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168 条记录 (耗时 0.027 秒)

    Affinity resin
    2.
    发明申请
    Affinity resin 有权
    亲和树脂

    公开(公告)号：US20070167594A1

    公开(公告)日：2007-07-19

    申请号：US10573165

    申请日：2004-10-15

    申请人： Akito Tanaka ,  Takaaki Shiyama ,  Akira Yamazaki

    发明人： Akito Tanaka ,  Takaaki Shiyama ,  Akira Yamazaki

    IPC分类号： C08F26/00 ,  C08F126/00 ,  C08F226/00 ,  C07C69/73

    CPC分类号： G01N33/53 ,  A61K6/083 ,  B01D15/3804 ,  B01J20/267 ,  B01J20/289 ,  C08F220/36 ,  C08L33/10 ,  G01N33/543 ,  G01N33/545

    摘要： A resin which is prepared by polymerizing a monomer component incorporating a hydrophilic spacer, and a ligand-immobilized solid phase carrier obtained by immobilizing a ligand to the resin, are capable of reducing the non-specific adsorption of substances, other than the target molecule for the ligand, which mingle in the sample, to the resin and/or the ligand. Therefore, target molecule search, identification and the like with less noise are enabled.

    摘要翻译： 通过聚合包含亲水间隔基的单体组分和通过将配体固定在树脂上获得的配体固定的固相载体制备的树脂能够降低除了靶分子以外的物质的非特异性吸附 在样品中混合的配体与树脂和/或配体结合。 因此，能够实现具有较少噪声的靶分子搜索，识别等。

    Method of inhibiting nonspecific interaction between molecules on solid phase support
    5.
    发明申请
    Method of inhibiting nonspecific interaction between molecules on solid phase support 有权
    抑制固相载体上分子之间非特异性相互作用的方法

    公开(公告)号：US20060177943A1

    公开(公告)日：2006-08-10

    申请号：US10522716

    申请日：2003-07-30

    申请人： Akito Tanaka ,  Tomohiro Terada ,  Tsuruki Tamura ,  Takaaki Shiyama ,  Akira Yamazaki ,  Minoru Furuya ,  Masayuki Haramura

    发明人： Akito Tanaka ,  Tomohiro Terada ,  Tsuruki Tamura ,  Takaaki Shiyama ,  Akira Yamazaki ,  Minoru Furuya ,  Masayuki Haramura

    IPC分类号： G01N33/543 ,  C07C229/64

    CPC分类号： C08G65/33396 ,  G01N33/54393

    摘要： The present invention provides a method of suppressing the nonspecific interaction between molecules, characterized in that in a process to immobilize a molecule onto a solid phase carrier and analyze the specific interaction between the molecule and a molecule that specifically interacts with the molecule on the solid phase, the hydrophobic property of the solid phase surface in the solid phase carrier is regulated, particularly a hydrophilic spacer is interlaid at the time of immobilization of the molecule onto the solid phase carrier, which method makes it possible to suppress the nonspecific interaction between the molecules, and to reduce nonspecific adsorption to the solid phase.

    摘要翻译： 本发明提供了抑制分子之间非特异性相互作用的方法，其特征在于在将分子固定在固相载体上并分析分子与与固相分子特异性相互作用的分子之间的特异性相互作用的方法 调节固相载体中固相表面的疏水性，特别是在将分子固定在固相载体上时，亲水间隔物被嵌入，该方法可以抑制分子之间的非特异性相互作用 ，并减少对固相的非特异性吸附。

    Method of calibrating ligand specificity
    7.
    发明申请
    Method of calibrating ligand specificity 失效
    校准配体特异性的方法

    公开(公告)号：US20070122913A1

    公开(公告)日：2007-05-31

    申请号：US10573167

    申请日：2004-10-15

    申请人： Akito Tanaka ,  Akira Yamazaki

    发明人： Akito Tanaka ,  Akira Yamazaki

    IPC分类号： G01N33/558

    CPC分类号： G01N33/54313 ,  G01N33/54393 ,  Y10S435/973 ,  Y10T436/10 ,  Y10T436/25375

    摘要： In a method comprising comparing a solid phase carrier extract 1 obtained by pre-treating a sample with a ligand-immobilized solid phase carrier and a solid phase carrier extract 2 obtained by treating the pretreated sample again with a ligand-immobilized solid phase carrier in terms of the proteins contained therein, and identifying a protein whose content is remarkably decreased in the extract 2 compared to the extract 1, 1) the problem of the solubility of subject ligand, 2) the problem of the non-specific protein-denaturing effect of the subject ligand added, and the like, which have been problematic in antagonism experiments in target search using an affinity resin, have been solved.

    摘要翻译： 在包括将通过预处理样品获得的固相载体提取物1与固定配体的固相载体和固相载体提取物2进行比较的方法中，所述固相载体提取物2通过用配体固定的固相载体再次处理预处理的样品而得到 的蛋白质，并且鉴定与提取物1相比，提取物2中的含量显着降低的蛋白质，1）主体配体的溶解度的问题，2）非特异性蛋白质变性效应的问题 已经解决了使用亲和性树脂在靶搜索中的拮抗实验中存在问题的添加的受试者配体等。

    Method of immobilizing compound on solid phase support
    8.
    发明申请
    Method of immobilizing compound on solid phase support 失效
    将化合物固定在固相载体上的方法

    公开(公告)号：US20060024845A1

    公开(公告)日：2006-02-02

    申请号：US10533237

    申请日：2003-10-31

    申请人： Akito Tanaka ,  Tomohiro Terada ,  Akira Yamazaki ,  Tsuruki Tamura ,  Hidenori Nakajima

