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    Substituted Anthraquinone Dyes for Cellular Stains and Enzyme Detection
    1.
    发明申请
    Substituted Anthraquinone Dyes for Cellular Stains and Enzyme Detection 有权
    取代的蒽醌染料用于细胞污染和酶检测

    公开(公告)号：US20150025222A1

    公开(公告)日：2015-01-22

    申请号：US14248886

    申请日：2014-04-09

    Applicant: Biotium, Inc.

    Inventor： Fei MAO ,  Wai-Yee LEUNG ,  Ching-Ying CHEUNG ,  Lori Michelle ROBERTS

    IPC: C09B1/516 ,  C09B1/51 ,  A61K49/00

    CPC classification number: C09B1/516 ,  A61K49/0052 ,  C09B1/501 ,  C09B1/51 ,  C09B1/5155 ,  G01N1/30 ,  G01N21/6428

    Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.

    Abstract translation: 本发明公开了可用作细胞污渍的新的取代的蒽醌染料。 在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。 本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

    Substituted anthraquinone dyes for cellular stains and enzyme detection
    2.
    发明授权
    Substituted anthraquinone dyes for cellular stains and enzyme detection 有权
    取代的蒽醌染料用于细胞染色和酶检测

    公开(公告)号：US08790892B2

    公开(公告)日：2014-07-29

    申请号：US13439821

    申请日：2012-04-04

    Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

    Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Lori Michelle Roberts

    IPC: G01N1/30

    CPC classification number: C09B1/516 ,  A61K49/0052 ,  C09B1/501 ,  C09B1/51 ,  C09B1/5155 ,  G01N1/30 ,  G01N21/6428

    Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.

    Abstract translation: 本发明公开了可用作细胞污渍的新的取代的蒽醌染料。 在本发明的某些方面，核染料可用于染色死细胞或固定细胞的细胞核。 本发明的另一方面涉及包含酶底物部分的取代的蒽醌染料，其可由酶转化或切割，使得底物部分的转化或切割导致染料的官能度或光谱性质的可检测变化。

    Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology
    3.
    发明申请
    Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology 有权
    使用染料与核酸染色或检测及相关技术相结合的方法

    公开(公告)号：US20140073058A1

    公开(公告)日：2014-03-13

    申请号：US13962668

    申请日：2013-08-08

    Applicant: Biotium, Inc.

    Inventor： Fei Mao ,  Wai-Yee Leung

    IPC: G01N21/64

    CPC classification number: C09B15/00 ,  C09B11/28 ,  C09B23/04 ,  C12Q1/6818 ,  G01N1/30 ,  G01N21/6486 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/301

    Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

    Abstract translation: 提供了使用染料和相关技术的方法。 可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。 这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。 可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。 在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光，可以用于染色和/或检测核酸。

    Dyes and labeled molecules
    4.
    发明授权
    Dyes and labeled molecules 有权
    染料和标记分子

    公开(公告)号：US08530195B2

    公开(公告)日：2013-09-10

    申请号：US12820983

    申请日：2010-06-22

    Applicant: Fei Mao ,  Wai-Yee Leung ,  Xing Xin

    Inventor： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

    IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/04

    CPC classification number: C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

    Abstract: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

    Abstract translation: 提供二聚和三聚体核酸染料以及相关系统和方法。 这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。 在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。 本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

    Methods of using dyes in association with nucleic acid staining or detection and associated technology
    5.
    发明授权
    Methods of using dyes in association with nucleic acid staining or detection and associated technology 有权
    使用染料与核酸染色或检测及相关技术相结合的方法

    公开(公告)号：US08232050B2

    公开(公告)日：2012-07-31

    申请号：US12854436

    申请日：2010-08-11

    Applicant: Fei Mao ,  Wai-Yee Leung

    Inventor： Fei Mao ,  Wai-Yee Leung

    IPC: C12Q1/68 ,  C07H21/00 ,  C07H19/04

    CPC classification number: C09B15/00 ,  C09B11/28 ,  C09B23/04 ,  C12Q1/6818 ,  G01N1/30 ,  G01N21/6486 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/301

    Abstract: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with a strong acid and a cation that is associated with a strong base may be used in such an application. A dimeric dye, such as a dimeric dye capable of forming a hairpin-like structure, may be used to stain and/or detect nucleic acids via a release-on-demand mechanism. A dimeric dye having low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, may be used to stain and/or detect nucleic acids.

    Abstract translation: 提供了使用染料和相关技术的方法。 可以在核酸凝胶染色应用和/或核酸检测应用中使用染料，例如单体染料或二聚染料。 这种染料和包含与强酸相关的阴离子和与强碱相关的阳离子的盐可用于这种应用中。 可以使用二聚染料，例如能够形成发夹状结构的二聚染料，以通过按需释放机制对核酸进行染色和/或检测。 在存在核酸时具有低背景荧光的二聚染料和在存在核酸时与核酸存在时的高荧光可用于染色和/或检测核酸。

    ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS
    9.
    发明申请
    ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS 有权

    公开(公告)号：US20160237421A9

    公开(公告)日：2016-08-18

    申请号：US14051187

    申请日：2013-10-10

    Applicant: Biotium, Inc.

    Inventor： Fei Mao ,  Hui Cen ,  Wai-Yee Leung

    IPC: C12N9/96

    CPC classification number: C12N9/96 ,  C07D263/60 ,  C07D417/06 ,  C07D501/00 ,  C07K7/06 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/485 ,  G01N21/6486 ,  G01N33/582

    Abstract: Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

    Luminescent metal complexes and associated technology
    10.
    发明授权
    Luminescent metal complexes and associated technology 有权
    发光金属配合物及相关技术

    公开(公告)号：US09193746B2

    公开(公告)日：2015-11-24

    申请号：US11952867

    申请日：2007-12-07

    Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

    Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Jie Yang

    IPC: G01N21/64 ,  C07F15/00 ,  C09B57/10 ,  G01N33/68

    CPC classification number: C07F15/0053 ,  C09B57/10 ,  G01N33/6839 ,  G01N2458/40

    Abstract: Luminescent metal complexes, methods of producing and/or designing same, methods of using same, and associated technology, are disclosed herein. A luminescent metal complex may be useful for a variety of applications, such as staining, detection, and/or identification, for example, of substances, such as poly(amino acids), for example. Further by way of example, a luminescent metal complex may be useful for staining, detecting, and/or identifying poly(amino acids) that are associated with any of various environments, such as a gel or a gel matrix, such as any associated with SDS-PAGE, for example, a surface environment, such as any associated with western blot, for example, and/or the like. Compositions, solutions, and kits comprising a luminescent metal complex are also disclosed herein.

    Abstract translation: 本文公开了发光金属络合物，其制备和/或设计方法，使用方法及相关技术。 发光金属络合物可用于各种应用，例如染色，检测和/或鉴定，例如物质，例如聚（氨基酸）。 进一步作为示例，发光金属络合物可用于染色，检测和/或鉴定与各种环境中的任何环境相关的聚（氨基酸），例如凝胶或凝胶基质，例如任何与 例如，SDS-PAGE，例如表面环境，例如与western印迹相关的任何例如和/或类似物。 本文还公开了包含发光金属络合物的组合物，溶液和试剂盒。

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