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    Polymerase compositions and kits, and methods of using and making the same
    1.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US11866740B2

    公开(公告)日：2024-01-09

    申请号：US17302621

    申请日：2021-05-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter B. Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q1/686 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Polymerase compositions, methods of making and using same
    3.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09976128B2

    公开(公告)日：2018-05-22

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    Polymerase compositions, methods of making and using same
    4.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09657281B2

    公开(公告)日：2017-05-23

    申请号：US14502201

    申请日：2014-09-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Daniel Mazur ,  Theo Nikiforov ,  Mindy Landes ,  Eileen Tozer

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME
    7.
    发明申请
    POLYMERASE COMPOSITIONS AND METHODS OF MAKING AND USING SAME 有权
    聚合物组合物及其制备方法和使用方法

    公开(公告)号：US20160177373A1

    公开(公告)日：2016-06-23

    申请号：US14970818

    申请日：2015-12-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12Q1/68 ,  C12N9/12

    CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q1/6869 ,  C12Q2521/101 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a reference Taq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

    Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了修饰的聚合酶和其生物活性片段，例如修饰的Taq聚合酶，其允许改善的核酸扩增。 在一些方面，本公开提供与参考Taq聚合酶相比具有改进的热稳定性，准确性，持续性和/或读取长度的修饰聚合酶。 在一些方面，本公开涉及可用于扩增方法的修饰聚合酶或其生物活性片段，并且在实际示例性实施方案中涉及乳液PCR。

    Polymerase compositions and methods
    8.
    发明授权
    Polymerase compositions and methods 有权
    聚合酶组合物和方法

    公开(公告)号：US09365839B2

    公开(公告)日：2016-06-14

    申请号：US14710160

    申请日：2015-05-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

    IPC: C12N9/12 ,  C07H19/20 ,  C12Q1/68

    CPC classification number: C12Q1/6869 ,  C07H19/20 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6818 ,  C12Y207/07 ,  C12Y207/07007 ,  G01N21/6428 ,  G01N33/582 ,  G01N2021/6432

    Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

    Abstract translation: 本文公开了显示改变的聚合酶活性的修饰聚合酶组合物，其可用于多种生物应用。 本文还公开了制备和使用这些组合物的方法。 在一些实施方案中，组合物显示改变的性质，其可以增强其在各种生物应用中的效用。 这种改变的性质可以包括例如改变的核苷酸结合亲和力，改变的核苷酸掺入动力学，改变的光稳定性和/或改变的纳米颗粒耐受性，以及本文公开的一系列其它性质。

    Polymerase compositions and methods of making and using same
    9.
    发明授权
    Polymerase compositions and methods of making and using same 有权

    公开(公告)号：US12215383B2

    公开(公告)日：2025-02-04

    申请号：US17725369

    申请日：2022-04-20

    Applicant: Life Technologies Corporation

    Inventor： Daniel Mazur ,  Eileen Tozer ,  Sihong Chen ,  Peter Vander Horn ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/686 ,  C12Q1/6869

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

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