公开(公告)号：US20220380739A1

公开(公告)日：2022-12-01

申请号：US17635215

申请日：2020-08-14

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG

Inventor： Alois JUNGBAUER ,  Nico LINGG ,  Gerald STRIEDNER ,  Monika CSERJAN-PUSCHMANN ,  Chris OOSTENBRINK ,  Christoph OEHLKNECHT ,  Christina KROESS ,  Petra ENGELE ,  Rainer SCHNEIDER

IPC: C12N9/14 ,  C12N15/70

Abstract: The invention refers to a single-chain circular permuted caspase-2 comprising the following structure from N- to C-terminus: i) a small subunit of a caspase-2, or a functionally active variant thereof; and ii) a large subunit of a caspase-2, or a functionally active variant thereof, wherein said cp caspase-2 comprises one or more amino acid substitutions increasing P1′ tolerance of said cp caspase-2 compared to a cp caspase-2 without said amino acid substitutions.