公开(公告)号：US07335470B2

公开(公告)日：2008-02-26

申请号：US10273399

申请日：2002-10-15

Applicant: Mansoor Mohammed ,  Jason Kang ,  Shishir Shah ,  Wei-Wen Cai

Inventor： Mansoor Mohammed ,  Jason Kang ,  Shishir Shah ,  Wei-Wen Cai

IPC: C12Q1/68 ,  C12N15/00 ,  C12N15/11

CPC classification number: C40B40/08 ,  C07H21/00 ,  C40B20/04 ,  C40B30/04 ,  C40B60/10

Abstract: In one aspect the invention provides compilations of nucleic acids, articles of manufacture, e.g., arrays, and methods for the detection of chromosomal abnormalities, such as a chromosomal aneuploidies, deletions, amplifications, and the like, and the diagnosis or prognosis of syndromes associated with a contiguous gene abnormality. Kits are also provided.

Abstract translation: 在一个方面，本发明提供了核酸，制品，例如阵列和用于检测染色体异常（例如染色体非整倍体，缺失，扩增等）的方法的汇编，以及相关的综合征的诊断或预后 具有连续的基因异常。 还提供套件。

公开(公告)号：US07041510B2

公开(公告)日：2006-05-09

申请号：US09768414

申请日：2001-01-24

Applicant: Michael Seul ,  Chiu Wo Chau

Inventor： Michael Seul ,  Chiu Wo Chau

IPC: G01N33/543

CPC classification number: H05H3/04 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0454 ,  B01L2400/086 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B60/10 ,  G01N15/0266 ,  G01N30/0005 ,  G01N33/54313 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2030/0035 ,  G02B3/0012 ,  G02B3/0056 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (“gray scales”) of illumination patterns. The latter capability permits the creation of patterns with abruptly changing light intensities or patterns with gradually changing intensity profiles.

公开(公告)号：US20050059041A1

公开(公告)日：2005-03-17

申请号：US10847149

申请日：2004-05-17

Applicant: Robert Johnson ,  Mansoor Mohammed ,  Jae Kim ,  Xan-Yan Lu

Inventor： Robert Johnson ,  Mansoor Mohammed ,  Jae Kim ,  Xan-Yan Lu

IPC: C07H21/00 ,  C12Q1/68 ,  C12M1/34

CPC classification number: C07H21/00 ,  C40B20/04 ,  C40B30/04 ,  C40B40/08 ,  C40B60/10

Abstract: Compilations of nucleic acids, articles of manufacture which are surfaces comprising multiple blocks of arrays comprising such compilations, methods of use of the compilations and arrays for detection of chromosomal disorders, such as a chromosomal aneuploidies, deletions, amplifications, and diagnosis and prognosis of syndromes associated with a contiguous gene abnormality and kits are provided.

Abstract translation: 核酸的制备，制品是包含多个阵列阵列的表面，包括该组合物，使用该组合物的方法和用于检测染色体病症的染色体异常，例如染色体非整倍体，缺失，扩增以及诊断和预后的综合征 提供与连续基因异常相关的试剂盒。

公开(公告)号：US20040136875A1

公开(公告)日：2004-07-15

申请号：US10656535

申请日：2003-09-05

Inventor： Michael Seul ,  Chiu Wo Chau

IPC: B01L003/00

CPC classification number: H05H3/04 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0454 ,  B01L2400/086 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B60/10 ,  G01N15/0266 ,  G01N30/0005 ,  G01N33/54313 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2030/0035 ,  G02B3/0012 ,  G02B3/0056 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (nulltime-varying patternsnull), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (nullgray scalesnull) of illumination patterns. The latter capability permits the creation of patterns with abruptly changing light intensities or patterns with gradually changing intensity profiles.

