3 or more times.

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