公开(公告)号：US20170299539A1

公开(公告)日：2017-10-19

申请号：US15485871

申请日：2017-04-12

Applicant: Polymer Technology Systems, Inc.

Inventor： Gary L. Hughes

IPC: G01N27/327 ,  G01N33/92 ,  C12Q1/00 ,  G01N33/543

CPC classification number: G01N27/3272 ,  C12Q1/005 ,  G01N33/5438 ,  G01N33/92 ,  G01N2333/902 ,  G01N2333/90203 ,  G01N2333/9121 ,  G01N2333/91215 ,  G01N2333/92

Abstract: A system for the electrochemical detection of triglyceride levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

公开(公告)号：US09766243B2

公开(公告)日：2017-09-19

申请号：US14413743

申请日：2013-07-09

Applicant: SCHEBO BIOTECH AG

Inventor： Hans Scheefers ,  Ursula Scheefers-Borchel

IPC: G01N33/574 ,  G01N33/558 ,  G01N33/573 ,  G01N33/72

CPC classification number: G01N33/57419 ,  G01N33/558 ,  G01N33/573 ,  G01N33/57446 ,  G01N33/726 ,  G01N2333/91215

Abstract: The invention relates to a test kit for better carrying out a method for detecting biomarkers in human or animal stool, which can serve as an indication of a pathological, particularly a malignant event in the gastrointestinal tract (esophagus, stomach, small bowel, biliary tract, pancreas, and bowel).The invention teaches a novel and more efficient methods, uses and embodiments of a combined rapid test. The combined rapid test cassette used for implementing the test kit and the optimally coordinated reagents thereof contains two lateral flow test strips for the synchronous—in the technical meaning—detection of the biomarkers M2-PK and the biomarker hemoglobin.The test serves as a “dual filter” for diagnosing probands as part of a colon cancer screening program. The test is very cost-efficient and cuts costs in the health system by the examination at an early stage of colon cancer and the consequences thereof.

公开(公告)号：US20170184565A1

公开(公告)日：2017-06-29

申请号：US15116319

申请日：2015-02-12

Applicant: Thomas M. ROBERTS ,  Haoxuan TONG ,  Jean J. ZHAO ,  John BLENIS ,  Dana-Farber Cancer Institute, Inc. ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Thomas M. Roberts ,  Haoxuan Tong ,  Jean Zhao ,  John Blenis

IPC: G01N33/50 ,  A61K45/06 ,  A61K31/58 ,  G01N33/574 ,  C12Q1/68

CPC classification number: G01N33/5011 ,  A61K31/58 ,  A61K45/06 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/5088 ,  G01N33/57492 ,  G01N2333/91215 ,  G01N2500/10

Abstract: The present invention is based, in part, on the identification of novel FI.3K-mTORCI-S6K 1 signaling pathway biomarkers predictive of responsiveness to anti-cancer therapies.

公开(公告)号：US20170122872A1

公开(公告)日：2017-05-04

申请号：US15299085

申请日：2016-10-20

Applicant: General Electric Company

Inventor： Anup Sood ,  ARUNKUMAR NATARAJAN ,  Dmitry Dylov ,  Lakshmi Sireesha Kaanumalle ,  Elizabeth Mary McDonough

IPC: G01N21/64 ,  G01N33/574 ,  G01N33/483

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N33/4833 ,  G01N33/57407 ,  G01N2021/6439 ,  G01N2333/70503 ,  G01N2333/91215

Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.

公开(公告)号：US20170114387A1

公开(公告)日：2017-04-27

申请号：US15301133

申请日：2015-03-17

Applicant: SHIGA UNIVERSITY OF MEDICAL SCIENCE

Inventor： Shin-ya MORITA

IPC: C12Q1/28 ,  G01N21/64 ,  C12Q1/32 ,  C12Q1/44 ,  C12Q1/48

CPC classification number: C12Q1/28 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  C12Q1/485 ,  C12Y101/03021 ,  C12Y111/01007 ,  C12Y207/0103 ,  C12Y301/04004 ,  G01N21/6428 ,  G01N33/92 ,  G01N2333/902 ,  G01N2333/908 ,  G01N2333/91215 ,  G01N2333/916 ,  G01N2405/04

Abstract: The inventions disclosed herein are a method for quantifying cardiolipin in a sample, comprising the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprising phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.

公开(公告)号：US20170067900A1

公开(公告)日：2017-03-09

申请号：US15357762

申请日：2016-11-21

Applicant: AROCELL AB

Inventor： Sven SKOG ,  Staffan ERIKSSON ,  Bernard TRIBUKAIT ,  Qimin HE

IPC: G01N33/574

CPC classification number: G01N33/57488 ,  G01N2333/91215 ,  G01N2800/54

Abstract: Method of determining a likelihood of cancer relapse in a subject who has completed cancer tumor surgery, radiotherapy treatment and/or chemotherapy treatment comprises contacting an antibody that binds specifically to a serum form of thymidine kinase 1 (STK1) protein with a blood serum sample one to six months after completing the surgery and/or treatment, and before any cancer relapse has been detected; determining an amount of antibody binding to STK1 protein in the sample; correlating the amount of antibody binding to STK1 protein to a concentration of STK1 protein in the sample; and based on the concentration of STK1 protein in the sample, generating decision support information representative of a likelihood of cancer relapse in the subject one to ten years after completion of the surgery and/or treatment, the decision support information comprising a likelihood value defining one of a high or low likelihood of cancer relapse.

