公开(公告)号：US10661264B2

公开(公告)日：2020-05-26

申请号：US16103473

申请日：2018-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: B01J39/20 ,  B01J39/04 ,  C12N9/02 ,  C07K1/18 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07 ,  B01D15/36 ,  C07K14/435 ,  C07K16/00

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US20190046969A1

公开(公告)日：2019-02-14

申请号：US16103473

申请日：2018-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: B01J39/20 ,  B01J39/04 ,  B01J41/05 ,  B01J39/07 ,  B01J39/05 ,  B01J41/07 ,  C07K16/00 ,  C07K14/435 ,  B01D15/36 ,  B01J47/04 ,  B01J41/20 ,  B01J39/26 ,  C07K1/18 ,  B01J41/14 ,  C12N9/02

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US10537887B2

公开(公告)日：2020-01-21

申请号：US14913333

申请日：2014-08-25

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov ,  Moritz Imendoerffer

IPC: B01J39/20 ,  B01J39/04 ,  C12N9/02 ,  C07K1/18 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  B01D15/36 ,  C07K14/435 ,  C07K16/00 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07

Abstract: The present invention provides a novel adsorbent composition for recovering biomolecules from a fluid. The composition comprises positively and negatively charged microparticles in the form of ground particles. The adsorbent is particularly useful for purification of biomolecules from the cell culture.

公开(公告)号：US10076749B2

公开(公告)日：2018-09-18

申请号：US14913337

申请日：2014-08-25

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer Hahn ,  Alois Jungbauer ,  Alexandru Trefilov

IPC: C07K14/00 ,  B01J39/20 ,  B01J39/04 ,  C12N9/02 ,  C07K1/18 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  B01D15/36 ,  C07K14/435 ,  C07K16/00 ,  B01J39/05 ,  B01J39/07 ,  B01J41/05 ,  B01J41/07

CPC classification number: B01J39/20 ,  B01D15/362 ,  B01D15/363 ,  B01J39/04 ,  B01J39/05 ,  B01J39/07 ,  B01J39/26 ,  B01J41/05 ,  B01J41/07 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  C07K1/18 ,  C07K14/43595 ,  C07K16/00 ,  C12N9/0089 ,  C12Y115/01001

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

公开(公告)号：US20210149361A1

公开(公告)日：2021-05-20

申请号：US16091095

申请日：2017-04-04

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Alois Jungbauer ,  Astrid Durauer ,  Nicole Walch ,  Dominik Sauer ,  Theresa Scharl-Hirsch ,  Michael Melcher ,  Friedrich Leisch

IPC: G05B19/042 ,  G05B19/418

Abstract: The invention relates to a method and device which allows in real-time the determination of concentration, purity and potency of a biological product during purification and/or concentration processes in order to intervene into the process, either for process control or real time release. The properties of the process stream are continuously monitored by at least two online sensors and with the aid of multivariate statistical analysis so that concentration, purity and potency is determined in real time.

公开(公告)号：US20240310805A1

公开(公告)日：2024-09-19

申请号：US18442010

申请日：2024-02-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ GMBH

Inventor： Alois Jungbauer ,  Astrid Durauer ,  Nicole Walch ,  Dominik Sauer ,  Theresa Scharl-Hirsch ,  Michael Melcher ,  Friedrich Leisch

IPC: G05B19/042 ,  G01N21/64 ,  G01N30/88 ,  G05B19/418

CPC classification number: G05B19/0428 ,  G01N21/6486 ,  G01N30/88 ,  G05B19/4183 ,  G05B19/41885

Abstract: The invention relates to a method and device which allows in real-time the determination of concentration, purity and potency of a biological product during purification and/or concentration processes in order to intervene into the process, either for process control or real time release. The properties of the process stream are continuously monitored by at least two online sensors and with the aid of multivariate statistical analysis so that concentration, purity and potency is determined in real time.