-
公开(公告)号：US20240294886A1
公开(公告)日：2024-09-05
申请号：US18652554
申请日：2024-05-01
Applicant: Agilent Technologies, Inc.
Inventor： Michelle Cayouette , Jeffrey Fox , Connie Hansen , Holly Hogrefe , Weidong Wu
IPC: C12N9/12 , C12P19/34 , C12Q1/6869
CPC classification number: C12N9/1252 , C12P19/34 , C12Q1/6869 , C12Y207/07007
Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
-
公开(公告)号：US20170342483A1
公开(公告)日：2017-11-30
申请号：US15167823
申请日：2016-05-27
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Bahram Arezi , Michael Borns , Holly Hogrefe , Connie Hansen
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6869 , C12Q2521/501 , C12Q2521/507 , C12Q2525/179 , C12Q2525/191 , C12Q2535/122 , C12Q2565/514
Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
-
公开(公告)号：US12006519B2
公开(公告)日：2024-06-11
申请号：US18464955
申请日：2023-09-11
Applicant: Agilent Technologies, Inc.
Inventor： Michelle Cayouette , Jeffrey Fox , Connie Hansen , Holly Hogrefe , Weidong Wu
IPC: C12N9/12 , C12P19/34 , C12Q1/6869
CPC classification number: C12N9/1252 , C12P19/34 , C12Q1/6869 , C12Y207/07007
Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
-
公开(公告)号：US20240018490A1
公开(公告)日：2024-01-18
申请号：US18464955
申请日：2023-09-11
Applicant: Agilent Technologies, Inc.
Inventor： Michelle Cayouette , Jeffrey Fox , Connie Hansen , Holly Hogrefe , Weidong Wu
IPC: C12N9/12 , C12Q1/6869 , C12P19/34
CPC classification number: C12N9/1252 , C12Y207/07007 , C12Q1/6869 , C12P19/34
Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
-
公开(公告)号：US20200071690A1
公开(公告)日：2020-03-05
申请号：US16116147
申请日：2018-08-29
Applicant: Agilent Technologies, Inc.
Inventor： Jeffrey Carl Braman , Peter James Sheffield , Holly Hogrefe
IPC: C12N15/10 , C12N15/11 , C12N15/66 , C12Q1/6853 , C12Q1/686
Abstract: Methods and compositions are provided for amplifying a pool of oligonucleotides, such as dual guide oligonucleotide constructs comprising sequences encoding a first guide RNA segment and a sequence encoding a second guide RNA segment. An amplification mixture is formed comprising the pool of oligonucleotides, an amplification enzyme, deoxyribonucleotide triphosphates, and primers. The amplification mixture is thermocycled a sufficient number of times and under conditions to produce a library of oligonucleotide constructs. The present methods and compositions provide dual guide libraries, including libraries that are essentially free of scrambled library members.
-
Patent Agency Ranking
公开(公告)号：US11773380B1
公开(公告)日：2023-10-03
申请号：US17894854
申请日：2022-08-24
Applicant: Agilent Technologies, Inc.
Inventor： Michelle Cayouette , Jeffrey Fox , Connie Hansen , Holly Hogrefe , Weidong Wu
IPC: C12N9/12 , C12P19/34 , C12Q1/6869
CPC classification number: C12N9/1252 , C12P19/34 , C12Q1/6869 , C12Y207/07007
Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.
-
公开(公告)号：US11718847B2
公开(公告)日：2023-08-08
申请号：US16116147
申请日：2018-08-29
Applicant: Agilent Technologies, Inc.
Inventor： Jeffrey Carl Braman , Peter James Sheffield , Holly Hogrefe
CPC classification number: C12N15/1065 , C12N15/1068 , C12N15/1096 , C12N15/11 , C12N15/66 , C12Q1/686 , C12Q1/6853 , C12N2330/31 , C40B50/04 , C40B50/06 , C40B70/00
Abstract: Methods and compositions are provided for amplifying a pool of oligonucleotides, such as dual guide oligonucleotide constructs comprising sequences encoding a first guide RNA segment and a sequence encoding a second guide RNA segment. An amplification mixture is formed comprising the pool of oligonucleotides, an amplification enzyme, deoxyribonucleotide triphosphates, and primers. The amplification mixture is thermocycled a sufficient number of times and under conditions to produce a library of oligonucleotide constructs. The present methods and compositions provide dual guide libraries, including libraries that are essentially free of scrambled library members.
-
公开(公告)号：US20230126241A1
公开(公告)日：2023-04-27
申请号：US17914700
申请日：2021-02-03
Applicant: Agilent Technologies, Inc.
Inventor： Jinchun Wang , Jurgen Del Favero , Weidong Wu , Holly Hogrefe
IPC: C12Q1/686 , C12Q1/6876
Abstract: Methods and compositions are described for multi-stage primer extension reactions such as multiplex polymerase chain reactions (PCR) and reverse transcriptase PCR. Primer extension stages are performed in a closed vessel without opening the vessel between stages. The multi-stage primer extension methods and compositions utilize earlier stage primers in an earlier stage and later stage primers in a later stage, wherein the later stage primers are blocked from extension during the earlier stage. The blocked primers of the present technology comprise photocleavable blocking groups and are substantially inactive until the blocking group is cleaved by exposure to ultraviolet light. The blocked primers can be activated by ultraviolet light without opening the vessel.
-
公开(公告)号：USRE49207E1
公开(公告)日：2022-09-13
申请号：US16678868
申请日：2019-11-08
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Bahram Arezi , Michael Borns , Holly Hogrefe , Connie Hansen
IPC: C12P19/34 , C12Q1/6806 , C12Q1/6874 , C12Q1/6869
Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
-
公开(公告)号：US10308979B2
公开(公告)日：2019-06-04
申请号：US13830408
申请日：2013-03-14
Applicant: Agilent Technologies, Inc.
Inventor： Brian Jon Peter , Robert A. Ach , Zoltan Timar , Joel Myerson , Jeffrey Robert Sampson , Holly Hogrefe
IPC: C12Q1/68 , C12Q1/6813 , C12Q1/6806
Abstract: This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.
-
-
-
-
-
-
-
-
-
Search Results
11
records
(took 0.017 seconds)