Abstract translation: 确定完成癌肿瘤手术，放射治疗和/或化学疗法治疗的受试者中癌症复发的可能性的方法包括将与血清形式的胸苷激酶1（STK1）蛋白特异性结合的抗体与血清样品一 在完成手术和/或治疗后6个月，并且在检测到任何癌症复发之前; 测定样品中与STK1蛋白结合的抗体量; 将抗体与STK1蛋白的结合量与样品中STK1蛋白的浓度相关联; 并且基于样品中STK1蛋白的浓度，产生代表手术和/或治疗完成后1至10年在受试者中癌症复发的可能性的决策支持信息，所述决策支持信息包括定义一个的似然值 癌症复发的可能性很高或很低。

公开(公告)号：US20160313301A1

公开(公告)日：2016-10-27

申请号：US15103830

申请日：2014-12-03

Applicant: NIPPON KAYAKU KABUSHIKI KAISHA

Inventor： Tsuyoshi FUKUDA ,  Osamu MIYAZAKI ,  Kuniko MASUDA ,  Kazuya OKAMOTO

IPC: G01N33/50

CPC classification number: G01N33/5011 ,  C12Q1/485 ,  G01N33/574 ,  G01N2333/91205 ,  G01N2333/91215

Abstract: A method for determining sensitivity of a tumor to a HSP90 inhibitor, including: measuring a phosphorylation level of Akt or a phosphorylation level of ERK in a tissue of the tumor from a patient.

Abstract translation: 用于确定肿瘤对HSP90抑制剂的敏感性的方法，包括：测量来自患者的肿瘤组织中Akt的磷酸化水平或ERK的磷酸化水平。

公开(公告)号：US20160289728A1

公开(公告)日：2016-10-06

申请号：US15037814

申请日：2014-11-21

Applicant: The University of Tokyo

Inventor： Kazuo KUMAGAI ,  Takayoshi OKABE ,  Hirotatsu KOJIMA ,  Tetsuo NAGANO

IPC: C12Q1/00 ,  C12Q1/48 ,  C12Q1/42 ,  C12Q1/32

CPC classification number: C12Q1/008 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/485 ,  G01N2333/90209 ,  G01N2333/91091 ,  G01N2333/91097 ,  G01N2333/91215

Abstract: In a method for detecting fluorescence or absorbance of the present invention, a diaphorase causes reduction from resazurin to resorufin in the presence of an SH reagent and NADH or NADPH, and the resulting fluorescence intensity or absorbance is measured. A method for measuring ADP of the present invention includes a 2-1 process in which glucose is reacted with ADP and an ADP-dependent hexokinase, a 2-2 process in which the glucose-6-phosphate obtained in the 2-1 process is reacted with NAD or NADP and glucose-6-phosphate dehydrogenase, and a 2-3 process in which resazurin is reacted with the NADH or NADPH obtained in the 2-2 process and a diaphorase in the presence of an SH reagent, and the resulting fluorescence intensity or absorbance is measured.

Abstract translation: 在本发明的检测荧光或吸光度的方法中，在SH试剂和NADH或NADPH的存在下，心肌黄酶引起从青紫红素降低至芦荟碱，并且测量所得的荧光强度或吸光度。 本发明的ADP测定方法包括使葡萄糖与ADP和ADP依赖性己糖激酶反应的2-1工序，其中2-1工序中得到的葡萄糖-6-磷酸为 与NAD或NADP和葡萄糖-6-磷酸脱氢酶反应，和2-3次方法，其中天竺葵素与在2-2方法中获得的NADH或NADPH反应，在SH试剂存在下与心肌黄酶反应， 测量荧光强度或吸光度。

公开(公告)号：US20160251397A1

公开(公告)日：2016-09-01

申请号：US14949236

申请日：2015-11-23

Applicant: INDI MOLECULAR, INC.

Inventor： James R. Heath ,  Arundhati Nag ,  Samir Das ,  Kaycie M. Deyle ,  Steven Wesley Millward ,  Paul Edward Kearney

IPC: C07K7/02 ,  A61K51/08 ,  G01N33/574

CPC classification number: C07K7/02 ,  A61K38/00 ,  A61K38/08 ,  A61K49/0043 ,  A61K49/0056 ,  A61K51/08 ,  C07K14/001 ,  C12N15/1058 ,  C12Q1/485 ,  C12Q1/6804 ,  G01N33/573 ,  G01N33/57449 ,  G01N33/5748 ,  G01N33/57496 ,  G01N2333/91205 ,  G01N2333/91215

Abstract: The present application provides stable peptide-based Akt capture agents and the use thereof as detection, diagnosis, and treatment agents. The application further provides novel methods of developing stable peptide-based capture agents, including Akt capture agents, using iterative on-bead in situ click chemistry.

Abstract translation: 本申请提供稳定的基于肽的Akt捕获剂及其用作检测，诊断和治疗剂。 该应用进一步提供了使用迭代在珠粒原位点击化学开发稳定的基于肽的捕获剂（包括Akt捕获剂）的新方法。

公开(公告)号：US09383361B2

公开(公告)日：2016-07-05

申请号：US14548804

申请日：2014-11-20

Applicant: Biodesy, Inc.

Inventor： Joshua S. Salafsky

IPC: G01N33/573 ,  G01N33/68 ,  G01N33/74

CPC classification number: G01N33/54366 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/573 ,  G01N33/68 ,  G01N33/74 ,  G01N2021/6463 ,  G01N2333/726 ,  G01N2333/91205 ,  G01N2333/91215 ,  G01N2500/04

Abstract: The present invention discloses, inter alia, methods for labeling a target protein with an SHG-active probe for detection by second harmonic or sum-frequency generation in order to identify agents which bind to an allosteric site on the target protein thereby altering its structural conformation.