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    Use of cross-protection to identify novel vaccine candidates for infectious agents
    2.
    发明申请
    Use of cross-protection to identify novel vaccine candidates for infectious agents 失效

    公开(公告)号：US20080176270A1

    公开(公告)日：2008-07-24

    申请号：US11979173

    申请日：2007-10-31

    申请人： Christopher Sikora ,  Bradley J. Berger ,  John W. Cherwonogrodzky

    发明人： Christopher Sikora ,  Bradley J. Berger ,  John W. Cherwonogrodzky

    IPC分类号： C12Q1/02

    CPC分类号： A61K39/02 ,  A61K2039/52 ,  C07K14/195 ,  G01N2469/20 ,  Y02A50/405

    摘要： This invention discloses methods for identifying Francisella tularensis vaccine candidates. It enables identification of novel vaccine candidates and quality assurance for vaccine batches, assessment of protection in vaccinates and identification of the infecting agent in vaccinates. Mice were first vaccinated with Brucella abortus O-polysaccharide (OPS) vaccine. These animals were then given 10 LD50s of F. tularensis live vaccine strain (LVS). Sixty percent (60%) of the vaccinated mice survived the multiple lethal doses. Sera were collected from these surviving mice and the antibodies were used to probe supernatant and cell lysates of live F. tularensis LVS cultures. Several F. tularensis components were identified only by the noted “survivor” antisera. Of these identified proteins, enzyme digestions and chemical oxidation suggest post-translational modifications of some proteins e.g. a 52 kDa glycoprotein, a 45 kDa lipoprotein and a 19 kDa nucleoprotein. The 52 kDa component caused nitrous oxide induction in tissue cultures at low concentrations, cell death at high concentrations. Vaccination with this gave partial protection while addition of other components acted synergistically to give enhanced protection from 250 LD50s of F. tularensis LVS.

    Bacterial and synthetic polysaccharide immunomodulators that enhance general immunity
    4.
    发明授权
    Bacterial and synthetic polysaccharide immunomodulators that enhance general immunity 失效
    细菌和合成多糖免疫调节剂，增强一般免疫力

    公开(公告)号：US06444210B1

    公开(公告)日：2002-09-03

    申请号：US08882398

    申请日：1997-06-25

    申请人： Bill Kournikakis ,  Maureen L. Simpson ,  John W. Cherwonogrodzky

    发明人： Bill Kournikakis ,  Maureen L. Simpson ,  John W. Cherwonogrodzky

    IPC分类号： A61K3902

    CPC分类号： A61K39/0208 ,  A61K39/025 ,  A61K39/098 ,  A61K2039/55583

    摘要： The present invention relates to the effects of bacterial polysaccharides on cell mediated immunity in animals. Polysaccharides of the present invention comprise the outer polysaccharides (OPS) located on bacterial cell membranes and other polysaccharides (e.g. “Poly B”) either secreted or contained within the periplasmic space. These polysaccharides have been found to enhance the general or cell mediated immunity of animals to various diseases. The invention provides for the use of such polysaccharides in preventing and treating various infections as well as in treating carcinomas. The invention also provides for synthetic polysaccharides having the same immuno-modulating effect as the bacterial polysaccharides.

    摘要翻译： 本发明涉及细菌多糖对动物细胞介导的免疫的作用。 本发明的多糖包含位于细菌细胞膜上的外多糖（OPS）和在周质空间内分泌或包含的其它多糖（例如“Poly B”）。 已经发现这些多糖增强动物对各种疾病的一般或细胞介导的免疫力。 本发明提供了这种多糖在预防和治疗各种感染以及治疗癌症中的用途。 本发明还提供具有与细菌多糖相同的免疫调节作用的合成多糖。

    Combination vaccine for enhancing immunity against brucellosis
    5.
    发明授权
    Combination vaccine for enhancing immunity against brucellosis 失效

