公开(公告)号：US20240069022A1

公开(公告)日：2024-02-29

申请号：US18272540

申请日：2022-01-14

申请人： ASAHI KASEI KABUSHIKI KAISHA

发明人： Katsuyoshi TAKAHASHI ,  Kotaro ODA ,  Mika SUGATA

IPC分类号： G01N33/569 ,  C07K16/12

CPC分类号： G01N33/56916 ,  C07K16/1228 ,  G01N2333/24 ,  G01N2469/10

摘要： Provided is a method that enables the presence and/or amount of bacteria of Enterobacteriaceae in a food/drink sample, an environmental sample, or a biological sample to be detected easily and efficiently in a short amount of time. Said method includes a step for simultaneously detecting, on the basis of an antigen-antibody reaction, the presence and/or amount of bacteria of Enterobacteriaceae of at least two different genera in a sample.

公开(公告)号：US09320788B2

公开(公告)日：2016-04-26

申请号：US13923211

申请日：2013-06-20

申请人： Qu Biologics Inc.

发明人： Harold David Gunn

IPC分类号： A61K39/108 ,  A61K39/00 ,  A61K45/06 ,  G01N33/50

CPC分类号： A61K39/0258 ,  A61K39/0011 ,  A61K45/06 ,  A61K2039/521 ,  A61K2039/5252 ,  G01N33/5011 ,  G01N33/5047 ,  G01N33/5082 ,  G01N2333/24 ,  G01N2333/31 ,  A61K2300/00

摘要： The invention provides in part methods of treating cancers of a specific organ or tissue by administering a composition that is antigenically specific for one or more microbes that are pathogenic in the specific organ or tissue in which the cancer is situated. The formulations of the invention thereby facilitate activation of an immune response to a cancer in a particular tissue or organ. The compositions may for example include killed or attenuated microbial pathogens, such as whole killed bacterial cells, and may be administered at sites distant from the cancer, for example the skin. In some embodiments, microbial species of endogenous flora or exogenous microbial pathogens that are known to cause infection in the relevant organ or tissue may be used in the formulation of the antigenic compositions.

摘要翻译： 本发明部分地通过给予在癌症所在的特定器官或组织中具有致病性的一种或多种微生物具有抗原性特异性的组合物来治疗特定器官或组织的癌症的方法。 因此，本发明的制剂有助于激活对特定组织或器官中的癌症的免疫应答。 组合物可以例如包括灭活或减毒的微生物病原体，例如完全杀死的细菌细胞，并且可以在远离癌症的位置（例如皮肤）施用。 在一些实施方案中，已知在相关器官或组织中引起感染的内源性菌群或外来微生物病原体的微生物物种可用于抗原组合物的制剂中。

公开(公告)号：US20140272938A1

公开(公告)日：2014-09-18

申请号：US14203436

申请日：2014-03-10

申请人： Theranos, Inc.

发明人： Alexander Loo ,  Elizabeth A. Holmes

IPC分类号： C12Q1/04 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C12Q1/04 ,  B01F11/0266 ,  B01F11/0283 ,  B01F2215/0037 ,  B01L2300/0609 ,  B01L2400/0439 ,  C12N1/066 ,  G01N1/286 ,  G01N1/38 ,  G01N35/00722 ,  G01N2001/386 ,  G01N2035/00554 ,  G01N2035/00891 ,  G01N2333/24 ,  G01N2333/31

摘要： Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

摘要翻译： 提供了包括用于样品制备的超声波发生器的装置，系统和方法。 超声波仪可用于混合，重悬，雾化，分散，分解或除气。 超声波仪器可用于破坏细胞，如样品中的病原体细胞。 样品制备可以包括通过超声处理暴露病原体识别材料以检测，鉴定或测量病原体。 超声波发生器可通过将其尖端接触到含有样品的容器的外壁将超声能量转移到样品溶液。 包括超声波发生器的多用途装置还包括用于附加动作和测定的其它组件。 设备和包括这种设备的系统可以与用于样品测定和分析的系统中的实验室或其他设备通信。 提供了利用这种装置和系统的方法。 改进的样品制备装置，系统和方法可用于分析样品，例如 用于诊断病原体感染的患者。

公开(公告)号：US08501198B2

公开(公告)日：2013-08-06

申请号：US12843296

申请日：2010-07-26

申请人： Harold David Gunn

发明人： Harold David Gunn

IPC分类号： A61K39/108

CPC分类号： A61K39/0258 ,  A61K39/0011 ,  A61K45/06 ,  A61K2039/521 ,  A61K2039/5252 ,  G01N33/5011 ,  G01N33/5047 ,  G01N33/5082 ,  G01N2333/24 ,  G01N2333/31 ,  A61K2300/00

摘要： The invention provides in part methods of treating cancers of a specific organ or tissue by administering a composition that is antigenically specific for one or more microbes that are pathogenic in the specific organ or tissue in which the cancer is situated. The formulations of the invention thereby facilitate activation of an immune response to a cancer in a particular tissue or organ. The compositions may for example include killed or attenuated microbial pathogens, such as whole killed bacterial cells, and may be administered at sites distant from the cancer, for example the skin. In some embodiments, microbial species of endogenous flora that are known to cause infection in the relevant organ or tissue may be used in the formulation of the antigenic compositions. In alternative embodiments, exogenous microbial pathogens that are known to cause infection in the relevant organ or tissue may be used in the formulation of the antigenic compositions. The administration of the immunogenic compositions may be repeated relatively frequently over a relatively long period of time. In embodiments for intradermal or subcutaneous injection, dosages may be adjusted so that injections reproduce a consistent, visible, delayed inflammatory immune reaction at the successive site or sites of administration.

