公开(公告)号：US20160160209A1

公开(公告)日：2016-06-09

申请号：US15045247

申请日：2016-02-17

Applicant: Kianoosh Peyvan

Inventor： Kianoosh Peyvan

IPC: C12N15/10 ,  C12Q1/68 ,  B01L3/00

CPC classification number: C12N15/101 ,  B01F15/0206 ,  B01F15/0223 ,  B01F15/0224 ,  B01F15/0233 ,  B01F2015/0221 ,  B01J4/02 ,  B01J2219/00177 ,  B01L3/502 ,  B01L3/527 ,  B01L2200/0631 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/049 ,  B01L2400/0638 ,  C12N15/1003 ,  C12Q1/6806

Abstract: A reagent delivery method includes placing a reagent delivery column including a plurality of reagent storage elements therein in a centrifugal device, coupling a breaching element to the housing, and rotating the centrifugal device to force the reagent storage elements toward the breaching element, breaching of the reagent storage elements releasing reagent therein and communicating the same to an external environment. A reagent delivery system includes a column having a housing, a plurality of reagent storage element sheets positioned in the housing or forming a stack, a plurality of breaching elements, and a microplate having a plurality of wells, the microplate coupled to the housing where each well corresponds or aligns with a breaching element and a reagent storage element. The system can be rotated in a centrifugal device to force the reagent storage element stacks toward the breaching elements.

Abstract translation: 试剂递送方法包括将包括多个试剂储存元件的试剂输送塔放置在离心装置中，将破裂元件连接到壳体，以及旋转离心装置以迫使试剂储存元件朝向破坏元件，破坏 试剂储存元件在其中释放试剂并将其传送到外部环境。 试剂递送系统包括具有壳体的柱，位于壳体中的多个试剂储存元件片或形成叠层，多个破裂元件和具有多个孔的微孔板，该微孔板联接到壳体，其中每个 很好地对应于或与违反元件和试剂储存元件对准。 该系统可以在离心装置中旋转以迫使试剂储存元件朝着破坏元件堆叠。

公开(公告)号：US20160152969A1

公开(公告)日：2016-06-02

申请号：US14951789

申请日：2015-11-25

Applicant: Seiko Epson Corporation

Inventor： Masato HANAMURA ,  Kotaro IDEGAMI ,  Yuji SAITO ,  Kiyohito YAMADA

IPC: C12N15/10

CPC classification number: C12N15/101 ,  B01L3/502 ,  B01L2200/025 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/042 ,  B01L2300/0838 ,  B01L2400/043 ,  C12N15/1003 ,  C12N15/1013 ,  G01N35/0098

Abstract: A nucleic acid extraction device includes a tube part in which a first plug of an oil, a second plug of a cleansing liquid not miscible with the oil for cleansing a material with adsorbed nucleic acid, a third plug of an oil, a fourth plug of an eluate not miscible with the oil for eluting nucleic acid from the material, and a fifth plug of an oil are provided in this order, and a cover part provided to surround the tube part.

Abstract translation: 核酸提取装置包括管部分，其中油的第一塞子，不与油混溶的清洁液的第二塞子，用于用吸附的核酸清洗材料，第三塞油塞，第四塞子 与油不混合的洗脱液，用于从材料中洗脱核酸，以及第五个油塞，依次设置有围绕管部的盖部。

公开(公告)号：US09315800B2

公开(公告)日：2016-04-19

申请号：US13963699

申请日：2013-08-09

Applicant: Industrial Technology Research Institute

Inventor： Yi-Chang Chen ,  Jane S-C Tsai

IPC: C07H21/00 ,  C12N15/10 ,  C07H1/06 ,  C07H1/08

CPC classification number: C12N15/1003 ,  C07H1/06 ,  C07H1/08 ,  C12N15/1013

Abstract: The disclosure provides a composition and method for isolating nucleic acids, in which the composition includes at least one halocarbon, at least one salt and at least one surfactant. Mixing the composition and a biosample to form a homogenized solution, nucleic acids in the solution can be easily isolated with a simple treatment and good yield.

