公开(公告)号：US09677107B2

公开(公告)日：2017-06-13

申请号：US14135389

申请日：2013-12-19

Applicant: Sandoz AG ,  Boehringer Ingelheim RCV GmbH & Co KG

Inventor： Maria Reitmeir ,  Rainer Schneider ,  Bernhard Auer

IPC: C12P21/06 ,  C12N15/70 ,  C12P21/02 ,  C12N9/50

CPC classification number: C12P21/06 ,  C07K2319/50 ,  C12N9/506 ,  C12N15/70 ,  C12P21/02

Abstract: Disclosed is a method for producing a recombinant protein of interest, the method being characterized in by the following steps: (a) providing a fusion protein comprising an Npro autoprotease moiety and a protein of interest moiety in inclusion bodies, (b) solubilizing the inclusion bodies, (c) allowing the fusion protein to be cleaved by the Npro autoprotease moiety under chaotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein and wherein the recombinant protein of interest is not yet renatured or simultaneously renatured, and (d) recovering the protein of interest, optionally including a renaturing step for the protein of interest.