公开(公告)号：US06835382B2

公开(公告)日：2004-12-28

申请号：US09261894

申请日：1999-03-03

申请人： Robert E. Humphreys ,  Sharlene Adams ,  Minzhen Xu

发明人： Robert E. Humphreys ,  Sharlene Adams ,  Minzhen Xu

IPC分类号： A61K3900

CPC分类号： C07K5/0812 ,  A61K38/00 ,  C07K5/1016 ,  C07K7/08 ,  C07K14/70539

摘要： Disclosed is a class of compounds referred to herein as effector compounds. Effector compounds are useful in connection with the modulation of an immune response. Modulation refers to the ability of the effector compounds of the present invention to either enhance (antigen supercharging) or inhibit (immunosuppressant activities) antigen presentation, depending upon the nature of the particular effector compound and the therapeutic context. Effector compounds include peptides, modified peptides and peptidomimetics. Also disclosed are methods for modulating presentation of an MHC class II restricted antigenic peptide to a T cell. Also disclosed are effector compounds demonstrated to act specifically on a human MHC class II allele. Also disclosed is a second class of compounds, referred to herein as immunomodulatory organic compounds. Such compounds are identified by a method which includes the following steps: providing a first complex comprising an MHC class II molecule to which an antigenic peptide has been bound; contacting the first complex with mammalian Ii key peptide LRMKLPKPPKPVSKMR (SEQ ID NO:1) (or modifications thereof including peptidomimetics), thereby forming a second complex; and screening organic molecules for compounds which bind to the second complex but not to the first complex, and which exhibit immunomodulatory activity. Compounds identified in this manner can be used to modulate an immune response in a mammal.

公开(公告)号：US06811781B2

公开(公告)日：2004-11-02

申请号：US10442173

申请日：2003-05-21

申请人： Ansgar W. Lohse

发明人： Ansgar W. Lohse

IPC分类号： A61K3900

CPC分类号： G01N33/564 ,  A61K38/00 ,  C07K14/4713 ,  C07K2319/00 ,  G01N33/5767

摘要： A composition and method for the diagnosis of autoimmune hepatitis. The composition, which contains SLA antigens detects soluble liver antigen (SLA) auto-antibodies, which occur in sera of patients suffering from chronic hepatitis.

摘要翻译： 用于诊断自身免疫性肝炎的组合物和方法。 含有SLA抗原的组合检测可溶性肝脏抗原（SLA）自身抗体，其发生在患有慢性肝炎的患者的血清中。

公开(公告)号：US06790446B2

公开(公告)日：2004-09-14

申请号：US10192419

申请日：2002-07-10

申请人： Antonius Arnoldus Christiaan Jacobs ,  Johannes Franciscus van den Bosch ,  Petrus Johannes Maria Nuijten

发明人： Antonius Arnoldus Christiaan Jacobs ,  Johannes Franciscus van den Bosch ,  Petrus Johannes Maria Nuijten

IPC分类号： A61K3900

CPC分类号： A61K39/105 ,  A61K2039/505 ,  A61K2039/5252 ,  C07K14/205 ,  C07K16/121 ,  Y02A50/47 ,  Y10S424/826

摘要： The present invention relates to vaccines comprising antiserum raised against a flagellaless Campylobacter strain for the prevention of Campylobacter colonization in animals. The invention also relates to antigenic Campylobacter proteins visible in a Western blot of Campylobacter jejuni protein after incubation of said Western blot with antibodies against a flagellaless mutant of Campylobacter jejuni and not visible after incubation of said blot with antibodies against wild type Campylobacter jejuni, and to their use in vaccines and the manufacturing thereof. The invention further relates to vaccines comprising such proteins and antibodies against such proteins. The invention further relates to the use of such Campylobacter proteins and to antiserum and antibodies raised against Campylobacter antigens for the preparation of vaccines. Finally, the invention relates to methods for the preparation of such vaccines.

摘要翻译： 本发明涉及包含抗鞭毛弯曲杆菌菌株产生的用于预防动物弯曲杆菌定植的抗血清的疫苗。 本发明还涉及在所述蛋白质印迹与抗空肠弯曲杆菌的无鞭毛虫突变体的抗体孵育后在空肠弯曲杆菌蛋白质的Western印迹中可见的抗原性弯曲杆菌蛋白质，并且在用野生型空肠弯曲杆菌抗体孵育后不可见， 它们在疫苗中的应用及其制造。 本发明还涉及包含这种蛋白质和针对这种蛋白质的抗体的疫苗。 本发明还涉及这种弯曲杆菌蛋白质的用途以及抗弯曲杆菌抗原产生的用于制备疫苗的抗血清和抗体。 最后，本发明涉及制备这种疫苗的方法。

公开(公告)号：US06790445B1

公开(公告)日：2004-09-14

申请号：US09019764

申请日：1998-02-06

申请人： Assunta S. Ng ,  Ralph J. Mancinelli ,  John P. Hennessey

发明人： Assunta S. Ng ,  Ralph J. Mancinelli ,  John P. Hennessey

IPC分类号： A61K3900

CPC分类号： A61K47/183 ,  A61K9/0019 ,  A61K47/10 ,  A61K47/12 ,  A61K47/14

摘要： Novel combination of preservatives (methyl and propyl parabens, benzyl alcohol, and 2-phenoxyethanol) were found to pass antimicrobial testing according to USP, BP, and EP. The new preservatives were put into vaccines using L-histidine as a buffer to keep pH at 7.0. HPLC methods were developed to analyze these preservatives and their degradation products.

