公开(公告)号：US07686907B1

公开(公告)日：2010-03-30

申请号：US11346398

申请日：2006-02-01

Applicant: Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

Inventor： Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

IPC: B32B17/00 ,  B32B17/10 ,  B32B37/00 ,  G02C7/00 ,  B29C65/00 ,  B29C53/82 ,  G01N21/00 ,  G01N31/22 ,  G01N33/00 ,  G01N15/06 ,  B01L11/00 ,  C03C15/00 ,  C03C25/68 ,  C03C27/00 ,  B32B38/04 ,  C02F1/40 ,  C02F11/00 ,  C25B9/00 ,  C25B11/00 ,  C25B13/00 ,  G01N27/00

CPC classification number: B32B38/06 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0887 ,  B29C65/4895 ,  B29C65/76 ,  B29C66/0224 ,  B29C66/0246 ,  B29C66/026 ,  B29C66/032 ,  B29C66/1122 ,  B29C66/3242 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/73117 ,  B29K2021/00 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2067/043 ,  B29K2069/00 ,  B29K2083/00 ,  B29L2031/756 ,  B32B37/0023 ,  B32B2309/027 ,  B32B2323/16 ,  B32B2333/12 ,  B32B2367/00 ,  B32B2369/00 ,  B81B2201/058 ,  B81C1/00071 ,  B81C2201/0108 ,  B81C2203/038 ,  G01N21/05 ,  G01N27/44791 ,  G01N2021/0346 ,  G01N2021/6439 ,  B29K2067/003

Abstract: A microchip with capillaries and method for making same is described. A sacrificial material fills microchannels formed in a polymeric substrate, the filled microchannels are covered by a top cover to form filed capillaries, and the sacrificial material is removed to form the microcapillaries. The sacrificial material fills the microchannels as a liquid whereupon it becomes solid in the microchannels, and is liquefied after the top cover is applied and affixed to remove the sacrificial material. The top cover may be solvent sealed on the substrate and of the same or different material as the substrate. The top cover may also be an in situ applied semipermeable membrane.

Abstract translation: 描述了具有毛细管的微芯片及其制造方法。 牺牲材料填充形成在聚合物基材中的微通道，填充的微通道被顶盖覆盖以形成毛细管毛细管，并且去除牺牲材料以形成微毛细管。 牺牲材料作为液体填充微通道，随后在微通道中变成固体，并且在施加顶盖并固定以去除牺牲材料之后液化。 顶盖可以在基材上和与基材相同或不同的材料上进行溶剂密封。 顶盖也可以是原位应用的半透膜。

公开(公告)号：US08101037B1

公开(公告)日：2012-01-24

申请号：US12724404

申请日：2010-03-15

Applicant: Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

Inventor： Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

IPC: B32B17/00 ,  B32B17/10 ,  C03C27/00 ,  C03C15/00 ,  C03C25/68 ,  G02C7/00 ,  B29C53/82 ,  G01N21/00 ,  G01N31/22 ,  G01N33/00 ,  G01N15/06

CPC classification number: B32B38/06 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0887 ,  B29C65/4895 ,  B29C65/76 ,  B29C66/0224 ,  B29C66/0246 ,  B29C66/026 ,  B29C66/032 ,  B29C66/1122 ,  B29C66/3242 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/73117 ,  B29K2021/00 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2067/043 ,  B29K2069/00 ,  B29K2083/00 ,  B29L2031/756 ,  B32B37/0023 ,  B32B2309/027 ,  B32B2323/16 ,  B32B2333/12 ,  B32B2367/00 ,  B32B2369/00 ,  B81B2201/058 ,  B81C1/00071 ,  B81C2201/0108 ,  B81C2203/038 ,  G01N21/05 ,  G01N27/44791 ,  G01N2021/0346 ,  G01N2021/6439 ,  B29K2067/003

Abstract: A microchip with capillaries and method for making same is described. A sacrificial material fills microchannels formed in a polymeric substrate, the filled microchannels are covered by a top cover to form filed capillaries, and the sacrificial material is removed to form the microcapillaries. The sacrificial material fills the microchannels as a liquid whereupon it becomes solid in the microchannels, and is liquefied after the top cover is applied and affixed to remove the sacrificial material. The top cover may be solvent sealed on the substrate and of the same or different material as the substrate. The top cover may also be an in situ applied semipermeable membrane.

