公开(公告)号：US08298803B2

公开(公告)日：2012-10-30

申请号：US13269887

申请日：2011-10-10

Applicant: Michelle Maranta ,  Kimberly Brown ,  James Langston

Inventor： Michelle Maranta ,  Kimberly Brown ,  James Langston

IPC: C12N9/24 ,  C12N1/20 ,  C12N15/00 ,  C12N5/10 ,  C12N5/00 ,  C07H21/04

CPC classification number: C12P19/14 ,  C12N9/2402 ,  C12N15/8245 ,  C12N15/8246 ,  C12N2310/14 ,  C12Y302/01055

Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有α-L-阿拉伯呋喃糖苷酶活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：US20120266328A1

公开(公告)日：2012-10-18

申请号：US13354503

申请日：2012-01-20

Applicant: KEVIN A. GRAY ,  LISHAN ZHAO ,  MICHELLE H. CAYOUETTE

Inventor： KEVIN A. GRAY ,  LISHAN ZHAO ,  MICHELLE H. CAYOUETTE

IPC: C12N9/42 ,  C12N1/21 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N15/867 ,  C12N15/861 ,  C12N15/864 ,  C12N5/07 ,  C12N9/26 ,  C12N11/00 ,  C12Q1/34 ,  G01N33/573 ,  C12P19/34 ,  C12N15/01 ,  C40B50/06 ,  C12P19/14 ,  C40B30/08 ,  C12Q1/02 ,  C12N9/96 ,  C12N15/82 ,  C12P19/00 ,  C12P7/10 ,  C12S3/00 ,  C12N15/56 ,  A01K67/027 ,  A01H5/00 ,  A01H5/10 ,  C07H21/00 ,  C07H21/02 ,  C07H1/00 ,  A01H1/06 ,  A61K9/14 ,  A61K38/47 ,  A23L1/48 ,  C10L1/222 ,  A21D2/08 ,  C12G3/00 ,  C12C7/00 ,  C12C11/00 ,  A23K1/18 ,  A23C9/12 ,  A23C19/00 ,  A23G9/38 ,  A23C23/00 ,  D21H17/05 ,  D21H17/22 ,  C12N15/63

CPC classification number: C12N9/2437 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/248 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01023 ,  C12Y302/01039 ,  C12Y302/01055 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17 ,  Y10T428/249921

Abstract: The invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

Abstract translation: 本发明涉及具有任何纤维素分解活性的多肽，例如纤维素酶活性，例如内切葡聚糖酶，纤维二糖水解酶，β-葡糖苷酶，木聚糖酶，甘露聚糖，β-氧化酶，阿拉伯呋喃糖苷酶和/或低聚酶活性，包括耐热和耐热活性， 和编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽可用于各种药物，农业，食品和饲料加工以及工业环境中。 本发明还提供包含本发明的至少一种酶的酶的混合物的组合物或制品。

公开(公告)号：US20120028334A1

公开(公告)日：2012-02-02

申请号：US13269887

申请日：2011-10-10

Applicant: Michelle Maranta ,  Kimberly Brown ,  James Langston

Inventor： Michelle Maranta ,  Kimberly Brown ,  James Langston

IPC: C12N9/24 ,  C12N15/63 ,  C12N1/19 ,  C12N1/21 ,  C12N1/15 ,  C12N5/10 ,  C12N15/56 ,  A01H5/00

CPC classification number: C12P19/14 ,  C12N9/2402 ,  C12N15/8245 ,  C12N15/8246 ,  C12N2310/14 ,  C12Y302/01055

Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20110136175A1

公开(公告)日：2011-06-09

申请号：US12927504

申请日：2010-11-15

Applicant: David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily Henriksen

Inventor： David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily Henriksen

IPC: C12P19/00 ,  C07H21/04 ,  C12N9/00 ,  C12N9/42 ,  C07K14/00

CPC classification number: C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

公开(公告)号：US20090099079A1

公开(公告)日：2009-04-16

申请号：US12205694

申请日：2008-09-05

Applicant: Mark A. Emalfarb ,  Alexander Vasilievich Gusakov ,  Peter J. Punt ,  Jan Cornelis Verdoes ,  Arkady Panteleimonovich Sinitsyn ,  Elena Vlasenko ,  Sandra Wihelmina Agnes Hinz ,  Mark Gosink ,  Zhijie Jiang ,  Jacoba Van der Meij