摘要翻译： 本发明部分地通过给予在癌症所在的特定器官或组织中具有致病性的一种或多种微生物具有抗原性特异性的组合物来治疗特定器官或组织的癌症的方法。 因此，本发明的制剂有助于激活对特定组织或器官中的癌症的免疫应答。 组合物可以例如包括灭活或减毒的微生物病原体，例如完全杀死的细菌细胞，并且可以在远离癌症的位置（例如皮肤）施用。 在一些实施方案中，已知在相关器官或组织中引起感染的内源性菌群的微生物物种可用于抗原组合物的制剂中。 在替代实施方案中，已知在相关器官或组织中引起感染的外源微生物病原体可用于抗原组合物的制剂中。 免疫原性组合物的施用可以在相当长的时间内相对频繁地重复。 在皮内注射或皮下注射的实施方案中，可以调整剂量，使得注射在连续的施用位点或再生部位再现一致的，可见的，延迟的炎性免疫反应。

公开(公告)号：US20080090268A1

公开(公告)日：2008-04-17

申请号：US11892163

申请日：2007-08-20

申请人： Sitke Aygen

发明人： Sitke Aygen

IPC分类号： C12Q1/02

CPC分类号： C12Q1/04 ,  G01N33/62 ,  G01N2333/205 ,  G01N2333/24

摘要： The method for the diagnosis of Helicobacter pylori infection by the oral administration of defined amounts of 13C-labeled urea and examination for 13C content of blood samples removed at a defined time is effected by a) removing from 0.1 to 0.6 ml of capillary blood from the finger or ear lobe of a patient or venous blood of a patient, in both cases with an empty stomach before the beginning of the test; b) administering an exact amount of from 10 to 50 mg of 13C-urea in aqueous solution with a pH value of 2 to 4 to the patient; c) again removing capillary or venous blood exactly after 10 to 15 min from the administration; and d) determining the 13C content of the blood samples by isotope ratio mass spectrometry (IRMS), and deducing the presence of Helicobacter pylori from the increase of the 13C values.

摘要翻译： 用于诊断幽门螺杆菌感染的方法通过口服施用限定量的13 C-标记的尿素并检查在规定时间内除去的血液样品的13 C含量 通过以下方式实现：a）在试验开始之前，在空腹的两种情况下，从患者的手指或耳叶或患者的静脉血中除去0.1至0.6ml毛细血管; b）向患者施用精确量的10至50mg的13-C尿素水溶液，pH值为2至4; c）在给药10至15分钟后，再次清除毛细血管或静脉血; 和d）通过同位素比质谱法（IRMS）测定血液样本的“13”C含量，并从增加的13 C值中推断幽门螺杆菌的存在 。

公开(公告)号：US20070004021A1

公开(公告)日：2007-01-04

申请号：US11168830

申请日：2005-06-28

申请人： Lawrence Restaino

发明人： Lawrence Restaino

IPC分类号： C12N1/20

CPC分类号： C12N1/20 ,  C12Q1/045 ,  C12Q1/10 ,  G01N2333/24

摘要： A plating media for identifying Yersinia pestis bacteria having nutrients for promoting growth of Yersinia pestis thereby producing beta-glucosidase, carbohydrate that is incapable of reacting with Yersinia pestis, a pH indicator dye, a chromogenic substrate that reacts to beta-glucosidase to form precipitate, and an agent to solidify the mixture, whereby a microorganism which ferments the carbohydrate but does not produce beta-glucosidase will produce colonies of the color determined by the pH indicator dye, Yersinia pestis and other microorganisms that do not ferment the carbohydrate but produce beta-glucosidase activate the substrate to color their colonies with the color of precipitate released by the substrate, and other bacteria which ferment the carbohydrate and produce beta-glucosidase produce colonies of the color that results from mixing the colors described above.

