公开(公告)号：US11369945B2

公开(公告)日：2022-06-28

申请号：US16355692

申请日：2019-03-15

Applicant: Adam T. Woolley ,  Radim Knob

Inventor： Adam T. Woolley ,  Radim Knob

IPC: B01J20/26 ,  B01J20/28 ,  G01N30/60 ,  G01N30/52 ,  G01N21/64

Abstract: A method and microfluidic device with a porous polymer monolith in a channel of the device with capture affinity element (such as an oligonucleotide complementary to a DNA target from the KPC antibiotic resistance gene) on the monolith surface.

公开(公告)号：US07686907B1

公开(公告)日：2010-03-30

申请号：US11346398

申请日：2006-02-01

Applicant: Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

Inventor： Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

IPC: B32B17/00 ,  B32B17/10 ,  B32B37/00 ,  G02C7/00 ,  B29C65/00 ,  B29C53/82 ,  G01N21/00 ,  G01N31/22 ,  G01N33/00 ,  G01N15/06 ,  B01L11/00 ,  C03C15/00 ,  C03C25/68 ,  C03C27/00 ,  B32B38/04 ,  C02F1/40 ,  C02F11/00 ,  C25B9/00 ,  C25B11/00 ,  C25B13/00 ,  G01N27/00

CPC classification number: B32B38/06 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0887 ,  B29C65/4895 ,  B29C65/76 ,  B29C66/0224 ,  B29C66/0246 ,  B29C66/026 ,  B29C66/032 ,  B29C66/1122 ,  B29C66/3242 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/73117 ,  B29K2021/00 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2067/043 ,  B29K2069/00 ,  B29K2083/00 ,  B29L2031/756 ,  B32B37/0023 ,  B32B2309/027 ,  B32B2323/16 ,  B32B2333/12 ,  B32B2367/00 ,  B32B2369/00 ,  B81B2201/058 ,  B81C1/00071 ,  B81C2201/0108 ,  B81C2203/038 ,  G01N21/05 ,  G01N27/44791 ,  G01N2021/0346 ,  G01N2021/6439 ,  B29K2067/003

Abstract: A microchip with capillaries and method for making same is described. A sacrificial material fills microchannels formed in a polymeric substrate, the filled microchannels are covered by a top cover to form filed capillaries, and the sacrificial material is removed to form the microcapillaries. The sacrificial material fills the microchannels as a liquid whereupon it becomes solid in the microchannels, and is liquefied after the top cover is applied and affixed to remove the sacrificial material. The top cover may be solvent sealed on the substrate and of the same or different material as the substrate. The top cover may also be an in situ applied semipermeable membrane.

Abstract translation: 描述了具有毛细管的微芯片及其制造方法。 牺牲材料填充形成在聚合物基材中的微通道，填充的微通道被顶盖覆盖以形成毛细管毛细管，并且去除牺牲材料以形成微毛细管。 牺牲材料作为液体填充微通道，随后在微通道中变成固体，并且在施加顶盖并固定以去除牺牲材料之后液化。 顶盖可以在基材上和与基材相同或不同的材料上进行溶剂密封。 顶盖也可以是原位应用的半透膜。

公开(公告)号：US5906723A

公开(公告)日：1999-05-25

申请号：US703394

申请日：1996-08-26

Applicant: Richard A. Mathies ,  Alexander N. Glazer ,  Kaiqin Lao ,  Adam T. Woolley

Inventor： Richard A. Mathies ,  Alexander N. Glazer ,  Kaiqin Lao ,  Adam T. Woolley

IPC: G01N27/447 ,  G01N30/60 ,  G01N27/26

CPC classification number: G01N27/44791 ,  G01N27/4473 ,  G01N30/6095

Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.

Abstract translation: 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。

公开(公告)号：US20220334034A1

公开(公告)日：2022-10-20

申请号：US17658442

申请日：2022-04-07

Applicant: Adam T. WOOLLEY ,  Anna V. BICKHAM ,  Gregory P. NORDIN

Inventor： Adam T. WOOLLEY ,  Anna V. BICKHAM ,  Gregory P. NORDIN

IPC: G01N1/40 ,  G01N33/58 ,  B33Y80/00

Abstract: Miniaturized solid-phase extraction (SPE) systems and processes are disclosed. The systems and methods enable preconcentration methods for sample preparation that can be performed on a variety of specimens. The miniaturization of SPE system is accomplished with 3D-printed microfluidic device for fast and simple extraction of analytes.

