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    Amplification reporter with base-pairing oligomers
    2.
    发明授权
    Amplification reporter with base-pairing oligomers 有权

    公开(公告)号：US11085070B2

    公开(公告)日：2021-08-10

    申请号：US16834854

    申请日：2020-03-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    Amplification reporter with base-pairing oligomers
    4.
    发明授权
    Amplification reporter with base-pairing oligomers 有权

    公开(公告)号：US10604789B2

    公开(公告)日：2020-03-31

    申请号：US15411482

    申请日：2017-01-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    Amplification reporter with base-pairing oligomers
    6.
    发明授权
    Amplification reporter with base-pairing oligomers 有权
    扩增报告基因配对低聚物

    公开(公告)号：US09556475B2

    公开(公告)日：2017-01-31

    申请号：US14457863

    申请日：2014-08-12

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。

    AMPLIFICATION ASSAY WITH A PROBE COMPETITOR
    7.
    发明申请
    AMPLIFICATION ASSAY WITH A PROBE COMPETITOR 有权
    具有探测器竞争者的放大测试

    公开(公告)号：US20160362731A1

    公开(公告)日：2016-12-15

    申请号：US15181173

    申请日：2016-06-13

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Samantha Cooper ,  Wei Yang ,  Jennifer Berman

    IPC: C12Q1/68 ,  G06F19/22

    CPC classification number: C12Q1/6827 ,  G06F19/22 ,  C12Q2537/16 ,  C12Q2545/107

    Abstract: Methods and compositions for detecting an allelic form of a target. In an exemplary method, partitions may be created that collectively contain at least one first allelic form and a second allelic form of a target. Each partition may contain (i) a same probe capable of binding specifically to each of the first and second allelic forms of the target and (ii) a competitor configured to bind selectively to the second allelic form relative to the first allelic form and to block binding of the probe to the second allelic form. The first allelic form of the target may be amplified in the partitions. A signal may be detected from a label of the probe while the label is contained by the partitions. A number of partitions that are positive (or negative) for the at least one first allelic form may be determined based on the signal.

    Abstract translation: 用于检测靶标的等位基因形式的方法和组合物。 在示例性方法中，可以创建共同包含目标的至少一个第一等位基因形式和第二等位基因形式的分区。 每个分区可以包含（i）能够特异性结合靶的第一和第二等位基因形式中的每一个的相同探针和（ii）被配置为相对于第一等位基因形式选择性结合第二等位基因形式的竞争物并阻断 探针与第二等位基因形式的结合。 目标的第一个等位基因形式可以在分区中扩增。 当标签被分区包含时，可以从探针的标签检测信号。 可以基于该信号确定对于至少一个第一等位基因形式为正（或负）的多个分区。

    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
    8.
    发明申请
    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS 有权
    放大报告器与基本配对的低分辨率

    公开(公告)号：US20150148250A1

    公开(公告)日：2015-05-28

    申请号：US14457863

    申请日：2014-08-12

    Applicant: Bio-Rad laboratories, Inc.

    Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

    IPC: C12Q1/68

    CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。

    Amplification assay with a probe competitor
    10.
    发明授权
    Amplification assay with a probe competitor 有权

    公开(公告)号：US09868981B2

    公开(公告)日：2018-01-16

    申请号：US15181173

    申请日：2016-06-13

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Samantha Cooper ,  Wei Yang ,  Jennifer Berman

    IPC: C12Q1/68 ,  G06F19/22

    CPC classification number: C12Q1/6827 ,  G06F19/22 ,  C12Q2537/16 ,  C12Q2545/107

    Abstract: Methods and compositions for detecting an allelic form of a target. In an exemplary method, partitions may be created that collectively contain at least one first allelic form and a second allelic form of a target. Each partition may contain (i) a same probe capable of binding specifically to each of the first and second allelic forms of the target and (ii) a competitor configured to bind selectively to the second allelic form relative to the first allelic form and to block binding of the probe to the second allelic form. The first allelic form of the target may be amplified in the partitions. A signal may be detected from a label of the probe while the label is contained by the partitions. A number of partitions that are positive (or negative) for the at least one first allelic form may be determined based on the signal.

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