摘要翻译： 发现防腐剂（甲基和丙基对羟基苯甲酸酯，苯甲醇和2-苯氧基乙醇）的新型组合根据USP，BP和EP通过抗菌试验。 使用L-组氨酸作为缓冲液将新的防腐剂投入疫苗中以将pH保持在7.0。 开发了HPLC方法来分析这些防腐剂及其降解产物。

公开(公告)号：US06773705B1

公开(公告)日：2004-08-10

申请号：US09258682

申请日：1999-02-26

申请人： Denise L. Faustman ,  Takuma Hayashi

发明人： Denise L. Faustman ,  Takuma Hayashi

IPC分类号： A61K3900

CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4703 ,  C12N9/1205 ,  C12N9/6421 ,  C12Q1/37 ,  G01N33/564 ,  G01N2333/9121 ,  G01N2333/96425 ,  G01N2500/04 ,  G01N2800/042

摘要： The invention provides a method of detecting autoimmune disease in a mammal, comprising providing a biological sample from a mammal and detecting proteasome activity, wherein a reduction in proteasome activity from a basal state is indicative of autoimmune disease. In addition, the invention encompasses a method of treating an autoimmune disease in a mammal, comprising administering to a mammal suspected of suffering from an autoimmune disease an agent which restores NF&kgr;B activity in an amount and for a time sufficient to result in normal NF&kgr;B activity in the mammal.

摘要翻译： 本发明提供一种检测哺乳动物自身免疫性疾病的方法，其包括从哺乳动物提供生物样品和检测蛋白酶体活性，其中来自基础状态的蛋白酶体活性的降低指示自身免疫性疾病。 此外，本发明包括治疗哺乳动物自身免疫性疾病的方法，包括对怀疑患有自身免疫性疾病的哺乳动物施用足以导致NFκB活性正常的量的NFκB活性的药物 哺乳动物。

公开(公告)号：US06761890B1

公开(公告)日：2004-07-13

申请号：US09659983

申请日：2000-09-12

申请人： Robert Hans Meloen ,  Hendrica Berendina Oonk

发明人： Robert Hans Meloen ,  Hendrica Berendina Oonk

IPC分类号： A61K3900

CPC分类号： C07K7/23 ,  A61K39/00 ,  C07K14/723

摘要： A peptide that comprises a modified tandem GnRH decapeptide sequence which allows for a testosterone level that is essentially non-detectable after vaccination with the peptide in a suitable dosage and/or allows for a an immunogenic response that allows for the effective discrimination between GnRH-I and GnRH-II and a method for the immunocastration of pigs.

摘要翻译： 一种肽，其包含修饰的串联GnRH十肽序列，其允许在以适合剂量接种肽后接种疫苗时基本上不可检测的睾酮水平和/或允许免疫原性反应，其允许有效区分GnRH-I 和GnRH-II和猪免疫接种的方法。

公开(公告)号：US06746674B2

公开(公告)日：2004-06-08

申请号：US09971187

申请日：2001-10-05

申请人： Jian Ni ,  Guo-Liang Yu ,  Reiner L. Gentz

发明人： Jian Ni ,  Guo-Liang Yu ,  Reiner L. Gentz

IPC分类号： A61K3900

CPC分类号： G01N33/57415 ,  A61K38/00 ,  C07K14/475

摘要： The invention relates to cytostatin II growth modulatory polypeptides, polynucleotides encoding the polypeptides, methods for producing the polypeptides, in particular by expressing the polynucleotides, and agonists and antagonists of the polypeptides. The invention further relates to methods for utilizing such polynucleotides, polypeptides, agonists and antagonists for applications, which relate, in part, to research, diagnostic and clinical arts.