Abstract translation: 本公开提供了用于分离核酸的组合物和方法，其中所述组合物包含至少一种卤化碳，至少一种盐和至少一种表面活性剂。 将组合物和生物样品混合以形成均质溶液，溶液中的核酸可以通过简单的处理和良好的产率容易地分离。

公开(公告)号：US20160032365A1

公开(公告)日：2016-02-04

申请号：US14776269

申请日：2014-03-13

Applicant: WM. WRIGLEY JR. COMPANY

Inventor： Amarnath MAITRA ,  David MORANDO

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/689 ,  C12N15/1003 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2600/158

Abstract: The invention is directed to methods of extracting nucleic acids from microorganisms or mammalian cells adhered to polymers that are malleable within a living organism and particularly malleable in the oral cavity of the living organism. The invention also provides for method of detecting and quantitating microorganisms that adhere to malleable polymers, such as chewing gum.

Abstract translation: 本发明涉及从附着于聚合物的微生物或哺乳动物细胞中提取核酸的方法，所述聚合物在活生物体内是可延展的并且特别是在活体的口腔中具有延展性。 本发明还提供了检测和定量粘附于可延展聚合物如口香糖的微生物的方法。

公开(公告)号：US20160030939A1

公开(公告)日：2016-02-04

申请号：US14767265

申请日：2014-02-11

Applicant: EPISTEM LIMITED

Inventor： Benjamin Cobb

IPC: B01L3/00 ,  C12N15/10

CPC classification number: B01L3/52 ,  B01L3/5023 ,  B01L2200/025 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0825 ,  B01L2300/126 ,  B01L2300/16 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0633 ,  C12N15/1003 ,  G01N1/28

Abstract: A sample preparation cartridge is described having a lower portion having four integrally-formed leaf springs, and a raised lower base part which is slightly below the level of the leaf springs. An upper portion of the cartridge engages with the lower portion, via four paired pins and holes formed in the lower and upper portions. The upper portion includes three well-shaped openings formed in the central region of the upper portion. Between the upper and lower portions is placed a sheet of sample preparation paper, resting on the leaf springs, which raise the lower face of the paper away from the raised lower base part, leaving a small air gap between the base and the paper. The upper face of the paper is in contact with the well-shaped openings of the upper portion. The user loads a liquid sample into the openings, and the paper wicks the liquid sample away from the loading location, leaving cellular debris in the place of application. Applying pressure to the upper portion of the cartridge urges the paper into contact with the raised base portion, so providing a firm base which allows the user to remove sections of the paper, using for example a biopsy needle, for further processing.

Abstract translation: 描述了一种样品制备盒，其具有具有四个一体形成的板簧的下部和稍微低于板簧高度的凸起的下部基部。 盒的上部通过形成在下部和上部中的四个成对销和孔与下部接合。 上部包括形成在上部中心区域的三个井形开口。 在上部和下部之间放置一张样品制备纸，放置在板簧上，使纸张的下表面远离升高的​​下部基部，在基部和纸张之间留下一个小的气隙。 纸张的上表面与上部的良好形状的开口接触。 用户将液体样品加载到开口中，并且纸将液体样品从装载位置吸出，从而将细胞碎片留在施加位置。 向盒的上部施加压力促使纸与升高的基部接触，从而提供牢固的基部，其允许使用者例如使用活检针去除纸张的部分用于进一步处理。

公开(公告)号：US20150376601A1

公开(公告)日：2015-12-31

申请号：US14704580

申请日：2015-05-05

Applicant: Diagon Ltd.

Inventor： Gabor Kiss ,  Janos Kiss ,  Katalin Sztancsik Ambrusne Kovacs ,  Georgina Bernath

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  C07H21/00 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q2523/32

Abstract: Procedure for the specific isolation of total DNA content of bacterial germs of different samples, in the course of which the cells are lysated, the DNA content of the lysate is bound selectively, it is washed and then the desalinated linear polymer nucleic acid is eluted from the binding surface in an aqueous solution. Before cell lysis the nonviable bacterial cells are separated from the viable cells on the basis of their different cell surface physical-chemical characteristics, the viable cells of the sample are kept and then lysated using a mechanical and/or enzymatic, favorably lysozyme enzymatic method. After this exclusively double-stranded DNA deriving from the lysate of viable cells is bound on a —SiO2—TiO2— matrix containing chemically activated —OH and dodecylamine groups, and after washing it, the desalinated linear polymer nucleic acid is eluted in an aqueous solution.

