公开(公告)号：US09970052B2

公开(公告)日：2018-05-15

申请号：US14191295

申请日：2014-02-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Duc Do ,  Claudia Litterst ,  Dianna Maar

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/686 ,  C12Q1/6844 ,  G01N21/6408

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US09217175B2

公开(公告)日：2015-12-22

申请号：US14171761

申请日：2014-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Svilen S. Tzonev ,  Yann Jouvenot ,  Claudia Litterst ,  Dianna Maar ,  Geoff McDermott

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2545/107

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Abstract translation: 数字测定系统，包括方法，装置和组合物，用于在每个含有通用报道分子和用于靶扩增的特异性报道分子的分区中进行靶分析。

公开(公告)号：US20240279730A1

公开(公告)日：2024-08-22

申请号：US18654169

申请日：2024-05-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Duc Do ,  Claudia Litterst ,  Dianna Maar

IPC: C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

CPC classification number: C12Q1/686 ,  C12Q1/6844 ,  G01N21/6408

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US20150225786A1

公开(公告)日：2015-08-13

申请号：US14622515

申请日：2015-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Svilen Tzonev ,  Jeremy Agresti

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q2521/313 ,  C12Q2523/101 ,  C12Q2563/179

Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Abstract translation: 提供方法用于在分区中进行染色质或染色体构象捕获测定的组合物和试剂盒。

公开(公告)号：US20140106359A1

公开(公告)日：2014-04-17

申请号：US14054698

申请日：2013-10-15

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Claudia Litterst ,  Ryan Thane Koehler

IPC: C12Q1/48

CPC classification number: C12Q1/48 ,  C12Q1/6844 ,  C12Q2521/113

Abstract: Digital assay system, including methods and apparatus, for determining a presence or activity of telomerase in a sample.

Abstract translation: 用于确定样品中端粒酶的存在或活性的数字测定系统，包括方法和装置。

公开(公告)号：US09702822B2

公开(公告)日：2017-07-11

申请号：US13973940

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Austin P. So ,  Duc Do

IPC: C12Q1/68 ,  C12P19/34 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2525/204 ,  C12Q2563/159

Abstract: Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

公开(公告)号：US09347094B2

公开(公告)日：2016-05-24

申请号：US14171393

申请日：2014-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Luis A. Ugozzoli

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/6844 ,  C12Q2537/16 ,  C12Q2563/159

Abstract: A method of characterizing telomere length. A template may be synthesized in a bulk phase reaction mixture containing genomic DNA. Partitions may be formed after synthesizing the template in the bulk phase reaction mixture, with only a subset of the partitions containing at least one copy of the template. At least a region of the template may be amplified in partitions. Amplification data may be collected from partitions. A measure of telomere length may be determined for the genomic DNA based on the amplification data. In some embodiments, the bulk phase reaction mixture may be thermally cycled for at least two cycles, and heated to a denaturation temperature and cooled to an annealing temperature in each cycle.

Abstract translation: 一种表征端粒长度的方法。 可以在含有基因组DNA的本体相反应混合物中合成模板。 在本体相反应混合物中合成模板之后可以形成分隔，只有分区的一个子集包含至少一个模板副本。 模板的至少一个区域可以在分区中被放大。 可以从分区收集扩增数据。 可以基于扩增数据确定基因组DNA的端粒长度的量度。 在一些实施方案中，本体相反应混合物可以热循环至少两个循环，并加热至变性温度并在每个循环中冷却至退火温度。

公开(公告)号：US11377684B2

公开(公告)日：2022-07-05

申请号：US15973243

申请日：2018-05-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Duc Do ,  Claudia Litterst ,  Dianna Maar

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US09845496B2

公开(公告)日：2017-12-19

申请号：US14977573

申请日：2015-12-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Svilen S. Tzonev ,  Yann Jouvenot ,  Claudia Litterst ,  Dianna Maar ,  Geoff McDermott

IPC: C07H21/04 ,  C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2545/107

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

公开(公告)号：US09523116B2

公开(公告)日：2016-12-20

申请号：US14171754

申请日：2014-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Svilen S. Tzonev ,  Claudia Litterst

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2545/107

Abstract: Digital assay system, including methods and apparatus, for calculating a level of one or more targets. In an exemplary method, data for amplification of a plurality of targets including a first target may be collected from partitions. A level of the first target may be calculated from only a subset of the data that excludes partitions according to target content.

Abstract translation: 数字测定系统，包括用于计算一个或多个目标的水平的方法和装置。 在示例性方法中，可以从分区收集用于放大包括第一目标的多个目标的数据。 可以仅从根据目标内容排除分区的数据的子集计算第一目标的级别。