Inventor： Mark A. Emalfarb ,  Alexander Vasilievich Gusakov ,  Peter J. Punt ,  Jan Cornelis Verdoes ,  Arkady Panteleimonovich Sinitsyn ,  Elena Vlasenko ,  Sandra Wihelmina Agnes Hinz ,  Mark Gosink ,  Zhijie Jiang ,  Jacoba Van der Meij

IPC: A61K38/16 ,  C07H21/00 ,  C07K14/00 ,  C07K16/44 ,  C12N9/42 ,  C12N15/64 ,  C12N5/10 ,  C12N1/15 ,  C12N1/13 ,  C12N1/21 ,  C12P21/00 ,  C12N9/26 ,  C12N9/24 ,  C12N9/20 ,  C12N9/16 ,  C12P19/02 ,  C12P7/04 ,  C12P7/06 ,  D06L3/11 ,  C09B67/42 ,  C12S11/00 ,  C11D7/42 ,  D06Q1/02 ,  A23K1/165 ,  A23L1/48 ,  A61P29/00 ,  C12N1/06 ,  C12P13/04 ,  C12P7/26

CPC classification number: C12N9/248 ,  C07H21/00 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2434 ,  C12N9/2471 ,  C12Y301/01072 ,  C12Y302/01023 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01139 ,  C12Y302/01151 ,  C12Y302/01155 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.

Abstract translation: 本发明涉及新型酶及其生产方法。 更具体地，本发明涉及各种真菌酶。 描述了编码这种酶，组合物，重组和遗传修饰的宿主细胞的核酸分子和使用方法。 本发明还涉及将木质纤维素生物质转化成可降解木质纤维素材料的酶和酶的新组合（包括提供来自植物生物质的糖的协同释放的酶）的方法。 本发明还涉及通过细胞壁降解释放细胞含量的方法。 本发明还涉及在各种其它方法中使用新型酶和这些酶的组合物的方法，包括洗涤衣物，洗涤剂方法，生物精制，纸和纸浆的脱墨和生物漂白以及废物流的处理。

公开(公告)号：US6066356A

公开(公告)日：2000-05-23

申请号：US170354

申请日：1998-10-13

Applicant: Monique Josina Andrea Van Der Wouw ,  Albert Johannes Joseph Van Ooijen ,  Marcus Matheus Catharina Gielkens ,  Leendert Hendrik De Graaff ,  Jacob Visser

Inventor： Monique Josina Andrea Van Der Wouw ,  Albert Johannes Joseph Van Ooijen ,  Marcus Matheus Catharina Gielkens ,  Leendert Hendrik De Graaff ,  Jacob Visser

IPC: A21D20060101 ,  A21D8/04 ,  A21D8/06 ,  A23G1/00 ,  A23K20060101 ,  A23K1/165 ,  C07H21/04 ,  C12N20060101 ,  C12N1/00 ,  C12N1/14 ,  C12N1/15 ,  C12N5/10 ,  C12N9/00 ,  C12N9/24 ,  C12N9/42 ,  C12N11/00 ,  C12N15/11 ,  C12N15/55 ,  C12R1/55 ,  C12R1/66 ,  C12S3/02 ,  D21C3/00 ,  A23G2/00

CPC classification number: C12Y302/01055 ,  C12N9/2402 ,  C12R1/66 ,  Y10S435/913 ,  Y10S435/917

Abstract: An arabinoxylan-degrading enzyme from Aspergillus is provided. The enzyme is active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.

Abstract translation: 提供来自曲霉的阿糖木聚糖降解酶。 该酶在从玉米获得的水不溶性固体的降解中是有活性的。 该酶可用于制备动物饲料组合物，人类食物或工业过程。

公开(公告)号：US5989887A

公开(公告)日：1999-11-23

申请号：US914848

申请日：1997-08-18

Applicant: Margaretha Van Heuvel ,  Janna Gardina Bakhuis ,  Yves Coutel ,  Abraham Harder ,  Leendert Hendrik De Graaff ,  Michel Johannes Anthonie Flipphi ,  Peter Van Der Veen ,  Jacob Visser ,  Peter Michael Andreoli

Inventor： Margaretha Van Heuvel ,  Janna Gardina Bakhuis ,  Yves Coutel ,  Abraham Harder ,  Leendert Hendrik De Graaff ,  Michel Johannes Anthonie Flipphi ,  Peter Van Der Veen ,  Jacob Visser ,  Peter Michael Andreoli

IPC: C12N9/24 ,  C12N15/56 ,  C12N15/80 ,  C12N15/74

CPC classification number: C12Y302/01108 ,  C12N15/80 ,  C12N9/2402 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01055 ,  C12Y302/01099

Abstract: The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.