    发明人： Akito Tanaka ,  Tomohiro Terada ,  Akira Yamazaki ,  Tsuruki Tamura ,  Hidenori Nakajima

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54353 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/289 ,  G01N2500/00 ,  Y10S436/823 ,  Y10S436/824

    摘要： The present invention provides a method of analyzing the specific interaction between a molecule to be analyzed and a molecule that specifically interacts with the former molecule on a solid phase using a molecule-immobilized solid phase support mixture prepared by binding the subject molecule to the solid phase support without specifying the binding position on the molecule side, particularly a method wherein the immobilization is conducted via a spacer introduced to the molecule without specifying the binding position on the molecule side, which method makes it possible to identify and select only a molecule that exhibits a specific interaction with a molecule to be analyzed, without an investigation of structure-activity correlation, which has conventionally been essential, and hence enables an analysis of the interaction between these molecules.

    摘要翻译： 本发明提供了一种分析待分析分子与在固相上与前一分子特异性相互作用的分子之间的特异性相互作用的方法，其中所述分子固定的固相支持混合物是通过将受试分子与固相结合而制备的 特别是一种方法，其中通过分子引入的间隔物进行固定，而不指定分子侧的结合位置，该方法使得可以仅鉴定和选择表现出分子侧的结合位置的分子， 与待分析的分子的特异性相互作用，而不具有传统上必需的结构 - 活性相关性的研究，因此能够分析这些分子之间的相互作用。

    Method of calibrating ligand specificity
    9.
    发明授权
    Method of calibrating ligand specificity 有权
    校准配体特异性的方法

    公开(公告)号：US07923211B2

    公开(公告)日：2011-04-12

    申请号：US12543801

    申请日：2009-08-19

    申请人： Akito Tanaka ,  Akira Yamazaki

    发明人： Akito Tanaka ,  Akira Yamazaki

    IPC分类号： G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/54313 ,  G01N33/54393 ,  Y10S435/973 ,  Y10T436/10 ,  Y10T436/25375

    摘要： A method to determine specificity of ligand binding includes comparing a solid phase carrier first extract obtained by pre-treating a sample with a ligand-immobilized solid phase carrier and a solid phase carrier second extract obtained by treating the pretreated sample again with a ligand-immobilized solid phase carrier in terms of the proteins contained therein, and identifying a protein whose content is remarkably decreased in the second extract compared to the first extract, in order to solve 1) the problem of the solubility of subject ligand, 2) the problem of the non-specific protein-denaturing effect of the subject ligand added, and the like, in antagonism experiments in target search using an affinity resin.

    摘要翻译： 确定配体结合特异性的方法包括比较通过预处理样品与固定配体的固相载体获得的固相载体第一提取物和通过用配体固定化处理再次处理预处理的样品获得的固相载体第二提取物 固相载体与其中所含的蛋白质相比，并鉴定出与第一提取物相比，第二提取物中其含量显着降低的蛋白质，以解决1）本发明配体的溶解度问题，2） 使用亲和树脂进行目标检测的拮抗实验中加入的受试者配体的非特异性蛋白质变性效应等。

    METHOD FOR EFFECTIVE SEARCH FOR TARGET MOLECULE
    10.
    发明申请
    METHOD FOR EFFECTIVE SEARCH FOR TARGET MOLECULE 审中-公开
    有效搜索目标分子的方法

    公开(公告)号：US20090042318A1

    公开(公告)日：2009-02-12

    申请号：US11814986

    申请日：2006-01-26

    申请人： Akito Tanaka ,  Akira Yamazaki ,  Masayuki Haramura

    发明人： Akito Tanaka ,  Akira Yamazaki ,  Masayuki Haramura

    IPC分类号： G01N33/545

    CPC分类号： G01N33/54393 ,  B01J20/3227 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3255 ,  B01J20/3285 ,  G01N33/54306

    摘要： The present invention provides a solid phase carrier capable of adsorbing a highly hydrophobic target molecule, for example, a membrane associated protein, and a solid phase carrier optimized not only for a highly hydrophobic target molecule, but also for an optionally chosen target molecule. More specifically, the present invention provides a solid phase carrier having a ligand and a capping agent immobilized thereon, with the hydrophobic property of the surface thereof adjusted to enable the binding of the target molecule to the ligand, or to increase the amount of target molecule bound to the ligand; various methods using the solid phase carrier (for example, method of concentrating, isolating, or purifying a target molecule, a method of selectively adsorbing a particular target molecule to the solid phase carrier, or a method of analyzing the interaction between ligand and target molecule therefor); a production method for the solid phase carrier; and an improvement method for a solid phase carrier having a ligand and a capping agent immobilized thereon and the like.

    摘要翻译： 本发明提供了能够吸附高度疏水的靶分子例如膜相关蛋白的固相载体，以及不仅对于高度疏水的靶分子而且优选任选地选择的靶分子优化的固相载体。 更具体地说，本发明提供一种具有固定在其上的配体和封端剂的固相载体，其表面的疏水性被调节以使靶分子与配体结合，或增加靶分子的量 与配体结合; 使用固相载体的各种方法（例如，浓缩，分离或纯化靶分子的方法，将特定靶分子选择性吸附到固相载体上的方法或分析配体与靶分子之间的相互作用的方法 为此） 一种固相载体的制备方法; 以及其上固定有配体和封端剂等的固相载体的改进方法。