公开(公告)号：US12221634B2

公开(公告)日：2025-02-11

申请号：US18449107

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20220235395A1

公开(公告)日：2022-07-28

申请号：US17461348

申请日：2021-08-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12Q1/686 ,  C12N9/12 ,  C12Q1/6844 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/6806

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US12221633B2

公开(公告)日：2025-02-11

申请号：US18348544

申请日：2023-07-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20240124856A1

公开(公告)日：2024-04-18

申请号：US18449107

申请日：2023-08-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US11739307B2

公开(公告)日：2023-08-29

申请号：US17055801

申请日：2019-05-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US20210207109A1

公开(公告)日：2021-07-08

申请号：US17055801

申请日：2019-05-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US12163164B2

公开(公告)日：2024-12-10

申请号：US16928915

申请日：2020-07-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12 ,  C12N1/20 ,  C12R1/01 ,  C40B40/06

Abstract: Hybrid reverse transcriptases are provided that comprise a non-retroviral retrotransposon, or a fragment of the non-retroviral retrotransposon having reverse transcriptase activity, joined to a nucleic acid binding protein. Also provided are methods of using the hybrid reverse transcriptases to prepare a cDNA molecule library.

公开(公告)号：US20240026316A1

公开(公告)日：2024-01-25

申请号：US18348544

申请日：2023-07-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

CPC classification number: C12N9/1276 ,  C12Y207/07049 ,  C07K2319/20

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US10954557B2

公开(公告)日：2021-03-23

申请号：US16168002

申请日：2018-10-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12Q1/686 ,  C12N9/12 ,  C12Q1/6844 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/6806

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US11542541B2

公开(公告)日：2023-01-03

申请号：US17105333

申请日：2020-11-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel ,  Christopher Reggiardo ,  Jonathan Petersen

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6853 ,  C12Q1/6874

Abstract: Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.