公开(公告)号：US09896472B2

公开(公告)日：2018-02-20

申请号：US14853790

申请日：2015-09-14

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J. Dellinger ,  Joel Myerson ,  Agnieszka Sierzchala ,  Geraldine F. Dellinger ,  Zoltan Timar

IPC: C07H21/02 ,  C07H21/04 ,  C07H19/067 ,  C07H13/12 ,  A61K31/7125 ,  A61K31/712 ,  C07H19/00 ,  C07H19/167 ,  C07H21/00 ,  C07H23/00 ,  C07H1/00

CPC classification number: C07H19/067 ,  A61K31/712 ,  A61K31/7125 ,  C07H1/00 ,  C07H13/12 ,  C07H19/00 ,  C07H19/167 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C07H23/00 ,  Y02P20/55

Abstract: A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2′-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2′-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.

公开(公告)号：US20160002282A1

公开(公告)日：2016-01-07

申请号：US14853790

申请日：2015-09-14

Applicant: Agilent Technologies, Inc.

Inventor： DOUGLAS J. DELLINGER ,  Joel Myerson ,  Agnieszka SIERZCHALA ,  Geraldine F. Dellinger ,  Zoltan Timar

IPC: C07H21/02 ,  C07H1/00

CPC classification number: C07H19/067 ,  A61K31/712 ,  A61K31/7125 ,  C07H1/00 ,  C07H13/12 ,  C07H19/00 ,  C07H19/167 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C07H23/00 ,  Y02P20/55

Abstract: A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2′-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2′-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.

Abstract translation: 使固体支持物结合的多核苷酸去保护的方法包括在足以使2'-保护的核糖核苷酸残基脱保护的条件下使多核苷酸与包含二胺的组合物接触的步骤。 固体支持结合的多核苷酸具有至少一个2'-保护的核糖核苷酸残基，其具有以下结构：其中BP是被保护或未被保护的杂环; R 12是选自烃基，取代的烃基，芳基和取代的芳基的保护基; X是O或S; PG是硫代氨基甲酸酯保护基。

公开(公告)号：US10597421B2

公开(公告)日：2020-03-24

申请号：US15839547

申请日：2017-12-12

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J. Dellinger ,  Joel Myerson ,  Agnieszka Sierzchala ,  Geraldine F. Dellinger ,  Zoltan Timar

IPC: C07H21/02 ,  C07H21/04 ,  C07H21/00 ,  C08L85/02 ,  C07H19/067 ,  C07H13/12 ,  A61K31/7125 ,  A61K31/712 ,  C07H19/00 ,  C07H19/167 ,  C07H23/00 ,  C07H1/00

Abstract: A method of deprotecting a solid support bound polynucleotide comprising at least one 2′-protected ribonucleotide in which a step of contacting the polynucleotide with a composition comprising a diamine is performed under conditions sufficient to deprotect and cleave the polynucleotide which remains retained on the solid support.

公开(公告)号：US20180186826A1

公开(公告)日：2018-07-05

申请号：US15839547

申请日：2017-12-12

Applicant: Agilent Technologies, Inc.

Inventor： Douglas J. Dellinger ,  Joel Myerson ,  Agnieszka Sierzchala ,  Geraldine F. Dellinger ,  Zoltan Timar

IPC: C07H19/067 ,  C07H23/00 ,  C07H21/00 ,  C07H19/167 ,  C07H13/12 ,  C07H21/04 ,  C07H1/00 ,  A61K31/712 ,  A61K31/7125 ,  C07H21/02 ,  C07H19/00

Abstract: A method of deprotecting a solid support bound polynucleotide comprising at least one 2′-protected ribonucleotide in which a step of contacting the polynucleotide with a composition comprising a diamine is performed under conditions sufficient to deprotect and cleave the polynucleotide which remains retained on the solid support.

公开(公告)号：US10308979B2

公开(公告)日：2019-06-04

申请号：US13830408

申请日：2013-03-14

Applicant: Agilent Technologies, Inc.

Inventor： Brian Jon Peter ,  Robert A. Ach ,  Zoltan Timar ,  Joel Myerson ,  Jeffrey Robert Sampson ,  Holly Hogrefe

IPC: C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6806

Abstract: This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.