公开(公告)号：US20180344758A1

公开(公告)日：2018-12-06

申请号：US15533687

申请日：2015-12-15

申请人： LIPOGEN LLC

发明人： Pingwei Li ,  Chenguang Wang ,  Chang Shu

IPC分类号： A61K31/7084 ,  A61P35/00 ,  A61P35/02 ,  C12P19/36

CPC分类号： A61K31/7076 ,  C12P19/36

摘要： In one embodiment, a method of treating cancer in a patient comprises administering cGAMP or cGAsMP to a patient having cancer and allowing the cGAMP or cGAsMP to treat the cancer. In another embodiment, a method for en2ymatically synthesizing and purifying cGAMP or cGAsMP comprises providing cGAS; combining cGAS with ATP or ATP phosphorothioate, respectively, and GTP to produce cGAMP or cGAsMP; separating cGAMP or cGAsMP from the cGAS and DNA by ultrafiltration; and purifying cGAMP or cGAsMP using ion exchange chromatography and optionally gel filtration chromatography.

公开(公告)号：US20170218417A1

公开(公告)日：2017-08-03

申请号：US15490416

申请日：2017-04-18

申请人： ANNIKKI GMBH

发明人： Ortwin Ertl ,  Nicole Staunig ,  Marta Sut ,  Bernd Mayer

IPC分类号： C12P19/36 ,  C12P41/00 ,  C12P33/00

CPC分类号： C12P19/36 ,  C12P19/02 ,  C12P33/00 ,  C12P41/002

摘要： A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.

公开(公告)号：US09650615B2

公开(公告)日：2017-05-16

申请号：US14380873

申请日：2013-02-26

申请人： Yamasa Corporation

发明人： Kaori Tanabe ,  Kazuya Ishige

IPC分类号： C12N9/12 ,  C12P19/36

CPC分类号： C12N9/1241 ,  C07K2319/00 ,  C12N15/62 ,  C12P19/36 ,  C12Y207/07065

摘要： A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP→c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH7.8; and (F) the presence of GGDEF (SEQ ID NO:26) domain and the lack of amino acid sequence KXXD (SEQ ID NO:23) in the i-site.

公开(公告)号：US09644227B2

公开(公告)日：2017-05-09

申请号：US14376512

申请日：2013-02-06

申请人： ANNIKKI GMBH

发明人： Ortwin Ertl ,  Nicole Staunig ,  Marta Sut ,  Bernd Mayer

IPC分类号： C12P33/00 ,  C12P19/36 ,  C12P41/00 ,  C12P19/02

CPC分类号： C12P19/36 ,  C12P19/02 ,  C12P33/00 ,  C12P41/002

摘要： A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.

公开(公告)号：US20140256930A1

公开(公告)日：2014-09-11

申请号：US14129834

申请日：2012-06-27

申请人： Shinichi Yoshida ,  Shunichi Taira ,  Masakatsu Nishihachijyo ,  Masutoshi Nojiri ,  Shigeru Kawano

发明人： Shinichi Yoshida ,  Shunichi Taira ,  Masakatsu Nishihachijyo ,  Masutoshi Nojiri ,  Shigeru Kawano

IPC分类号： C12N9/04 ,  C07H19/207 ,  C12P19/36

CPC分类号： C12N9/0006 ,  C07H19/207 ,  C12P19/36 ,  Y02P20/52

摘要： An object of the present invention is to provide a method for converting the coenzyme dependency of enzymes of the medium-chain dehydrogenase/reductase (MDR) family. A further object of the present invention is to provide enzyme variants of the MDR family whose coenzyme dependency is converted by the conversion method and a method for enzymatically producing optically active alcohols using the enzymes. The present inventors developed a novel enzyme conversion method for converting the coenzyme dependency of enzymes of the MDR family, rationally designed enzyme variants that are altered by the enzyme conversion method to be able to use NADPH as a coenzyme from a useful enzyme of the MDR family that uses NADH as a coenzyme, and actually provide variants having such an ability.

摘要翻译： 本发明的目的是提供一种转化中链脱氢酶/还原酶（MDR）家族酶的辅酶依赖性的方法。 本发明的另一个目的是提供MDR家族的酶变体，其通过转化方法转化其辅酶依赖性，以及使用酶酶法产生光学活性醇的方法。 本发明人开发了一种用于转化MDR家族的酶的辅酶依赖性的新型酶转化方法，通过酶转化方法改变的合理设计的酶变体，以能够使用来自MDR家族的有用酶的NADPH作为辅酶 其使用NADH作为辅酶，实际上提供具有这种能力的变体。

公开(公告)号：US08709753B2

公开(公告)日：2014-04-29

申请号：US13680608

申请日：2012-11-19

申请人： William Marsh Rice University

发明人： Ka-Yiu San ,  George N. Bennett ,  Yipeng Wang

IPC分类号： C12N15/70

CPC分类号： C12N15/70 ,  C12N9/0008 ,  C12N9/1205 ,  C12P5/007 ,  C12P7/52 ,  C12P17/08 ,  C12P19/36 ,  C12Y101/01008 ,  C12Y101/01094 ,  C12Y207/01023

摘要： This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.