公开(公告)号：US20140308754A1

公开(公告)日：2014-10-16

申请号：US14170941

申请日：2014-02-03

Applicant: Adam T. Woolley ,  Debolina Chatterjee ,  Danielle Scarlet Mansfield

Inventor： Adam T. Woolley ,  Debolina Chatterjee ,  Danielle Scarlet Mansfield

IPC: G01N21/78

CPC classification number: G01N21/78 ,  B01L3/502746 ,  B01L2400/0672 ,  B01L2400/084 ,  G01N33/54366

Abstract: A system for detecting concentration of a target in a solution where sample fluid is passed into a microchannel with wall coated with the receptor that reacts and crosslinks with the target to constrict the channel and slow or stop sample flow through the microchannel. Concentration of the target is determined by measuring length of the sample filled channel.

Abstract translation: 一种用于检测溶液中靶的浓度的系统，其中样品流体进入具有与靶反应并与靶交联的受体的壁的微通道中，以收缩通道并减缓或停止样品流过微通道。 通过测量样品填充通道的长度来确定靶的浓度。

公开(公告)号：US6045676A

公开(公告)日：2000-04-04

申请号：US916557

申请日：1997-08-22

Applicant: Richard A. Mathies ,  Alexander N. Glazer ,  Adam T. Woolley ,  Kaigin Lao

Inventor： Richard A. Mathies ,  Alexander N. Glazer ,  Adam T. Woolley ,  Kaigin Lao

IPC: G01N27/447 ,  G01N30/60 ,  G01N37/00 ,  G01N27/26

CPC classification number: G01N27/4473 ,  G01N27/44791 ,  G01N30/6095

Abstract: A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.

Abstract translation: 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。

公开(公告)号：US08703276B2

公开(公告)日：2014-04-22

申请号：US12184131

申请日：2008-07-31

Applicant: Héctor Alejandro Becerril García ,  Adam T. Woolley

Inventor： Héctor Alejandro Becerril García ,  Adam T. Woolley

IPC: B32B3/00 ,  C12Q1/68 ,  C12M1/00 ,  G01N33/00

CPC classification number: H01L21/3086 ,  B82Y10/00 ,  C23C14/042 ,  C23C14/225

Abstract: Nanostructures on substrates include one or more nanofeatures having unscathed walls and width dimensions of forty-five nm or less. The nanofeatures may include at least one of a nanotrench, nanocapillary, nano-chemical pattern, and nanowire. The nanostructures may include a nano object with a pattern of nano elements. A nano system may include at least one nano system device, which may include at least one nanofeature. A method of forming nanofeatures on substrates includes placing a nano-templating element on the substrate. A masking material is deposited at an acute angle to form shadow gaps on shadowed regions of the substrate. The nano-templating element, the angle, and other factors may be selected to form shadow gaps having width dimensions less than 10 nm. The substrate may be chemically modified in the areas corresponding to the shadow gaps to create nanofeatures with unscathed walls having width dimensions of less than 10 nm.

Abstract translation: 衬底上的纳米结构包括一个或多个具有无损壁的纳米尺寸和宽度尺寸为45nm或更小的纳米结构。 纳米尺度可以包括纳米管，纳米毛细管，纳米化学图案和纳米线中的至少一个。 纳米结构可以包括具有纳米元素图案的纳米物体。 纳米系统可以包括至少一个纳米系统器件，其可以包括至少一个纳米级。 在衬底上形成纳米尺度的方法包括将纳米模板元件放置在衬底上。 以锐角沉积掩模材料，以在衬底的阴影区域上形成阴影间隙。 可以选择纳米模板元件，角度和其它因素以形成宽度尺寸小于10nm的阴影间隙。 可以在对应于阴影间隙的区域中对衬底进行化学修饰，以产生具有宽度尺寸小于10nm的无损壁的纳米尺度。

