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180 条记录 (耗时 0.053 秒)

    Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis
    2.
    发明授权
    Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis 有权

    公开(公告)号：US10908164B2

    公开(公告)日：2021-02-02

    申请号：US15460491

    申请日：2017-03-16

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Peter Slade

    IPC分类号： G01N33/68 ,  G01N27/447 ,  C12Q1/34

    摘要： An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.

    Nucleic acid amplification
    5.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10329544B2

    公开(公告)日：2019-06-25

    申请号：US15091717

    申请日：2016-04-06

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao ,  Wolfgang Hinz

    IPC分类号： C12Q1/68 ,  C12N9/12 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

    摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Polymerase compositions, methods of making and using same
    6.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US10240134B2

    公开(公告)日：2019-03-26

    申请号：US15481858

    申请日：2017-04-07

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC分类号： C12N9/12 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869

    摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    Nucleic acid amplification
    7.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US10113195B2

    公开(公告)日：2018-10-30

    申请号：US14789922

    申请日：2015-07-01

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC分类号： C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6874

    摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Methods for detecting defects in inorganic-coated polymer surfaces
    8.
    发明授权
    Methods for detecting defects in inorganic-coated polymer surfaces 有权
    检测无机涂层聚合物表面缺陷的方法

    公开(公告)号：US09310315B2

    公开(公告)日：2016-04-12

    申请号：US14192711

    申请日：2014-02-27

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Yu-Zhong Zhang

    IPC分类号： G01N31/22 ,  G01N21/91 ,  C07F5/02

    CPC分类号： G01N21/91 ,  C07F5/022 ,  Y10T428/24355

    摘要： Lipophilic fluorescent substances can be used to detect surface defects in materials having hydrophilic (e.g., inorganic) coatings. Use of the described methods makes surface defects appear fluorescent, while the remaining surfaces are not labeled. The disclosed methods are inexpensive, rapid, and easy alternatives to existing approaches.

    摘要翻译： 亲脂性荧光物质可用于检测具有亲水（例如无机）涂层的材料中的表面缺陷。 使用所述方法会使表面缺陷出现荧光，而其余表面未标记。 所公开的方法是便宜，快速和容易的替代现有方法。

    Cyanine compounds and their application as quenching compounds
    10.
    发明授权
    Cyanine compounds and their application as quenching compounds 有权
    花青化合物及其作为淬灭化合物的应用

    公开(公告)号：US09150922B2

    公开(公告)日：2015-10-06

    申请号：US14109475

    申请日：2013-12-17

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    IPC分类号： C07D401/04 ,  C07D209/14 ,  C12Q1/68 ,  C07D209/12 ,  C07D209/24 ,  C09B23/12

    CPC分类号： C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

    摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

    摘要翻译： 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。