公开(公告)号：US12024737B2

公开(公告)日：2024-07-02

申请号：US17396148

申请日：2021-08-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q1/6818 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q1/6823 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.

公开(公告)号：US11085070B2

公开(公告)日：2021-08-10

申请号：US16834854

申请日：2020-03-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

公开(公告)号：US10604789B2

公开(公告)日：2020-03-31

申请号：US15411482

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

公开(公告)号：US20170166957A1

公开(公告)日：2017-06-15

申请号：US15411482

申请日：2017-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

公开(公告)号：US09556475B2

公开(公告)日：2017-01-31

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。

公开(公告)号：US20150148250A1

公开(公告)日：2015-05-28

申请号：US14457863

申请日：2014-08-12

Applicant: Bio-Rad laboratories, Inc.

Inventor： John F. Regan ,  Dianna Maar ,  Dawne N. Shelton ,  Tina C. Legler ,  Samantha Cooper ,  Wei Yang ,  Eli A. Hefner ,  Niels Klitgord

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2527/107 ,  C12Q2565/1015 ,  C12Q2537/1373 ,  C12Q2545/107 ,  C12Q2565/101

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract translation: 用于使用扩增报道子进行扩增测定的系统，包括方法，装置和组合物，所述扩增报告基团包括能够在报道分子的熔点以下彼此碱基配对的第一低聚物和第二低聚物。 报告物可能具有受到影响的可检测的光致发光，例如通过第一和第二寡聚体彼此的碱基配对而减少。 可以在高于熔解温度的至少一个体积（例如多个分区）中扩增靶，例如核酸靶序列，并且可以从低于熔解温度的至少一个体积检测报告物的光致发光 。 可以基于检测到的光致发光来确定靶的特性，例如靶的浓度。