公开(公告)号：US08101037B1

公开(公告)日：2012-01-24

申请号：US12724404

申请日：2010-03-15

Applicant: Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

Inventor： Adam T. Woolley ,  Ryan T. Kelly ,  Melissa Draper Fisk

IPC: B32B17/00 ,  B32B17/10 ,  C03C27/00 ,  C03C15/00 ,  C03C25/68 ,  G02C7/00 ,  B29C53/82 ,  G01N21/00 ,  G01N31/22 ,  G01N33/00 ,  G01N15/06

CPC classification number: B32B38/06 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0887 ,  B29C65/4895 ,  B29C65/76 ,  B29C66/0224 ,  B29C66/0246 ,  B29C66/026 ,  B29C66/032 ,  B29C66/1122 ,  B29C66/3242 ,  B29C66/53461 ,  B29C66/71 ,  B29C66/73117 ,  B29K2021/00 ,  B29K2033/12 ,  B29K2067/00 ,  B29K2067/043 ,  B29K2069/00 ,  B29K2083/00 ,  B29L2031/756 ,  B32B37/0023 ,  B32B2309/027 ,  B32B2323/16 ,  B32B2333/12 ,  B32B2367/00 ,  B32B2369/00 ,  B81B2201/058 ,  B81C1/00071 ,  B81C2201/0108 ,  B81C2203/038 ,  G01N21/05 ,  G01N27/44791 ,  G01N2021/0346 ,  G01N2021/6439 ,  B29K2067/003

Abstract: A microchip with capillaries and method for making same is described. A sacrificial material fills microchannels formed in a polymeric substrate, the filled microchannels are covered by a top cover to form filed capillaries, and the sacrificial material is removed to form the microcapillaries. The sacrificial material fills the microchannels as a liquid whereupon it becomes solid in the microchannels, and is liquefied after the top cover is applied and affixed to remove the sacrificial material. The top cover may be solvent sealed on the substrate and of the same or different material as the substrate. The top cover may also be an in situ applied semipermeable membrane.

Abstract translation: 描述了具有毛细管的微芯片及其制造方法。 牺牲材料填充形成在聚合物基材中的微通道，填充的微通道被顶盖覆盖以形成毛细管毛细管，并且去除牺牲材料以形成微毛细管。 牺牲材料作为液体填充微通道，随后在微通道中变成固体，并且在施加顶盖并固定以去除牺牲材料之后液化。 顶盖可以在基材上和与基材相同或不同的材料上进行溶剂密封。 顶盖也可以是原位应用的半透膜。

公开(公告)号：US20090041590A1

公开(公告)日：2009-02-12

申请号：US12186391

申请日：2008-08-05

Applicant: Hernan V. Fuetes ,  Adam T. Woolley

Inventor： Hernan V. Fuetes ,  Adam T. Woolley

IPC: F04B37/02

CPC classification number: F04B19/006

Abstract: Rapid microchip LC analysis with integrated electrolysis-based pumping is achieved with a monolithic microfluidic chip. A pressure-balanced sample injection approach allows introduction of pL-range sample volumes without valves or other components that are difficult to integrate in microdevices. The approach also eliminates dead volume between injection and separation. On-chip LC separation of amino acids with elution times of

Abstract translation: 使用单片微流控芯片实现了集成电解泵浦的快速微芯片LC分析。 压力平衡的样品注射方法允许引入pL范围的样品体积，而不需要阀门或难以集成到微型设备中的其他组件。 该方法还消除了注射和分离之间的死体积。 提供氨基酸的片上LC分离，洗脱时间<40秒，效率好（理论值3350）。

公开(公告)号：US06749734B1

公开(公告)日：2004-06-15

申请号：US09649272

申请日：2000-08-28

Applicant: Peter C. Simpson ,  Richard A. Mathies ,  Adam T. Woolley

Inventor： Peter C. Simpson ,  Richard A. Mathies ,  Adam T. Woolley

IPC: B01D5702

CPC classification number: G01N27/44791 ,  G01N27/44782 ,  Y10S366/03

Abstract: A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.

Abstract translation: 毛细管阵列电泳（CAE）微板，其具有连接到板上的样品储存器阵列的分离通道阵列。 样品池被组织成一个或多个样品注射器。 提供一个或多个废物储存器以从每个样品注射器中的储存器收集废物。 此外，阴极储存器也与一个或多个分离通道复用。 为了完成电路径，在微板上还提供了一些或所有分离通道共同的阳极储存器。 此外，通道布局保持从阳极到每个阴极的距离近似恒定。