公开(公告)号：US20060240511A1

公开(公告)日：2006-10-26

申请号：US10560961

申请日：2004-06-24

申请人： Marie Eskling ,  Klaus Nielsen

发明人： Marie Eskling ,  Klaus Nielsen

IPC分类号： C07K14/71 ,  C12P21/06

CPC分类号： C07K14/71

摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.

摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），c）尺寸排阻层析（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。

公开(公告)号：US08022039B2

公开(公告)日：2011-09-20

申请号：US12824420

申请日：2010-06-28

申请人： Marie Eskling ,  Klaus Gregorius Nielsen

发明人： Marie Eskling ,  Klaus Gregorius Nielsen

IPC分类号： A61K38/16 ,  A61K38/17 ,  C07K14/00 ,  C07K14/435 ,  C07K14/705

CPC分类号： C07K14/71

摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), C) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.

摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），C）尺寸排阻色谱法（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。

公开(公告)号：US07763452B2

公开(公告)日：2010-07-27

申请号：US10560961

申请日：2004-06-24

申请人： Marie Eskling ,  Klaus Gregorius Nielsen

发明人： Marie Eskling ,  Klaus Gregorius Nielsen

IPC分类号： C12N15/00 ,  C12N15/11 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10

CPC分类号： C07K14/71

摘要： The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein for which the purification process has been especially adapted, as well as means for the preparation of the variant.

摘要翻译： 本发明提供了从昆虫细胞培养物获得的EGFR家族蛋白的纯化的新方法。 该方法包括以下步骤：a）将缓冲液进行渗滤和培养基交换，b）固定化金属亲和层析（IMAC），c）尺寸排阻层析（SEC）和d）阴离子交换层析（AIE）。 该方法还提供了HER-2蛋白的免疫原性变体，其纯化方法已被特别适用，以及制备该变体的方法。