知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

79 条记录 (耗时 0.017 秒)

    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding amino acids
    51.
    发明授权
    Process for the continuous enzymatic change of water soluble .alpha.-ketocarboxylic acids into the corresponding amino acids 失效
    将水溶性α-酮羧酸连续酶促转化为相应氨基酸的方法

    公开(公告)号：US4304858A

    公开(公告)日：1981-12-08

    申请号：US172446

    申请日：1980-07-25

    申请人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    发明人： Christian Wandrey ,  Rolf Wichmann ,  Wolfgang Leuchtenberger ,  Maria-Regina Kula ,  Andreas Buckmann

    IPC分类号： C07C229/06 ,  C12M1/34 ,  C12P1/00 ,  C12P13/04 ,  C12P13/06 ,  C12P19/36 ,  C12P13/08

    CPC分类号： C12P13/06 ,  C12P1/00 ,  C12P13/04 ,  C12P19/36 ,  Y10S435/813

    摘要： Water soluble .alpha.-ketocarboxylic acids are continuously converted in a membrane reactor into the corresponding aminoacids. The conversion takes place in the presence of a substrate specific dehydrogenase, of ammonium ions and of a nicotinamide-adenine-dinucleotide (NAD.sup.+ /NADH) enlarged in molecular weight through linkage to a water soluble polymer as coenzyme. Simultaneously NADH is regenerated continuously from NAD.sup.+ in presence of a formate dehydrogenase and from formate ion. The membrane must have a mean pore diameter of 1 to 3 nm. As coenzyme there is employed 0.1 to 10 mmol/l of NAD.sup.+ /NADH present bound to a polyoxyethylene having an average molecular weight between 500 and 50,000. There is continuously supplied to the reactor a substrate stream which contains 50 to 100% of the maximum amount soluble, but not over 2,000 mmol/l, of the reacting .alpha.-ketocarboxylic acid in the form of a water soluble salt, an ammonium ion in an amount about equimolar to the amount of substrate and 100 to 6,000 mmol/l of a formate. There is maintained over the membrane a differential pressure of 0.1 to 15 bar. There is continuously drawn off behind the membrane a filtrate stream containing the aminoacid formed.

    摘要翻译： 水溶性α-酮羧酸在膜反应器中连续转化成相应的氨基酸。 通过与作为辅酶的水溶性聚合物连接，在底物特异性脱氢酶，铵离子和烟酰胺 - 腺嘌呤二核苷酸（NAD + / NADH）存在下进行转化，分子量增加。 同时NADH在甲酸脱氢酶和甲酸根离子存在下从NAD +连续再生。 膜的平均孔径必须为1〜3nm。 作为辅酶，使用0.1〜10mmol / l的NAD + / NADH，其与平均分子量在500〜50,000的聚氧乙烯结合。 向反应器中连续供应底物流，其含有50〜100％最大量可溶性但不超过2,000mmol / l的水溶性盐，铵离子形式的α-酮羧酸 相当于底物量的等摩尔量和100-6,000mmol / l甲酸盐的量。 在膜上保持0.1至15巴的压差。 在膜的后面连续抽出含有形成的氨基酸的滤液流。

    Reactor for stabilized microbes having photometabolic activity
    52.
    发明授权
    Reactor for stabilized microbes having photometabolic activity 失效
    具有光代谢活性的稳定化微生物反应器

    公开(公告)号：US4010076A

    公开(公告)日：1977-03-01

    申请号：US672631

    申请日：1976-04-01

    申请人： Howard H. Weetall

    发明人： Howard H. Weetall

    IPC分类号： A01G33/00 ,  C12M1/00 ,  C12N1/12 ,  C12N11/04 ,  C12P1/04 ,  C12P3/00 ,  C12P19/36 ,  C12B1/00

    CPC分类号： C12P19/36 ,  A01G33/00 ,  C12M21/02 ,  C12M21/04 ,  C12M25/06 ,  C12M29/20 ,  C12N11/04 ,  C12P3/00 ,  Y10S423/17 ,  Y10S435/822 ,  Y10S435/946

    摘要： Useful products such as molecular hydrogen can be continuously produced by reacting a solution of a substrate in the presence of light with a photometabolically active microbe which has been stabilized on a support. In one embodiment, Rhodospirillium rubrum bacterial cells are mixed with agar gel and the mixture is spread over a plate which is placed in a transparent flow-through reactor. Then, an aqueous malate solution is passed through the reactor under essentially anaerobic conditions in the presence of light to yield molecular hydrogen. In another embodiment, Blue-Green algae are similarly stabilized for continuous biophotolysis of water by oxidizing the water and reducing NADP to NADPH. Other photosystems for producing useful products are disclosed.