Abstract translation: 描述了具有毛细管的微芯片及其制造方法。 牺牲材料填充形成在聚合物基材中的微通道，填充的微通道被顶盖覆盖以形成毛细管毛细管，并且去除牺牲材料以形成微毛细管。 牺牲材料作为液体填充微通道，随后在微通道中变成固体，并且在施加顶盖并固定以去除牺牲材料之后液化。 顶盖可以在基材上和与基材相同或不同的材料上进行溶剂密封。 顶盖也可以是原位应用的半透膜。

公开(公告)号：US20140308754A1

公开(公告)日：2014-10-16

申请号：US14170941

申请日：2014-02-03

Applicant: Adam T. Woolley ,  Debolina Chatterjee ,  Danielle Scarlet Mansfield

Inventor： Adam T. Woolley ,  Debolina Chatterjee ,  Danielle Scarlet Mansfield

IPC: G01N21/78

CPC classification number: G01N21/78 ,  B01L3/502746 ,  B01L2400/0672 ,  B01L2400/084 ,  G01N33/54366

Abstract: A system for detecting concentration of a target in a solution where sample fluid is passed into a microchannel with wall coated with the receptor that reacts and crosslinks with the target to constrict the channel and slow or stop sample flow through the microchannel. Concentration of the target is determined by measuring length of the sample filled channel.

Abstract translation: 一种用于检测溶液中靶的浓度的系统，其中样品流体进入具有与靶反应并与靶交联的受体的壁的微通道中，以收缩通道并减缓或停止样品流过微通道。 通过测量样品填充通道的长度来确定靶的浓度。

公开(公告)号：US6045676A

公开(公告)日：2000-04-04

申请号：US916557

申请日：1997-08-22

Applicant: Richard A. Mathies ,  Alexander N. Glazer ,  Adam T. Woolley ,  Kaigin Lao

Inventor： Richard A. Mathies ,  Alexander N. Glazer ,  Adam T. Woolley ,  Kaigin Lao

IPC: G01N27/447 ,  G01N30/60 ,  G01N37/00 ,  G01N27/26

CPC classification number: G01N27/4473 ,  G01N27/44791 ,  G01N30/6095

Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.

Abstract translation: 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。

公开(公告)号：US11369945B2

公开(公告)日：2022-06-28

申请号：US16355692

申请日：2019-03-15

Applicant: Adam T. Woolley ,  Radim Knob

Inventor： Adam T. Woolley ,  Radim Knob

IPC: B01J20/26 ,  B01J20/28 ,  G01N30/60 ,  G01N30/52 ,  G01N21/64

Abstract: A method and microfluidic device with a porous polymer monolith in a channel of the device with capture affinity element (such as an oligonucleotide complementary to a DNA target from the KPC antibiotic resistance gene) on the monolith surface.

公开(公告)号：US5906723A

公开(公告)日：1999-05-25

申请号：US703394

申请日：1996-08-26

Applicant: Richard A. Mathies ,  Alexander N. Glazer ,  Kaiqin Lao ,  Adam T. Woolley

Inventor： Richard A. Mathies ,  Alexander N. Glazer ,  Kaiqin Lao ,  Adam T. Woolley

IPC: G01N27/447 ,  G01N30/60 ,  G01N27/26

CPC classification number: G01N27/44791 ,  G01N27/4473 ,  G01N30/6095

Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.