摘要翻译： 一种用于鉴定具有促进鼠疫耶尔森疫霉生长的营养物质的耶尔森氏菌属细菌的电镀培养基，从而产生β-葡糖苷酶，不能与鼠疫耶尔森菌（Yersinia pestis）反应的碳水化合物，pH指示剂染料，与β-葡糖苷酶反应形成沉淀物的显色底物， 和固化混合物的试剂，由此发酵碳水化合物但不产生β-葡糖苷酶的微生物将产生由pH指示剂染料，鼠疫耶尔森氏菌和其他不发酵碳水化合物但产生β-葡糖苷酶的微生物所确定的颜色的菌落， 葡萄糖苷酶激活底物以使底物着色的沉淀物着色，并且发酵碳水化合物并产生β-葡糖苷酶的其它细菌产生由上述颜色混合产生的颜色的菌落。

公开(公告)号：US4952497A

公开(公告)日：1990-08-28

申请号：US140501

申请日：1988-01-04

申请人： Saumya Bhaduri ,  Lucille K. Conway ,  Reynato V. Lachica

发明人： Saumya Bhaduri ,  Lucille K. Conway ,  Reynato V. Lachica

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N2333/24 ,  Y10S435/822

摘要： A crystal-violet dye binding technique useful for the detection and differentiation of virulent plasmid-bearing strains of Yersinia enterocolitica. Virulent plasmid-bearing strains of the bacteria bind the crystal violet dye to form dark violet colonies while avirulent plasmidless strains fail to bind the dye and remain white in color. The method is simple, rapid, economical and highly reliable.

摘要翻译： 一种结晶紫色染料结合技术，可用于检测和分化具有毒力的小肠结肠炎耶尔森氏菌菌株。 细菌的有毒的携带质粒的菌株结合紫色染料形成深紫色菌落，而无毒无质粒菌株不能结合染料并保持白色。 该方法简单，快速，经济，可靠性高。

公开(公告)号：US20180238883A1

公开(公告)日：2018-08-23

申请号：US15957679

申请日：2018-04-19

申请人： TransEnterix Surgical, Inc.

发明人： Matthew R. Penny ,  Kevin Andrew Hufford ,  Nicholas J. Bender ,  Alexander John Maret ,  Paul Wilhelm Schnur ,  Sevan Abashian ,  Dustin Vaughan

IPC分类号： G01N33/573 ,  G01N33/68

CPC分类号： G01N33/573 ,  A61B34/30 ,  A61B34/37 ,  A61B34/76 ,  A61B46/00 ,  A61B46/10 ,  A61B2017/00477 ,  A61B2034/302 ,  G01N33/6893 ,  G01N2333/24 ,  G01N2333/25 ,  G01N2333/922 ,  G01N2800/065 ,  G01N2800/52

摘要： A robotic surgical system includes a fluid drive system and a surgical instrument removably positioned in operative engagement with the drive system. A sterile barrier covers non-sterile portions of the surgical system. Features of the sterile barrier are used to transfer motion output from the fluid drive system to the instrument for actuation of the instrument.

公开(公告)号：US20170157099A1

公开(公告)日：2017-06-08

申请号：US15039116

申请日：2014-11-24

申请人： The Regents of the University of California Santa Cruz

发明人： Victoria Auerbach Stone ,  Roger G. Linington ,  Weng Ruh Wong ,  Miles C. Duncan

IPC分类号： A61K31/44 ,  G01N33/94 ,  G01N33/50

CPC分类号： A61K31/44 ,  A61K39/02 ,  C12Q1/18 ,  G01N33/5008 ,  G01N33/94 ,  G01N2333/24 ,  G01N2333/986 ,  G01N2560/00

摘要： Described herein are assays for identifying piericidins and piericidin compositions.

公开(公告)号：US20160146808A1

公开(公告)日：2016-05-26

申请号：US14943623

申请日：2015-11-17

申请人： NESTEC SA

发明人： Richard Nelson ,  Ebenzer Satyaraj

IPC分类号： G01N33/566

CPC分类号： G01N33/566 ,  A61B5/411 ,  A61B5/6889 ,  A61G10/02 ,  G01N33/68 ,  G01N2333/24 ,  G01N2333/46

摘要： The present disclosure provides an apparatus comprising an air circulating device, an allergen source comprising an allergen, and a housing. The housing can be collapsible, portable, disposable and configurable. The present disclosure also provides a method comprising positioning an allergen source comprising an allergen in a chamber, distributing at least a portion of the allergen from the allergen source within the chamber, and collecting at least a portion of the distributed allergen. In an embodiment, the level of the shed allergen can be used to measure the effectiveness of various measures implemented to reduce airborne Fel d1 that is emitted from soiled cat litter. In another embodiment, the allergen can be introduced at a desired level in the chamber, and the resultant symptoms of a subject (e.g. a human) within the chamber can be identified and correlated with the amount of allergen.

摘要翻译： 本公开提供了一种包括空气循环装置，包含变应原的变应原源和壳体的装置。 外壳可折叠，便携，一次性和可配置。 本公开还提供了一种方法，其包括将包含过敏原的变应原源定位在室中，将来自变应原源的变应原的至少一部分分配在室内，以及收集分布式过敏原的至少一部分。 在一个实施方案中，棚棚过敏原的水平可用于测量为减少从污染的猫砂发出的空气中的Fel d1所实施的各种措施的有效性。 在另一个实施方案中，可以在室中以期望的水平引入变应原，并且可以鉴定室内受试者（例如人）的最终症状并与变应原的量相关。