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    SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS
    72.
    发明申请
    SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS 有权

    公开(公告)号：US20210262020A1

    公开(公告)日：2021-08-26

    申请号：US17174519

    申请日：2021-02-12

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Darren Roy Link

    IPC: C12Q1/6848 ,  G01N1/38 ,  C12Q1/686 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6827

    Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

    Multiple beads per droplet resolution
    73.
    发明授权
    Multiple beads per droplet resolution 有权

    公开(公告)号：US11097247B2

    公开(公告)日：2021-08-24

    申请号：US16911961

    申请日：2020-06-25

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Ronald Lebofsky ,  Nicholas Heredia

    IPC: B01J19/00 ,  C12Q1/6806

    Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.

    Amplification reporter with base-pairing oligomers
    74.
    发明授权
    Amplification reporter with base-pairing oligomers 有权

    公开(公告)号：US11085070B2

    公开(公告)日：2021-08-10

    申请号：US16834854

    申请日：2020-03-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    ELECTRONIC COMPONENT ASSEMBLY WITH THERMALLY CONDUCTIVE STRUCTURES FOR IMAGE SENSORS
    75.
    发明申请
    ELECTRONIC COMPONENT ASSEMBLY WITH THERMALLY CONDUCTIVE STRUCTURES FOR IMAGE SENSORS 有权

    公开(公告)号：US20210176386A1

    公开(公告)日：2021-06-10

    申请号：US17108672

    申请日：2020-12-01

    Applicant: BIO-RAD LABORATORIES, INC.

    Inventor： Stephen Swihart ,  Li Lu ,  Wade Jameson ,  Evan Thrush ,  Michael Griffin ,  Evelio Perez

    IPC: H04N5/225 ,  H05K7/20

    Abstract: An electronic component assembly having thermal pads with thermal vias coupling an image sensor and a camera board fab is provided for heat dissipation. The electronic component assembly can include: a circuit board having at least one thermal pad disposed on a top surface of the circuit board; and an image sensor disposed on the top surface of the circuit board, having at least one conductive pad disposed at at least one corner of the image sensor. The at least one thermal pad is coupled to the at least one conductive pad of the image sensor and the at least one thermal pad is formed with a plurality of first thermal vias penetrating the thermal pad and the circuit board for transfer of heat of the image sensor.

    SAMPLE PROCESSING BARCODED BEAD COMPOSITION, METHOD, MANUFACTURING, AND SYSTEM
    76.
    发明申请
    SAMPLE PROCESSING BARCODED BEAD COMPOSITION, METHOD, MANUFACTURING, AND SYSTEM 有权

    公开(公告)号：US20210171939A1

    公开(公告)日：2021-06-10

    申请号：US17109704

    申请日：2020-12-02

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Adam McCoy ,  Kalyan Handique ,  Jonathan Bell ,  Vishal Sharma ,  Sida Wang

    IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/686

    Abstract: An embodiment of a composition for target material separation includes: a body and one or more molecules coupled to the body and structured for functionalization of the composition. In embodiments, each of the one or more molecules can include one or more of: a linker region; a polymerase chain reaction (PCR) segment or oligo binding region; one or more barcode region(s); a unique molecule identifier; a preparation-facilitating segment; an active segment; and a molecular scissor or cleavage region, wherein various regions can be coupled together in order to provide functionality to the composition. The invention(s) also cover manufacturing of the composition and various applications of use, in the context of target material capture (e.g., from single cells or other biological material).

    MODIFICATION OF SURFACE PROPERTIES OF MICROFLUIDIC DEVICES
    78.
    发明申请
    MODIFICATION OF SURFACE PROPERTIES OF MICROFLUIDIC DEVICES 有权

    公开(公告)号：US20210162406A1

    公开(公告)日：2021-06-03

    申请号：US17153311

    申请日：2021-01-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Joshua RITCHEY ,  Yi XUE ,  Robert MELTZER

    IPC: B01L3/00 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00

    Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

    METHOD AND SYSTEM FOR SAMPLING MATERIAL FROM CELLS
    79.
    发明申请
    METHOD AND SYSTEM FOR SAMPLING MATERIAL FROM CELLS 有权

    公开(公告)号：US20210155975A1

    公开(公告)日：2021-05-27

    申请号：US17105333

    申请日：2020-11-25

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Arkadiusz BIBILLO ,  Pranav PATEL ,  Christopher REGGIARDO ,  Jonathan PETERSEN

    IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6853 ,  C12N15/10

    Abstract: Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.

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