公开(公告)号：US20150120486A1

公开(公告)日：2015-04-30

申请号：US14171327

申请日：2014-02-03

申请人： Mark Haidet ,  Josef Utz ,  John Heyman ,  Karen Leytze ,  Bentley Heyman ,  Pete Reilly

发明人： Mark Haidet ,  Josef Utz ,  John Heyman ,  Karen Leytze ,  Bentley Heyman ,  Pete Reilly

IPC分类号： G06Q30/06

CPC分类号： G06Q30/0611

摘要： A computer-implemented method and system that allows operators to compile and optionally compare prices across multiple suppliers. The system and method may be based on operator preferences and may be designed to execute orders from a single database and operator interface. The system and method also facilitate the ability to compare pricing and track historical variations and trends across like products and across a plurality of suppliers by a data mapping structure.

摘要翻译： 一种计算机实现的方法和系统，允许操作员编译并可选地比较多个供应商的价格。 系统和方法可以基于操作者偏好，并且可以被设计为从单个数据库和操作者界面执行订单。 该系统和方法还有助于通过数据映射结构来比较定价和跟踪同类产品和跨多个供应商的历史变化和趋势的能力。

公开(公告)号：US20110275063A1

公开(公告)日：2011-11-10

申请号：US13003417

申请日：2009-07-10

申请人： David A. Weitz ,  John Heyman

发明人： David A. Weitz ,  John Heyman

IPC分类号： C12Q1/68 ,  C12Q1/02 ,  G01N33/53 ,  C12P21/00

CPC分类号： G01N33/5008 ,  G01N33/5436 ,  G01N33/6854

摘要： The present invention generally relates to fluidic droplets, and techniques for screening or sorting such fluidic droplets. In some embodiments, the fluidic droplets may contain cells (e.g., hybridoma cells) that can secrete various species, such as antibodies, for example. In one aspect, a plurality of fluidic droplets containing cells is screened to determine proteins, antibodies, polypeptides, peptides, nucleic acids, or the like. For example, cells able to secrete species such as antibodies may be se according to certain embodiments of the invention. Examples of such cells include, for instance, immortal cells such as hybridomas, or non-immortal cells such as B-cells. For instance, blood cells may be encapsulated within a plurality of fluidic droplets, and the cells able to produce antibodies may be determined. In some cases, expression or secretion levels may be determined using signaling entities, for example, determinable microparticles present within the fluidic droplet. Other aspects of the invention relate to kits involving such fluidic droplets, methods of promoting the making or use of such fluidic droplets, and the like.

摘要翻译： 本发明一般涉及流体液滴，以及用于筛选或分类这种流体液滴的技术。 在一些实施方案中，流体液滴可以含有可分泌各种物质例如抗体的细胞（例如，杂交瘤细胞）。 在一个方面，筛选含有细胞的多个流体液滴以确定蛋白质，抗体，多肽，肽，核酸等。 例如，根据本发明的某些实施方案，能够分泌诸如抗体的物种的细胞可能是se。 这些细胞的实例包括，例如，诸如杂交瘤的永生细胞，或非永生细胞如B细胞。 例如，血细胞可以被包封在多个流体液滴中，并且可以确定能产生抗体的细胞。 在一些情况下，表达或分泌水平可以使用信号实体来确定，例如存在于流体液滴内的可测定的微粒。 本发明的其它方面涉及涉及这种流体液滴的试剂盒，促进制造或使用这种流体液滴的方法等。

公开(公告)号：US1559871A

公开(公告)日：1925-11-03

申请号：US70276024

申请日：1924-03-29

申请人： JOHN HEYMAN

发明人： JOHN HEYMAN

IPC分类号： D06H7/02 ,  E21B17/043

CPC分类号： E21B17/043 ,  D06H7/02 ,  Y10T83/4541 ,  Y10T83/4592

公开(公告)号：US20050282184A1

公开(公告)日：2005-12-22

申请号：US11053187

申请日：2005-02-07

申请人： Jonathan Chesnut ,  Stewart Shuman ,  Knut Madden ,  John Heyman ,  Robert Bennett

发明人： Jonathan Chesnut ,  Stewart Shuman ,  Knut Madden ,  John Heyman ,  Robert Bennett

IPC分类号： A01K67/027 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C40B40/02 ,  C40B50/06 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/66 ,  C12N15/10 ,  C12N15/64

摘要： The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

公开(公告)号：US20120015382A1

公开(公告)日：2012-01-19

申请号：US13195468

申请日：2011-08-01

申请人： David A. Weitz ,  Andrew Griffiths ,  Sarah Koester ,  Vamsi K. Mootha ,  Honey Duan ,  Jeremy Agresti ,  Christoph Merten ,  John Heyman ,  John R. Gilbert

发明人： David A. Weitz ,  Andrew Griffiths ,  Sarah Koester ,  Vamsi K. Mootha ,  Honey Duan ,  Jeremy Agresti ,  Christoph Merten ,  John Heyman ,  John R. Gilbert

