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1.发明申请PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS USING GENES ENCODING POLYPEPTIDES HAVING THIOLASE, HMG-COA SYNTHASE AND HMG-COA REDUCTASE ENZYMATIC ACTIVITIES 有权使用编码具有THIOLASE，HMG-COA合成酶和HMG-COA还原酶活性的多肽的基因生产甲酸，异丙肾上腺素和异维A公开(公告)号：US20120276603A1
公开(公告)日：2012-11-01
申请号：US13459033
申请日：2012-04-27
申请人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
发明人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/1025 , C12N9/1029 , C12N15/70 , C12N15/81 , C12P5/002 , C12P7/02 , C12P7/04 , C12P7/42 , C12Y101/01034 , C12Y203/01016 , C12Y203/0301 , Y02E50/343
摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
摘要翻译： 本发明的特征在于通过来自生物李斯特氏菌DSM 20601，屎肠球菌，，肠球菌EG2的mvaE和mvaS基因的异源表达增加微生物中甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯生产的组合物和方法， 和肠球菌（Enterococcus casseliflavus）。
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2.发明申请RECOMBINANT MICROORGANISMS FOR ENHANCED PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS 有权用于增强生产甲酸，异丙肾上腺素和异丙肾上腺素的重组微生物公开(公告)号：US20120276587A1
公开(公告)日：2012-11-01
申请号：US13459067
申请日：2012-04-27
申请人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
发明人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
IPC分类号： C12P5/02 , C12N1/15 , C12N1/19 , C12P7/42 , C12P23/00 , C12P7/00 , C12P17/18 , C12P7/02 , C12P5/00 , C12N1/21 , C12P7/04
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/18 , C12N9/88 , C12N15/70 , C12P5/002 , C12P5/026 , C12P7/02 , C12P7/04 , C12P7/42 , C12Y101/01027 , C12Y101/01034 , C12Y101/01037 , C12Y102/01051 , C12Y102/04001 , C12Y203/01008 , C12Y203/01016 , C12Y203/03001 , C12Y203/0301 , C12Y207/02001 , C12Y301/01031 , C12Y401/01031 , Y02E50/343
摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
摘要翻译： 本发明的特征在于通过在以下酶促途径中通过工程化微生物来增加碳通量以产生甲羟戊酸来生产甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的组合物和方法：（a）柠檬酸合酶 ）磷酸转乙酰酶，（c）乙酸激酶，（d）乳酸脱氢酶，（e）苹果酸酶和（f）丙酮酸脱氢酶，使得其中一种酶活性被调节。 此外，通过mvaE和mvaS基因的异源表达（例如但不限于来自生物体李斯特氏菌DSM的mvaE和mvaS基因）可以进一步增强甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的生产 20601，屎肠球菌，，肠球菌EG2，和黑麦草肠球菌）。
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3.发明授权Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities 有权使用编码具有硫解酶，HMG-CoA合成酶和HMG-CoA还原酶酶活性的多肽的基因生产甲羟戊酸，异戊二烯和类异戊二烯公开(公告)号：US08889383B2
公开(公告)日：2014-11-18
申请号：US13459033
申请日：2012-04-27
申请人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
发明人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/1025 , C12N9/1029 , C12N15/70 , C12N15/81 , C12P5/002 , C12P7/02 , C12P7/04 , C12P7/42 , C12Y101/01034 , C12Y203/01016 , C12Y203/0301 , Y02E50/343
摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus.