摘要翻译： 本发明是为提高NADPH依赖化合物的产量和生产力而提供增加的细胞内NADPH可用性的代谢工程菌株。 在本发明中，天然NAD依赖性GAPDH被NADP依赖性GAPDH加上过表达的NADK替代。 也提供了细菌的使用。

公开(公告)号：US07863014B2

公开(公告)日：2011-01-04

申请号：US10482791

申请日：2002-07-02

申请人： Shigeyuki Kawai ,  Kousaku Murata ,  Hirokazu Matsukawa ,  Shoichi Tomisako ,  Yoshio Ando ,  Yuhsi Matsuo

发明人： Shigeyuki Kawai ,  Kousaku Murata ,  Hirokazu Matsukawa ,  Shoichi Tomisako ,  Yoshio Ando ,  Yuhsi Matsuo

IPC分类号： C12P1/00

CPC分类号： C12P19/36

摘要： The present invention provides a novel process for preparing nicotinamide adenine dinucleotide phosphate (NADP). The process of the present invention comprises performing phosphorylation using a polyphosphoric acid or a salt thereof and nicotinamide adenine dinucleotide (NAD+) as substrates in the presence of a polyphosphate-dependent NAD+ kinase from a Mycobacterium, wherein the reaction solution contains 0.1-15% by weight of the polyphosphoric acid or a salt thereof, and 5-150 mM of a divalent metal ion.

摘要翻译： 本发明提供了制备烟酰胺腺嘌呤二核苷酸磷酸（NADP）的新方法。 本发明的方法包括在来自分枝杆菌的多磷酸依赖性NAD +激酶存在下，使用多磷酸或其盐和烟酰胺腺嘌呤二核苷酸（NAD +）作为底物进行磷酸化，其中反应溶液含有0.1-15％ 多磷酸或其盐的重量和5-150mM的二价金属离子。

公开(公告)号：US20090246803A1

公开(公告)日：2009-10-01

申请号：US11575605

申请日：2005-09-20

申请人： Shin-Ichiro Imai ,  Javier R. Revollo ,  Andrew A. Grimm

发明人： Shin-Ichiro Imai ,  Javier R. Revollo ,  Andrew A. Grimm

IPC分类号： C12P19/36 ,  G01N33/573 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12Q1/02

CPC分类号： C12P19/36 ,  C12N9/1077

摘要： The present invention generally relates to a nicotinamide adenine dinucleotide (NAD) biosynthesis system and methods of screening for NAD biosynthesis effectors. Among the various aspects of the present invention is the provision of an in vitro-reconstituted mammalian NAD biosynthesis system that can be used for the high-throughput screening of chemical activators and inhibitors for mammalian NAD biosynthesis. Another aspect of the invention provides a method of identifying a compound that effects in vivo activity of NAD metabolic enzymes. Further aspects of the invention include nucleic acid sequences, vectors, and transformed cells that can be used in the methods described herein.

摘要翻译： 本发明一般涉及烟酰胺腺嘌呤二核苷酸（NAD）生物合成系统和筛选NAD生物合成效应物的方法。 在本发明的各个方面中，提供体外重构的哺乳动物NAD生物合成系统，其可用于化学活化剂的高通量筛选和用于哺乳动物NAD生物合成的抑制剂。 本发明的另一方面提供了鉴定影响NAD代谢酶的体内活性的化合物的方法。 本发明的其它方面包括可用于本文所述方法的核酸序列，载体和转化的细胞。

公开(公告)号：US07195889B2

公开(公告)日：2007-03-27

申请号：US10308778

申请日：2002-12-03

申请人： Robert T. Hembre ,  Paul S. Wagenknecht ,  Jonathan M. Penney

发明人： Robert T. Hembre ,  Paul S. Wagenknecht ,  Jonathan M. Penney

IPC分类号： C12Q1/26

CPC分类号： C12P21/00 ,  C12N9/0004 ,  C12P1/00 ,  C12P7/02 ,  C12P19/36

摘要： The invention relates to processes for stabilizing the activity of an enzyme, comprising mixing a phosphine or phosphite with an oxidoreductase enzyme.

摘要翻译： 本发明涉及稳定酶的活性的方法，包括将膦或亚磷酸酯与氧化还原酶混合。

公开(公告)号：US20030157677A1

公开(公告)日：2003-08-21

申请号：US09996008

申请日：2001-11-28

发明人： Kazuya Mitsuhashi ,  Hiroaki Yamamoto ,  Norihiro Kimoto

IPC分类号： C12P019/36 ,  C12P007/62 ,  C12N009/02 ,  C07H021/04 ,  C12N001/18 ,  C12N015/74

CPC分类号： C12N9/0008 ,  C12P7/02 ,  C12P19/36

摘要： An objective of the present invention is to provide polypeptides capable of retaining a strong enzyme activity of formate dehydrogenase in the presence of an organic solvent and to provide the uses thereof. Formate dehydrogenase mutant polypeptides, which are resistant to organic solvents, were constructed by substituting cysteines at position 146 and/or at position 256 in the amino acid sequence of Mycobacterium vaccae-derived formate dehydrogenase by site-directed mutagenesis. The polypeptides have strong activities of formate dehydrogenase in the presence of an organic solvent. The mutants are useful for the production of alcohols using ketones as raw material, etc.

摘要翻译： 本发明的目的是提供能够在有机溶剂存在下保持甲酸脱氢酶的强酶活性并提供其用途的多肽。 通过定点诱变将来自疫苗分枝杆菌来源的甲酸脱氢酶的氨基酸序列中的146位和/或256位的半胱氨酸取代为对有机溶剂有抗性的甲酸脱氢酶突变体多肽。 多肽在有机溶剂存在下具有强的甲酸脱氢酶活性。 该突变体可用于以酮为原料生产醇类等。