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    Novel methods for therapeutic vaccination
    3.
    发明申请
    Novel methods for therapeutic vaccination 有权
    治疗性接种的新​​方法

    公开(公告)号：US20060008465A1

    公开(公告)日：2006-01-12

    申请号：US11202516

    申请日：2005-08-11

    申请人： Lucilla Steinaa ,  Soren Mouritsen ,  Anand Gautam ,  Iben Dalum ,  Jesper Hanning ,  Dana Leach ,  Klaus Nielsen ,  Gunilla Karlsson ,  Peter Rasmussen

    发明人： Lucilla Steinaa ,  Soren Mouritsen ,  Anand Gautam ,  Iben Dalum ,  Jesper Hanning ,  Dana Leach ,  Klaus Nielsen ,  Gunilla Karlsson ,  Peter Rasmussen

    IPC分类号： C07H21/04 ,  A61K39/00 ,  C07K14/82 ,  C12N1/21 ,  C12N1/18

    CPC分类号： C07K16/3069 ,  A61K39/0011 ,  C07K14/50 ,  C07K14/705 ,  C07K14/70539 ,  C07K14/71 ,  C07K2317/34 ,  C12N2799/021 ,  Y02A50/412

    摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.

    摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。

    Purification of her-2 variants
    4.
    发明申请
    Purification of her-2 variants 有权
    纯化她-2变体

    公开(公告)号：US20060240511A1

    公开(公告)日：2006-10-26

    申请号：US10560961

    申请日：2004-06-24

    申请人： Marie Eskling ,  Klaus Nielsen

    发明人： Marie Eskling ,  Klaus Nielsen

    IPC分类号： C07K14/71 ,  C12P21/06

    CPC分类号： C07K14/71

    摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.

    摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），c）尺寸排阻层析（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。

    Method of improving indoor air quality by thermally inactivating fungi on building surfaces
    7.
    发明授权
    Method of improving indoor air quality by thermally inactivating fungi on building surfaces 有权
    通过热灭活建筑物表面真菌来提高室内空气质量的方法

    公开(公告)号：US06187263B1

    公开(公告)日：2001-02-13

    申请号：US09117784

    申请日：1998-08-07

    申请人： Klaus Nielsen

    发明人： Klaus Nielsen

    IPC分类号： A61L208

    CPC分类号： A01M21/04 ,  A01M19/00

    摘要： A method of improving the indoor air quality in a building comprising at least partially inactivating fungal mycelia and spores associated with a building material surface by applying onto the surface thermal energy initially contained in water vapour at a pressure exceeding 1 bar, in an amount which is sufficient to at least partially inactivate said fungal mycelia and spores but essentially without causing damages to the surface material.

    摘要翻译： 一种改善建筑物中的室内空气质量的方法，其包括至少部分地灭活与建筑材料表面相关联的真菌菌丝体和孢子，所述真菌菌丝体和构件材料表面的压力超过1巴，最初包含在水蒸气中的热能，其量为 足以使所述真菌菌丝体和孢子至少部分失活，但基本上不会对表面材料造成损害。