Abstract translation: 本发明提供从编码具有阿拉伯聚糖降解活性的酶的真菌获得的纯化和分离的DNA分子的克隆和表达。 本发明还提供含有这些DNA分子的DNA构建体及其在选定的微生物宿主细胞中增强表达的方法。 本发明进一步提供了使用如此制备的阿拉伯聚糖降解酶的方法。

公开(公告)号：US5489526A

公开(公告)日：1996-02-06

申请号：US418331

申请日：1995-04-06

Applicant: Bruce L. Zamost ,  Dana D. Elm

Inventor： Bruce L. Zamost ,  Dana D. Elm

IPC: C12N9/24 ,  C12N1/00 ,  C12N1/20

CPC classification number: C12Y302/01032 ,  C12N9/2482 ,  C12Y302/01008 ,  C12Y302/01037 ,  C12Y302/01055 ,  Y10S435/832

Abstract: An isolated xylosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The xylosidase has a maximum activity at about pH 6.0 and at about 75.degree. C., maintains at least about 60% of its maximum activity at about 65.degree. C. and pH 7 after 4 hours, is resistant to end-product inhibition maintaining over 75% of maximum activity in the presence of 1 molar xylose and has an isoelectric point of about 5.0. The xylosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60.degree. C. and a pH of at least about 7.0. The xylosidase is used along with at least two xylanases and an arabinofuranosidase isolated from the above Bacillus stearothermophilus strains.

Abstract translation: 公开了来自嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661的分离的木糖苷酶。 木糖苷酶在约pH6.0和约75℃下具有最大活性，在约65℃保持其最大活性的至少约60％，4小时后保持pH7，耐受最终产物抑制 在1摩尔木糖存在下具有75％的最大活性，并具有约5.0的等电点。 木糖苷酶可用于在至少约60℃的温度和至少约7.0的pH下水解木浆中存在的木聚糖的方法。 木糖苷酶与至少两种木聚糖酶和从上述嗜热脂肪芽孢杆菌菌株分离的阿拉伯呋喃糖苷酶一起使用。

公开(公告)号：US20190032037A1

公开(公告)日：2019-01-31

申请号：US15742892

申请日：2016-07-08

Applicant: International Centre For Genetic Engineering & Biotechnology ,  Department of Biotechnology Ministry of Science & Technology

Inventor： Syed Shams YAZDANI ,  Funso Emmanuel OGUMOLU

IPC: C12N9/24 ,  C12N9/18 ,  C12N9/42 ,  C12N9/34

CPC classification number: C12N9/2402 ,  C12N9/18 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12P2203/00 ,  C12Y301/01072 ,  C12Y302/01 ,  C12Y302/01003 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01055 ,  C12Y302/01091 ,  Y02E50/16

Abstract: The present invention discloses a composition for biomass hydrolysis. The components of the composition may be obtained by biological or synthetic means. Synthetically, the components of the composition of the present invention may be obtained by amino acid synthesis or may be procured commercially.

公开(公告)号：US09896707B2

公开(公告)日：2018-02-20

申请号：US15191113

申请日：2016-06-23

Applicant: Battelle Energy Alliance, LLC

Inventor： David N. Thompson ,  William A. Apel ,  Vicki S. Thompson ,  David W. Reed ,  Jeffrey A. Lacey ,  Emily D. Henriksen

IPC: C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/88 ,  C12P19/14 ,  C12P19/04 ,  C07H21/04 ,  C07K1/00 ,  C12N9/10 ,  C12N9/24 ,  C12N9/26 ,  C12N9/42 ,  C12N9/40 ,  C12N9/38 ,  C12N15/52 ,  C12P19/00 ,  C12N9/34 ,  C12N9/80 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/80 ,  C12N9/88 ,  C12N15/52 ,  C12P19/00 ,  C12P19/02 ,  C12Y204/0102 ,  C12Y301/01001 ,  C12Y301/01006 ,  C12Y301/01073 ,  C12Y301/02004 ,  C12Y302/01008 ,  C12Y302/01015 ,  C12Y302/0102 ,  C12Y302/01021 ,  C12Y302/01022 ,  C12Y302/01037 ,  C12Y302/01055 ,  C12Y302/01078 ,  C12Y302/01091 ,  C12Y302/01131 ,  C12Y302/01133 ,  C12Y302/01139 ,  C12Y305/01 ,  C12Y402/01007 ,  C12Y402/01008

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.