IPC分类号： G01N33/53 ,  G01N21/64

CPC分类号： G01N33/505 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502784 ,  G01N15/1459 ,  G01N33/5052 ,  G01N33/5436 ,  G01N33/6854 ,  G01N2015/149

摘要： The present invention generally relates to fluidic droplets, and techniques for screening or sorting such fluidic droplets. In some embodiments, the fluidic droplets may contain cells (e.g., hybridoma cells) that can secrete various species, such as antibodies, for example. In one aspect, a plurality of fluidic droplets containing cells is screened to determine proteins, antibodies, polypeptides, peptides, nucleic acids, or the like. For example, cells able to secrete species such as antibodies may be selected according to certain embodiments of the invention. Examples of such cells include, for instance, immortal cells such as hybridomas, or non-immortal cells such as B-cells. For instance, blood cells may be encapsulated within a plurality of fluidic droplets, and the cells able to produce antibodies may be determined. In some cases, expression or secretion levels may be determined using signaling entities, for example, determinable microparticles present within the fluidic droplet. Other aspects of the invention relate to kits involving such fluidic droplets, methods of promoting the making or use of such fluidic droplets, and the like.

摘要翻译： 本发明一般涉及流体液滴，以及用于筛选或分类这种流体液滴的技术。 在一些实施方案中，流体液滴可以含有可分泌各种物质例如抗体的细胞（例如，杂交瘤细胞）。 在一个方面，筛选含有细胞的多个流体液滴以确定蛋白质，抗体，多肽，肽，核酸等。 例如，可以根据本发明的某些实施方案来选择能够分泌诸如抗体的物质的细胞。 这些细胞的实例包括，例如，诸如杂交瘤的永生细胞，或非永生细胞如B细胞。 例如，血细胞可以被包封在多个流体液滴中，并且可以确定能产生抗体的细胞。 在一些情况下，表达或分泌水平可以使用信号实体来确定，例如存在于流体液滴内的可测定的微粒。 本发明的其它方面涉及涉及这种流体液滴的试剂盒，促进制造或使用这种流体液滴的方法等。

公开(公告)号：US20090068170A1

公开(公告)日：2009-03-12

申请号：US12172186

申请日：2008-07-11

申请人： David A. Weitz ,  Andrew Griffiths ,  Sarah Koester ,  Vamsi K. Mootha ,  Honey Duan ,  Jeremy Agresti ,  Christoph Merten ,  John Heyman ,  John R. Gilbert

发明人： David A. Weitz ,  Andrew Griffiths ,  Sarah Koester ,  Vamsi K. Mootha ,  Honey Duan ,  Jeremy Agresti ,  Christoph Merten ,  John Heyman ,  John R. Gilbert

IPC分类号： A61K39/395 ,  C12Q1/02 ,  G01N33/53 ,  A01N1/02

CPC分类号： G01N33/505 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502784 ,  G01N15/1459 ,  G01N33/5052 ,  G01N33/5436 ,  G01N33/6854 ,  G01N2015/149

摘要： The present invention generally relates to fluidic droplets, and techniques for screening or sorting such fluidic droplets. In some embodiments, the fluidic droplets may contain cells (e.g., hybridoma cells) that can secrete various species, such as antibodies, for example. In one aspect, a plurality of fluidic droplets containing cells is screened to determine proteins, antibodies, polypeptides, peptides, nucleic acids, or the like. For example, cells able to secrete species such as antibodies may be selected according to certain embodiments of the invention. Examples of such cells include, for instance, immortal cells such as hybridomas, or non-immortal cells such as B-cells. For instance, blood cells may be encapsulated within a plurality of fluidic droplets, and the cells able to produce antibodies may be determined. In some cases, expression or secretion levels may be determined using signaling entities, for example, determinable microparticles present within the fluidic droplet. Other aspects of the invention relate to kits involving such fluidic droplets, methods of promoting the making or use of such fluidic droplets, and the like.

摘要翻译： 本发明一般涉及流体液滴，以及用于筛选或分类这种流体液滴的技术。 在一些实施方案中，流体液滴可以含有可分泌各种物质例如抗体的细胞（例如，杂交瘤细胞）。 在一个方面，筛选含有细胞的多个流体液滴以确定蛋白质，抗体，多肽，肽，核酸等。 例如，可以根据本发明的某些实施方案来选择能够分泌诸如抗体的物质的细胞。 这些细胞的实例包括，例如，诸如杂交瘤的永生细胞，或非永生细胞如B细胞。 例如，血细胞可以被包封在多个流体液滴中，并且可以确定能产生抗体的细胞。 在一些情况下，表达或分泌水平可以使用信号实体来确定，例如存在于流体液滴内的可测定的微粒。 本发明的其它方面涉及涉及这种流体液滴的试剂盒，促进制造或使用这种流体液滴的方法等。