摘要翻译： 本发明的特征在于通过来自生物李斯特氏菌DSM 20601，屎肠球菌，，肠球菌EG2的mvaE和mvaS基因的异源表达增加微生物中甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯生产的组合物和方法， 和肠球菌（Enterococcus casseliflavus）。
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公开(公告)号：US20130260432A1
公开(公告)日：2013-10-03
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
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公开(公告)号：US20130045891A1
公开(公告)日：2013-02-21
申请号：US13283564
申请日：2011-10-27
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
摘要翻译： 本发明提供包含至少一种具有提高的比生产率的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于增加宿主细胞中异戊二烯生产的变体植物异戊二烯合成酶。
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6.发明授权Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids 有权用于增强甲羟戊酸，异戊二烯和类异戊二烯生产的重组微生物公开(公告)号：US09121038B2
公开(公告)日：2015-09-01
申请号：US13459067
申请日：2012-04-27
申请人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
发明人： Zachary Q. Beck , Michael C. Miller , Caroline M. Peres , Yuliya A. Primak , Jeff P. Pucci , Derek H. Wells
IPC分类号： C12N1/20 , C12N1/00 , C12P5/02 , C07H21/04 , C12P5/00 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/18 , C12N9/88 , C12P7/02 , C12P7/04 , C12P7/42
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/18 , C12N9/88 , C12N15/70 , C12P5/002 , C12P5/026 , C12P7/02 , C12P7/04 , C12P7/42 , C12Y101/01027 , C12Y101/01034 , C12Y101/01037 , C12Y102/01051 , C12Y102/04001 , C12Y203/01008 , C12Y203/01016 , C12Y203/03001 , C12Y203/0301 , C12Y207/02001 , C12Y301/01031 , C12Y401/01031 , Y02E50/343
摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
摘要翻译： 本发明的特征在于通过在以下酶促途径中通过工程化微生物来增加碳通量以产生甲羟戊酸来生产甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的组合物和方法：（a）柠檬酸合酶 ）磷酸转乙酰酶，（c）乙酸激酶，（d）乳酸脱氢酶，（e）苹果酸酶和（f）丙酮酸脱氢酶，使得其中一种酶活性被调节。 此外，通过mvaE和mvaS基因的异源表达（例如但不限于来自生物体李斯特氏菌DSM的mvaE和mvaS基因）可以进一步增强甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的生产 20601，屎肠球菌，，肠球菌EG2，和黑麦草肠球菌）。
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7.发明授权公开(公告)号：US08916370B2
公开(公告)日：2014-12-23
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12P21/06 , C12N15/00 , C12P5/02 , C12N1/20 , C12N1/00 , C07H21/04 , C07K1/00 , C12P5/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。 特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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8.发明授权公开(公告)号：US08518686B2
公开(公告)日：2013-08-27
申请号：US12765825
申请日：2010-04-22
申请人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
发明人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
CPC分类号： C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
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9.发明申请THREE-DIMENSIONAL STRUCTURE OF ISOPRENE SYNTHASE AND ITS USE THEREOF FOR GENERATING VARIANTS 有权异丙肾上腺素的三维结构及其用于产生变异体公开(公告)号：US20110076743A1
公开(公告)日：2011-03-31
申请号：US12765825
申请日：2010-04-22
申请人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
发明人： Zachary Quinn Beck , Richard R. Bott , Christopher Lee Rife , Derek H. Wells , Jeffrey V. Miller
CPC分类号： C12P5/007 , C07K2299/00 , C12N9/88 , C12Y402/03027
摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译： 本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。 本发明还提供使用三维结构设计异戊二烯合酶的方法，所述异戊二烯合酶具有改进的微生物宿主细胞中异戊二烯生产增加的活性。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。
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公开(公告)号：US07390646B2
公开(公告)日：2008-06-24
申请号：US10944256
申请日：2004-09-17
CPC分类号： A61K39/13 , A61K39/00 , A61K39/12 , A61K48/00 , A61K2039/522 , A61K2039/523 , A61K2039/53 , A61K2039/542 , C12N2770/24134 , C12N2770/24143 , C12N2770/32634 , C12N2770/32643 , Y02A50/388 , Y02A50/403 , Y02A50/466 , Y02A50/476
摘要： The present invention provides a live, attenuated, invasive bacterium that infects a mammalian host cell, and releases exogenous RNA into the cytoplasm of the host cell. The present invention further provides compositions, including immunogenic compositions, comprising a subject bacterium. The present invention provides methods of delivering an RNA to a eukaryotic host cell in vitro, ex vivo, or in vivo. The present invention provides methods of delivering a protein to a host cell in vitro, ex vivo, or in vivo. The present invention provides methods of controlling expression of a target gene in a eukaryotic host cell. The present invention provides methods of inducing an immune response in a mammalian host to a polypeptide antigen, the method involving administering to the host a subject bacterium, wherein the antigen is encoded by the exogenous RNA produced by the bacterium.
摘要翻译： 本发明提供感染哺乳动物宿主细胞的活的，减毒的侵入性细菌，并将外源性RNA释放到宿主细胞的细胞质中。 本发明还提供了包含受试细菌的组合物，包括免疫原性组合物。 本发明提供了在体外，离体或体内将RNA递送至真核宿主细胞的方法。 本发明提供了在体外，离体或体内将蛋白递送至宿主细胞的方法。 本发明提供了控制靶基因在真核宿主细胞中的表达的方法。 本发明提供了在哺乳动物宿主中诱导多肽抗原的免疫应答的方法，所述方法涉及向所述宿主施用受试细菌，其中所述抗原由所述细菌产生的外源RNA编码。
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