公开(公告)号：US20210301313A1

公开(公告)日：2021-09-30

申请号：US16499072

申请日：2018-03-28

Applicant: Boehringer Ingelheim RCV GmbH & Co KG ,  SANDOZ AG ,  Validogen GmbH ,  BIOMIN HOLDING GMBH ,  Lonza Ltd

Inventor： Karlheinz Grillitsch ,  Guenther Daum ,  Andreas Grutsch

IPC: C12P21/02 ,  C12N15/67 ,  C12N15/81 ,  C07K16/00

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

公开(公告)号：US09677107B2

公开(公告)日：2017-06-13

申请号：US14135389

申请日：2013-12-19

Applicant: Sandoz AG ,  Boehringer Ingelheim RCV GmbH & Co KG

Inventor： Maria Reitmeir ,  Rainer Schneider ,  Bernhard Auer

IPC: C12P21/06 ,  C12N15/70 ,  C12P21/02 ,  C12N9/50

CPC classification number: C12P21/06 ,  C07K2319/50 ,  C12N9/506 ,  C12N15/70 ,  C12P21/02

Abstract: Disclosed is a method for producing a recombinant protein of interest, the method being characterized in by the following steps: (a) providing a fusion protein comprising an Npro autoprotease moiety and a protein of interest moiety in inclusion bodies, (b) solubilizing the inclusion bodies, (c) allowing the fusion protein to be cleaved by the Npro autoprotease moiety under chaotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein and wherein the recombinant protein of interest is not yet renatured or simultaneously renatured, and (d) recovering the protein of interest, optionally including a renaturing step for the protein of interest.

公开(公告)号：US12123044B2

公开(公告)日：2024-10-22

申请号：US17942774

申请日：2022-09-12

Applicant: Boehringer Ingelheim RCV GmbH & Co KG ,  LONZA LTD. ,  VALIDOGEN GBMH

Inventor： Karlheinz Grillitsch ,  Guenther Daum ,  Andreas Grutsch

IPC: C12N15/80 ,  C07K16/00 ,  C12N15/67 ,  C12N15/81 ,  C12P21/02

CPC classification number: C12P21/02 ,  C07K16/00 ,  C12N15/67 ,  C12N15/815 ,  C07K2317/55

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

公开(公告)号：US10661264B2

公开(公告)日：2020-05-26

申请号：US16103473

申请日：2018-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: B01J39/20 ,  B01J39/04 ,  C12N9/02 ,  C07K1/18 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  B01D15/36 ,  C07K14/435 ,  C07K16/00

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US10590427B2

公开(公告)日：2020-03-17

申请号：US15304504

申请日：2015-04-16

Applicant: Boehringer Ingelheim RCV GmbH & Co KG ,  Validogen GmbH ,  Lonza Ltd.

Inventor： Brigitte Gasser ,  Diethard Mattanovich ,  Markus Buchetics

IPC: C12N15/81 ,  C12P21/00

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

公开(公告)号：US20190046969A1

公开(公告)日：2019-02-14

申请号：US16103473

申请日：2018-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: B01J39/20 ,  B01J39/04 ,  B01J41/05 ,  B01J39/07 ,  B01J39/05 ,  B01J41/07 ,  C07K16/00 ,  C07K14/435 ,  B01D15/36 ,  B01J47/04 ,  B01J41/20 ,  B01J39/26 ,  C07K1/18 ,  B01J41/14 ,  C12N9/02

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US20240310805A1

公开(公告)日：2024-09-19

申请号：US18442010

申请日：2024-02-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ GMBH

Inventor： Alois Jungbauer ,  Astrid Durauer ,  Nicole Walch ,  Dominik Sauer ,  Theresa Scharl-Hirsch ,  Michael Melcher ,  Friedrich Leisch

IPC: G05B19/042 ,  G01N21/64 ,  G01N30/88 ,  G05B19/418

CPC classification number: G05B19/0428 ,  G01N21/6486 ,  G01N30/88 ,  G05B19/4183 ,  G05B19/41885

Abstract: The invention relates to a method and device which allows in real-time the determination of concentration, purity and potency of a biological product during purification and/or concentration processes in order to intervene into the process, either for process control or real time release. The properties of the process stream are continuously monitored by at least two online sensors and with the aid of multivariate statistical analysis so that concentration, purity and potency is determined in real time.

公开(公告)号：US10076749B2

公开(公告)日：2018-09-18

申请号：US14913337

申请日：2014-08-25

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: C07K14/00 ,  B01J39/20 ,  B01J39/04 ,  C12N9/02 ,  C07K1/18 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  B01D15/36 ,  C07K14/435 ,  C07K16/00 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07

CPC classification number: B01J39/20 ,  B01D15/362 ,  B01D15/363 ,  B01J39/04 ,  B01J39/05 ,  B01J39/07 ,  B01J39/26 ,  B01J41/05 ,  B01J41/07 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  C07K1/18 ,  C07K14/43595 ,  C07K16/00 ,  C12N9/0089 ,  C12Y115/01001

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US20220380739A1

公开(公告)日：2022-12-01

申请号：US17635215

申请日：2020-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG

Inventor： Alois JUNGBAUER ,  Nico LINGG ,  Gerald STRIEDNER ,  Monika CSERJAN-PUSCHMANN ,  Chris OOSTENBRINK ,  Christoph OEHLKNECHT ,  Christina KROESS ,  Petra ENGELE ,  Rainer SCHNEIDER

IPC: C12N9/14 ,  C12N15/70

Abstract: The invention refers to a single-chain circular permuted caspase-2 comprising the following structure from N- to C-terminus: i) a small subunit of a caspase-2, or a functionally active variant thereof; and ii) a large subunit of a caspase-2, or a functionally active variant thereof, wherein said cp caspase-2 comprises one or more amino acid substitutions increasing P1′ tolerance of said cp caspase-2 compared to a cp caspase-2 without said amino acid substitutions.

公开(公告)号：US20210269811A1

公开(公告)日：2021-09-02

申请号：US17254238

申请日：2019-06-27

Applicant: Boehringer Ingelheim RCV GmbH & CO KG ,  Validogen GmbH ,  Lonza Ltd

Inventor： Richard Zahrl ,  Jonas Burgard ,  Kristin Baumann ,  Diethard Mattanovich ,  Brigitte Gasser

IPC: C12N15/81 ,  C07K16/00

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield of a protein of interest (POI) in a eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4/2. The invention relates further to a recombinant eukaryotic host cell for manufacturing a POI, wherein the host cell is engineered to overexpress at least one polynucleotide encoding at least one transcription factor as well as the use of the host cell for manufacturing a POI.