    摘要翻译： 有用的产物如分子氢可以通过使光存在下的底物溶液与稳定在载体上的光代谢活性微生物反应来连续生产。 在一个实施方案中，红景天红细菌细菌细胞与琼脂凝胶混合，并将混合物分散在置于透明反应器中的板上。 然后，在光的存在下，在基本上无氧条件下，将苹果酸水溶液水溶液通过反应器，得到分子氢。 在另一个实施方案中，蓝绿藻类似地通过氧化水并将NADP还原成NADPH来稳定水的连续生物光解。 公开了用于生产有用产品的其他光系统。

    Process for preparing nicotinamide adenine dinucleotide phosphate
    53.
    发明授权
    Process for preparing nicotinamide adenine dinucleotide phosphate 失效
    制备尼古丁腺嘌呤磷酸酯的方法

    公开(公告)号：US3819481A

    公开(公告)日：1974-06-25

    申请号：US19041371

    申请日：1971-10-18

    申请人： KYOWA HAKKO KOGYO KK

    发明人： SHIMIZU Y ,  AKIYAMA Y ,  TATANO T

    IPC分类号： C12P19/36 ,  C12D13/06

    CPC分类号： C12P19/36 ,  Y10S435/83 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/859 ,  Y10S435/881 ,  Y10S435/921 ,  Y10S435/942

    摘要： A process for preparing nicotinamide adenine dinucleotide phosphate (NADP) by reacting nicotinamide adenine dinucleotide with adenosine triphosphate in the presence of nicotinamide adenine dinucleotide (NAD) kinase. The NAD kinase may be in the crude form and appreciable quantities of NADP are produced if the reaction takes place in the presence of fluoride and/or an organic sulf-hydral (SH) radical containing reagent. Good production also is achieved if the crude NAD kinase is treated with a mineral acid prior to the reaction. Exceptionally good yields are achieved if an acid-pretreated NAD kinase is used and both fluoride and an SH reagent are present during the reaction.

    摘要翻译： 在烟酰胺腺嘌呤二核苷酸（NAD）激酶存在下，烟酰胺腺嘌呤二核苷酸与三磷酸腺苷反应制备烟酰胺腺嘌呤二核苷酸磷酸（NADP）的方法。 NAD激酶可以是粗制形式，如果在氟化物和/或有机硫磺基（SH）基团的试剂的存在下发生反应，则产生可观量的NADP。 如果在反应之前用无机酸处理粗制NAD激酶，也可实现良好的生产。 如果使用酸预处理的NAD激酶并且在反应期间存在氟化物和SH试剂，则实现了非常好的产率。

    Method for producing nicotinamide adenine dinucleotide
    55.
    发明授权
    Method for producing nicotinamide adenine dinucleotide 失效
    生产尼古丁腺嘌呤核苷酸的方法

    公开(公告)号：US3705081A

    公开(公告)日：1972-12-05

    申请号：US3705081D

    申请日：1968-06-13

    申请人： KYOWA HAKKO KOGYO KK

    发明人： NAKAYAMA KIYOSHI

    IPC分类号： C12P19/36 ,  C12D13/06

    CPC分类号： C12P19/36 ,  Y10S435/83 ,  Y10S435/84 ,  Y10S435/843

    摘要： THE PRESENT DISCLOSURE RELATES TO A METHOD FOR PRODUCING NICOTINAMIDE AIDENINE DIMUCLEOTIDE IN HIGH YIELDS WHICH COMPRISES CULTURING A MICROORGANISM CAPABILE OF PRODUCING NICOTINAMIDE ADERINE DINUCLEOTIDE IN AN EQUEOUS NUTRIENT MEDIUM UNDER AEROBIC CONDITIONS IN THE PRESENCE OF PANTOTHENIC ACID, B-ALANINE OR COENZYME A AND MIXTURES THEREOF. EXAMPLES OF MICROORGANISMS WHICH CAN BE USED IN THE PRESENT PROCESS ARE THOSE BELONGING TO THE GENERA BREVIBACTERIUM, CORYNEBACTERIUM AND ARTHROBACTER.

    REGULATION OF POLYMERASE USING COFACTOR OXIDATION STATES
    60.
    发明公开
    REGULATION OF POLYMERASE USING COFACTOR OXIDATION STATES 审中-公开

    公开(公告)号：US20230348946A1

    公开(公告)日：2023-11-02

    申请号：US18221364

    申请日：2023-07-12

    申请人： MICROSOFT TECHNOLOGY LICENSING, LLC

    发明人： Bichlien Hoang NGUYEN

    IPC分类号： C12P19/36 ,  C12Q1/6874 ,  B01J19/00

    CPC分类号： C12P19/36 ,  B01J19/0046 ,  C12Q1/6874 ,  C12Y207/07031 ,  C12P19/34 ,  C12Y207/07006

    摘要： Polynucleotide synthesis performed with a substrate independent polymerase such as terminal deoxynucleotidyl transferase (TdT) is regulated by controlling the oxidation state of a metal cofactor. The oxidation state of the metal cofactor is changed to +2, thus activating the polymerase, by applying a voltage with electrodes or by introducing a chemical redox reagent. Addressable polynucleotide synthesis creates polynucleotides with different arbitrary sequences through use of spatial control of cofactor oxidation states to add nucleotides only at selected locations on an array. Control of metal oxidation states is regulated by selective activation of a microelectrode array, controlled addition of redox reagents to specific locations on the array, or controlled activation of photocatalysts at specific locations on the array. Scavengers in solution prevent cofactors distant from the selected locations from catalyzing polymerase activity and thereby maintain the localized effect of polymerase activation.