Abstract translation: 特异性分离不同样品的细菌细菌的总DNA含量的步骤，在细胞裂解过程中，裂解物的DNA含量选择性地结合，洗涤，然后脱盐的线性聚合物核酸从 在水溶液中的结合表面。 在细胞裂解之前，根据不同的细胞表面物理化学特性，将不可维护的细菌细胞从活细胞中分离出来，保存样品的活细胞，然后使用机械和/或酶促的溶菌酶酶法进行裂解。 之后，将衍生自活细胞裂解物的纯双链DNA结合在含有化学活化的-OH和十二烷基胺基团的-SiO 2 -TiO 2基质上，并且在洗涤之后，脱盐的线性聚合物核酸在水溶液 。

公开(公告)号：US09174210B2

公开(公告)日：2015-11-03

申请号：US13025923

申请日：2011-02-11

Applicant: Richard F. Selden ,  Eugene Tan

Inventor： Richard F. Selden ,  Eugene Tan

IPC: B01L3/00 ,  C12N15/10 ,  B01L7/00

CPC classification number: B01L3/502753 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/50825 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/043 ,  B01L2400/0487 ,  C12N15/1003 ,  C12N15/101

Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.

Abstract translation: 用于从仪器使用的未处理的样品装置分离核酸，细胞裂解物和细胞悬浮液的独立装置包括至少一个输入，和：（i）大流体组分，包括用于接收未处理样品的室 从收集装置和至少一个填充的液体净化试剂储存容器; 和（ii）通过至少一个微流体元件与所述宏流体组分连通的微流体组分，所述微流体组分还包含至少一个核酸纯化基质; 和（iii）通过微流体元件和核酸纯化基质的至少一个界面端口连接到仪器上的用于驱动所述液体纯化试剂的驱动机构的接口端口，其中所述设备的唯一输入通过所述室和所述接口端口 驱动机构。

公开(公告)号：US20150307911A1

公开(公告)日：2015-10-29

申请号：US14794048

申请日：2015-07-08

Applicant: Boehringer lngelheim RCV GmbH & Co. KG

Inventor： Jochen URTHALER ,  Christine ASCHER ,  Daniel BUCHELI

IPC: C12P19/34 ,  C12N15/10

CPC classification number: C12P19/34 ,  B03D1/028 ,  B03D1/082 ,  B03D1/1462 ,  B03D1/1468 ,  B03D1/1487 ,  B03D1/18 ,  B03D2203/003 ,  C12N15/1003 ,  C12N15/1006

Abstract: A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).

Abstract translation: 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。

公开(公告)号：US09163275B2

公开(公告)日：2015-10-20

申请号：US13896778

申请日：2013-05-17

Applicant: Expression Pathology, Inc.

Inventor： Marlene M. Darfler ,  David B. Krizman

IPC: C12Q1/68 ,  C12M3/00 ,  G01N33/53 ,  C12Q1/37 ,  C12N15/10 ,  G01N1/44 ,  C40B30/04 ,  C40B40/06 ,  C40B40/08 ,  G01N1/30 ,  G01N1/40

CPC classification number: G01N1/4044 ,  C12N15/1003 ,  C12Q1/37 ,  G01N1/30 ,  G01N1/44

Abstract: The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

Abstract translation: 本发明提供了一种将组织病理学处理的生物样品，组织和细胞直接转化成多用途生物分子裂解物的方法。 该方法允许同时提取，分离，溶解和储存包含在组织病理学处理的生物样品中的所有生物分子，从而形成所述样品的代表性文库。 这种多用途生物分子裂解物是可稀释的，可溶的，能够分级，并用于任何数量的后续实验。

公开(公告)号：US09163228B2

公开(公告)日：2015-10-20

申请号：US13639808

申请日：2011-04-08

Applicant: Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

Inventor： Roland Fabis ,  Markus Müller ,  Jörg Hucklenbroich ,  Mario Scherer

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  C12N15/101

Abstract: The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

Abstract translation: 本发明涉及用于分离和纯化来自生物样品的核酸的优选包含基因组DNA的方法，包括以下步骤：使用包含阴离子表面活性剂离子源的裂解缓冲液裂解样品，任选地分解裂解物中存在的RNA， 从裂解物沉淀表面活性剂离子，并通过尺寸排阻色谱法将沉淀物和其它污染物分离出来。 本发明还涉及裂解缓冲液，裂解细胞的方法和用于分离和纯化核酸的试剂盒。