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公开(公告)号：US20160102346A1
公开(公告)日：2016-04-14
申请号：US14977573
申请日：2015-12-21
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Svilen S. Tzonev , Yann Jouvenot , Claudia Litterst , Dianna Maar , Geoff McDermott
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q2537/143 , C12Q2537/165 , C12Q2545/107
Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.
Abstract translation: 数字测定系统，包括方法，装置和组合物，用于在每个含有通用报道分子和用于靶扩增的特异性报道分子的分区中进行靶分析。
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公开(公告)号：US20140221238A1
公开(公告)日：2014-08-07
申请号：US14171761
申请日：2014-02-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Svilen S. Tzonev , Yann Jouvenot , Claudia Litterst , Dianna Maar , Geoff McDermott
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q2537/143 , C12Q2537/165 , C12Q2545/107
Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.
Abstract translation: 数字测定系统，包括方法，装置和组合物，用于在每个含有通用报道分子和用于靶扩增的特异性报道分子的分区中进行靶分析。
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公开(公告)号：US12024737B2
公开(公告)日：2024-07-02
申请号：US17396148
申请日：2021-08-06
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC: C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
CPC classification number: C12Q1/6818 , C12Q1/6823 , C12Q1/6848 , C12Q1/6818 , C12Q2527/107 , C12Q2565/1015 , C12Q1/6823 , C12Q2537/1373 , C12Q2545/107 , C12Q2565/101
Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.
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公开(公告)号：US11085070B2
公开(公告)日：2021-08-10
申请号：US16834854
申请日：2020-03-30
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC: C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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公开(公告)号：US10167509B2
公开(公告)日：2019-01-01
申请号：US14848311
申请日：2015-09-08
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/68 , C07H21/00 , C12Q1/6883 , C12Q1/683 , C12Q1/6858
Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.
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Patent Agency Ranking
公开(公告)号：US09845496B2
公开(公告)日：2017-12-19
申请号：US14977573
申请日：2015-12-21
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Svilen S. Tzonev , Yann Jouvenot , Claudia Litterst , Dianna Maar , Geoff McDermott
CPC classification number: C12Q1/6851 , C12Q2537/143 , C12Q2537/165 , C12Q2545/107
Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.
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公开(公告)号：US11499181B2
公开(公告)日：2022-11-15
申请号：US16235996
申请日：2018-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, Jr.
IPC: C12Q1/6858 , C12Q1/683 , C12Q1/6883 , C12Q1/6806 , C12Q1/6827 , C12Q1/686
Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US20190241947A1
公开(公告)日：2019-08-08
申请号：US16235996
申请日：2018-12-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Serge Saxonov , Michael Y. Lucero , Benjamin J. Hindson , Phillip Belgrader , Simant Dube , Austin P. So , Jeffrey C. Mellen , Nicholas J. Heredia , Kevin D. Ness , Billy W. Colston, JR.
IPC: C12Q1/6858 , C12Q1/6806 , C12Q1/686 , C12Q1/6827
CPC classification number: C12Q1/6806 , C12Q1/6827 , C12Q1/683 , C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2535/125 , C12Q2600/158 , C12Q2600/172 , C12Q2537/16 , C12Q2563/159 , C12Q2537/165
Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.
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公开(公告)号：US09217175B2
公开(公告)日：2015-12-22
申请号：US14171761
申请日：2014-02-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Svilen S. Tzonev , Yann Jouvenot , Claudia Litterst , Dianna Maar , Geoff McDermott
CPC classification number: C12Q1/6851 , C12Q2537/143 , C12Q2537/165 , C12Q2545/107
Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.
Abstract translation: 数字测定系统，包括方法，装置和组合物，用于在每个含有通用报道分子和用于靶扩增的特异性报道分子的分区中进行靶分析。
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公开(公告)号：US10604789B2
公开(公告)日：2020-03-31
申请号：US15411482
申请日：2017-01-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： John F. Regan , Dianna Maar , Dawne N. Shelton , Tina C. Legler , Samantha Cooper , Wei Yang , Eli A. Hefner , Niels Klitgord
IPC: C12Q1/68 , C12Q1/6818 , C12Q1/6823 , C12Q1/6848
Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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