摘要翻译： 本发明涉及细胞抑素II生长调节性多肽，编码多肽的多核苷酸，特别是通过表达多核苷酸产生多肽的方法，多肽的激动剂和拮抗剂。 本发明还涉及用于应用的这些多核苷酸，多肽，激动剂和拮抗剂的方法，其部分涉及研究，诊断和临床领域。

公开(公告)号：US06743428B1

公开(公告)日：2004-06-01

申请号：US10088548

申请日：2002-03-15

申请人： Jihoon Chang ,  Jang Seong Kim ,  Eun Jeong Park ,  Jung-sun Yum ,  Soo-Il Chung

发明人： Jihoon Chang ,  Jang Seong Kim ,  Eun Jeong Park ,  Jung-sun Yum ,  Soo-Il Chung

IPC分类号： A61K3900

CPC分类号： C07K14/4703 ,  A61K38/00

摘要： The present invention provides a novel angiogenesis inhibitor, LK68 whose amino acid sequence is identical with the human apolipoprotein (a) kringle domains IV36, IV37 and V38, a cDNA sequence encoding the LK68, a recombinant expression vector comprising the cDNA, a recombinant microorganism transformed with the recombinant expression vector and a novel use of the LK68 as an anticancer agent and a method for treating angiogenesis-mediated disease. LK68, LK6, LK7 and LK8 exhibit inhibitory activities on the cultured endothelial cell proliferation as well as on the endothelial cell migration. LK68 and its single kringles also inhibit the normal development of capillaries in the chick embryo chorioallantoic membrane (CAM). It was also showed that systemic administration of LK68 causes the inhibition of primary tumor growth, which is correlated with a suppression of tumor-induced angiogenesis. Accordingly, LK68 protein, its single kringles or their functional equivalents may be applied for the development of a potent anti-cancer agent, which is highly effective for angiogenesis-mediated diseases covering cancer, rheumatoid arthritis, psoriasis, ocular angiogenic disease, etc.

摘要翻译： 本发明提供了一种新型的血管生成抑制剂LK68，其氨基酸序列与人载脂蛋白（a）三环结构域IV36，IV37和V38相同，编码LK68的cDNA序列，包含cDNA的重组表达载体，转化的重组微生物 用重组表达载体和LK68作为抗癌剂的新用途和治疗血管生成介导的疾病的方法。 LK68，LK6，LK7和LK8表现出对培养的内皮细胞增殖以及内皮细胞迁移的抑制活性。 LK68及其单克隆也抑制鸡胚绒毛尿囊膜（CAM）毛细血管的正常发育。 还表明，LK68的全身给药导致原发性肿瘤生长的抑制，其与抑制肿瘤诱导的血管发生相关。 因此，LK68蛋白，其单克隆或其功能等同物可用于开发有效的抗癌剂，其对于涵盖癌症，类风湿性关节炎，牛皮癣，眼血管生成疾病等的血管生成介导的疾病是非常有效的。

公开(公告)号：US06723321B2

公开(公告)日：2004-04-20

申请号：US09227488

申请日：1999-01-06

申请人： E. Peter Greenberg ,  John E. Cronan, Jr. ,  Bryce V. Plapp ,  Matthew R. Parsek

发明人： E. Peter Greenberg ,  John E. Cronan, Jr. ,  Bryce V. Plapp ,  Matthew R. Parsek

IPC分类号： A61K3900

CPC分类号： C07H19/16 ,  A61K31/7076 ,  C07H19/207 ,  C12Q1/25 ,  Y02A50/473 ,  Y10S424/832

摘要： Provided are compositions and methods useful for modulating the activity of autoinducer synthase catalysts. A method for identifing modulators of the autoinducer synthesis reaction is also provided. Such modulators are useful for controlling bacterial growth and can be used for therapeutic treatment of bacterial infections particularly in immunocompromised subjects. They are also useful in treating disease states associated with autoinducer synthesis and biofilm development.

摘要翻译： 提供可用于调节自动诱导合成酶催化剂活性的组合物和方法。 还提供了用于鉴定自动诱导剂合成反应的调节剂的方法。 这样的调节剂可用于控制细菌生长，并且可用于细菌感染的治疗性治疗，特别是在免疫受损的受试者中。 它们也可用于治疗与自身诱导剂合成和生物膜发育相关的疾病状态。

公开(公告)号：US06716432B1

公开(公告)日：2004-04-06

申请号：US08378213

申请日：1995-01-25

申请人： James Cleland Paton ,  David John Hansman ,  Graham John Boulnois ,  Peter William Andrew ,  Timothy John Mitchell ,  John Arthur Walker

发明人： James Cleland Paton ,  David John Hansman ,  Graham John Boulnois ,  Peter William Andrew ,  Timothy John Mitchell ,  John Arthur Walker

IPC分类号： A61K3900

CPC分类号： C07K14/3156 ,  A61K39/00

摘要： Mutants of pneumolysin that are non-toxic by reason of amino acid substitutions have been constructed. These mutants elicit an immune response in animals that is reactive to wild-type pneumolysin. The invention also encompasses vaccines for humans based on these mutants, including vaccines comprising conjugates with pneumococcal capsular polysaccharides.

摘要翻译： 已经构建了因氨基酸取代而无毒的肺炎球菌突变体。 这些突变体在与野生型肺炎溶菌素反应的动物中引起免疫应答。 本发明还包括基于这些突变体的人的疫苗，包括包含与肺炎球菌荚膜多糖的缀合物的疫苗。