    公开(公告)号：US06582699B2

    公开(公告)日：2003-06-24

    申请号：US09735534

    申请日：2000-12-14

    申请人： John W. Cherwonogrodzky

    发明人： John W. Cherwonogrodzky

    IPC分类号： A61K3900

    CPC分类号： C07K16/1221 ,  A61K39/00 ,  A61K39/098 ,  A61K2039/505

    摘要： A vaccine comprising a combination of Brucella “A” and “M” outer-polysaccharides (OPSs) and “R” protein antigens for enhancing immunity against brucellosis is disclosed. The OPS may be obtained from different strains or species of Brucellae (i.e. combining OPS extracted from different bacteria expressing “A” or “M” OPS, or combining OPS and OPS-protein complexes extracted from different bacteria). The OPS or OPS-protein complexes may also be obtained from a single strain expressing more than one OPS (e.g. from B. suis strain 145 which expresses “A”, “M” and possibly other OPSs). The vaccine according to the present invention overcomes the limitation of previously discovered B. abortus “A” OPS which only protects against species and strains of Brucella that had “A” OPS but not against others with different OPS.

    Method of detecting a pathogen using a virus
    6.
    发明授权
    Method of detecting a pathogen using a virus 失效
    使用病毒检测病原体的方法

    公开(公告)号：US06436652B1

    公开(公告)日：2002-08-20

    申请号：US09376288

    申请日：1999-08-18

    申请人： John W. Cherwonogrodzky ,  Kamil Lotfali

    发明人： John W. Cherwonogrodzky ,  Kamil Lotfali

    IPC分类号： G01N33554

    CPC分类号： C12Q1/701 ,  C12Q1/04 ,  C12Q1/689 ,  C12Q2563/125 ,  G01N2333/23

    摘要： A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).

    摘要翻译： 与酶连接的噬菌体可以替代用于检测样品中细菌存在的系统中的抗体。 特别是Brucella abortus（一种导致牛中布鲁氏杆菌病的病原体）可以使用Brucella噬菌体进行病毒检测，用于与噬菌体连接的酶的脲酶，作为偶联剂的间马来酰亚胺基苯甲酰基-N-羟基琥珀酰亚胺酯，用Brucella免疫的小鼠的血清 用于检测抗体的噬菌体，缀合有针对指示剂的抗小鼠绵羊抗体的脲酶和具有溴甲酚紫作为底物的脲。 这些材料可以用于间接（三明治）或直接酶联的病毒检测（ELVirA）。

    Method of detecting a pathogen using a virus
    7.
    发明授权
    Method of detecting a pathogen using a virus 失效

    公开(公告)号：US06355445B2

    公开(公告)日：2002-03-12

    申请号：US08514096

    申请日：1995-08-11

    申请人： John W. Cherwonogrodzky ,  Kamil Lotfali

    发明人： John W. Cherwonogrodzky ,  Kamil Lotfali

    IPC分类号： G01N33554

    CPC分类号： C12Q1/701 ,  C12Q1/04 ,  C12Q1/689 ,  C12Q2563/125 ,  G01N2333/23

    摘要： A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).

    Polysaccharide vaccine to enhance immunity against brucellosis
    9.
    发明授权
    Polysaccharide vaccine to enhance immunity against brucellosis 失效
    多糖疫苗增强免疫布鲁氏杆菌病

    公开(公告)号：US5951987A

    公开(公告)日：1999-09-14

    申请号：US672063

    申请日：1996-09-16

    申请人： John W. Cherwonogrodzky ,  Jonathan P. Wong ,  Vincent L. Di Ninno

    发明人： John W. Cherwonogrodzky ,  Jonathan P. Wong ,  Vincent L. Di Ninno

    IPC分类号： A61K39/02 ,  A61K39/10 ,  A61P31/04

    CPC分类号： A61K39/02

    摘要： A vaccine comprising purified outer-polysaccharide (OPS) is effective for protection against brucellosis. The vaccine is derived from Brucella or a variety of cross reactive bacteria. The vaccine can be administered by different routes (intramuscularly, subcutaneously, intraperitoneally, orally). The vaccine is effective in protecting against other infectious bacteria, aside from Brucella. It is likely that the vaccine can be given after infection to reduce illness.

    摘要翻译： 包含纯化的外多糖（OPS）的疫苗有效地防止布鲁氏菌病。 该疫苗来自布鲁杆菌或各种交叉反应性细菌。 疫苗可以通过不同的途径（肌内，皮下，腹膜内，口服）施用。 除了布鲁杆菌外，该疫苗有效地防止其他感染性细菌。 疫苗可能在感染后给予，以减少疾病。