Abstract translation: 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。

公开(公告)号：US20110070664A1

公开(公告)日：2011-03-24

申请号：US12857371

申请日：2010-08-16

Applicant: Adam T. Woolley ,  Weichun Yang ,  Xiuhua Sun

Inventor： Adam T. Woolley ,  Weichun Yang ,  Xiuhua Sun

IPC: G01N33/543 ,  G01N30/96 ,  G01N31/00 ,  G01N21/00 ,  G01N21/76 ,  G01N1/18 ,  G01N27/447

CPC classification number: G01N33/54386 ,  G01N21/6428 ,  G01N27/44726 ,  G01N27/44791 ,  G01N33/543 ,  G01N33/54366 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/101666 ,  Y10T436/255

Abstract: Apparatus and method for determining concentration of one or more target compounds in a sample solution containing one or more nontarget compounds that uses an affinity column to immobilize the target compounds. The target compounds are eluted and passed through a separation/detection system to determine quantitative measure of concentration for each of the target compound.

Abstract translation: 用于测定含有一种或多种使用亲和柱固定目标化合物的非目标化合物的样品溶液中一种或多种靶化合物的浓度的装置和方法。 将目标化合物洗脱并通过分离/检测系统以确定每种目标化合物的浓度的定量测量。

公开(公告)号：US6159742A

公开(公告)日：2000-12-12

申请号：US326100

申请日：1999-06-04

Applicant: Charles M. Lieber ,  Stanislaus S. Wong ,  Adam T. Woolley ,  Ernesto Joselevich

Inventor： Charles M. Lieber ,  Stanislaus S. Wong ,  Adam T. Woolley ,  Ernesto Joselevich

IPC: G01N21/00 ,  G01N33/53 ,  G01N33/544 ,  G01Q60/00 ,  G01Q70/14 ,  G01Q70/18

CPC classification number: G01Q70/12 ,  G01Q70/18 ,  Y10S977/745 ,  Y10S977/86 ,  Y10S977/875 ,  Y10S977/876 ,  Y10S977/881

Abstract: A carbon-based tip for scanning probe microscopy. The tip used in microscopy to reveal chemical characteristics of a sample includes a structure of the formula:X--(L--M).sub.nin which n is 1 to 100, X is a carbon-based nanotube, L is a linking group bonded at an end of the carbon-based nanotube, and M is a molecular probe bonded to the linking group.

Abstract translation: 用于扫描探针显微镜的碳基尖端。 显微镜用于显示样品的化学特性的尖端包括下式的结构：其中n为1至100的X-（LM）n，X为碳基纳米管，L为在末端键合的连接基团 碳基纳米管，M是与连接基团键合的分子探针。

公开(公告)号：US6143152A

公开(公告)日：2000-11-07

申请号：US965738

申请日：1997-11-07

Applicant: Peter C. Simpson ,  Richard A. Mathies ,  Adam T. Woolley

Inventor： Peter C. Simpson ,  Richard A. Mathies ,  Adam T. Woolley

IPC: C08F2/58 ,  G01N27/447 ,  G01N37/00 ,  G01N27/26

CPC classification number: G01N27/44791 ,  G01N27/44782 ,  Y10S366/03

Abstract: A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.

Abstract translation: 毛细管阵列电泳（CAE）微板，其具有连接到板上的样品储存器阵列的分离通道阵列。 样品池被组织成一个或多个样品注射器。 提供一个或多个废物储存器以从每个样品注射器中的储存器收集废物。 此外，阴极储存器也与一个或多个分离通道复用。 为了完成电路径，在微板上还设置有一些或所有分离通道共用的阳极储存器。 此外，通道布局保持从阳极到每个阴极的距离近似恒定。

公开(公告)号：US20200078764A1

公开(公告)日：2020-03-12

申请号：US16355692

申请日：2019-03-15

Applicant: Adam T. Woolley ,  Radim Knob

Inventor： Adam T. Woolley ,  Radim Knob

IPC: B01J20/28 ,  B01J20/26 ,  G01N30/60 ,  G01N21/64

Abstract: A method and microfluidic device with a porous polymer monolith in a channel of the device with capture affinity element (such as an oligonucleotide complementary to a DNA target from the KPC antibiotic resistance gene) on the monolith surface.