公开(公告)号：US20030124028A1

公开(公告)日：2003-07-03

申请号：US10156222

申请日：2002-05-24

Inventor： Eric D. Carlson ,  Peijun Cong ,  William H. Chandler JR. ,  Henry K. Chau ,  Earl Danielson ,  Peter J. Desrosiers ,  Robert D. Doolen ,  Luping Wu ,  C. Eric Ramberg ,  Thomas Crevier ,  Ralph B. Nielsen ,  Colin S. Masui ,  John F. Varni

IPC: G01N021/47 ,  G01N033/00

CPC classification number: B01L3/50255 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00308 ,  B01J2219/00315 ,  B01J2219/00335 ,  B01J2219/00364 ,  B01J2219/00373 ,  B01J2219/00423 ,  B01J2219/00481 ,  B01J2219/00484 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00725 ,  B01J2219/00756 ,  B01L3/06 ,  B01L7/00 ,  B01L2300/0829 ,  B01L2300/185 ,  C40B40/04 ,  C40B40/10 ,  C40B60/10 ,  C40B60/14 ,  G01N5/045 ,  G01N21/23 ,  G01N21/65 ,  G01N25/04 ,  G01N33/15 ,  G01N33/54366 ,  G01N35/028 ,  G01N2013/006 ,  G01N2021/651 ,  G01N2500/00 ,  G16C20/20 ,  G16C20/90 ,  Y10T117/10

Abstract: The invention provides methods, apparatus, and systems for performing high-throughput preparation and screening of salts and polymorphs of drug candidates. The invention is directed towards enhancing the pre-formulation discovery process used for drug development. In particular, processes that determine suitable salts and processes that discover substantially every polymorph that can form from a particular drug candidate are provided. The processes are performed using several apparatuses that are specifically configured to carry-out various steps in a high-throughput characterization process. One such apparatus is configured for synthesizing a plurality of library members based on, for example, a library model generated by a computer system. Another apparatus may filter the synthesized solution to provide a substantially pure mixture that can be subjected to salt or polymorph testing. Yet another apparatus may be used to crystallize mixtures on a substrate such that the crystallized mixture can be screened by one or more screening devices.

Abstract translation: 本发明提供了用于进行候选药物的盐和多晶型物的高通量制备和筛选的方法，装置和系统。 本发明旨在增强用于药物开发的预配制发现过程。 特别地，提供了确定基本上发现可从特定药物候选物形成的每个多晶型物的合适的盐和方法的方法。 使用特定配置为在高吞吐量表征过程中执行各种步骤的几个装置来执行处理。 一种这样的装置被配置为基于例如由计算机系统生成的库模型来合成多个库成员。 另一种装置可以过滤合成的溶液以提供可以进行盐或多晶型测试的基本上纯的混合物。 可以使用另一种装置将混合物在基底上结晶，使得结晶的混合物可以被一个或多个筛选装置筛选。

公开(公告)号：US20160108469A9

公开(公告)日：2016-04-21

申请号：US13481858

申请日：2012-05-27

Applicant: Hesaam Esfandyarpour ,  Kosar B. Parizi ,  Mark F. Oldham

Inventor： Hesaam Esfandyarpour ,  Kosar B. Parizi ,  Mark F. Oldham

IPC: C12Q1/68 ,  G01N27/414 ,  C12M1/34

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.

公开(公告)号：US20150368707A1

公开(公告)日：2015-12-24

申请号：US14835070

申请日：2015-08-25

Applicant: GenapSys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark Oldham

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US20150346188A1

公开(公告)日：2015-12-03

申请号：US14653963

申请日：2013-12-20

Applicant: IBIS BIOSCIENCES, INC.

Inventor： Mark W. Eshoo

IPC: G01N33/487

CPC classification number: G01N33/48721 ,  B82Y15/00 ,  B82Y30/00 ,  C40B60/10

Abstract: The invention relates generally to nanopore and micropore structures, methods for making the structures and use of the structures. In particular, the invention provides nanopore structures (e.g., containing lipid bilayers) in combination with one or more osmoprotective compounds and methods of making and using the same. Compositions and methods of the invention fmd use in a wide range of applications including molecular biology and medical science.

Abstract translation: 本发明一般涉及纳米孔和微孔结构，制造结构的方法和结构的使用。 特别地，本发明提供与一种或多种渗透保护性化合物组合的纳米孔结构（例如含有脂双层）及其制备和使用方法。 本发明的组合物和方法可用于广泛的应用，包括分子生物学和医学科学。

公开(公告)号：US09187783B2

公开(公告)日：2015-11-17

申请号：US13824129

申请日：2011-10-04

Applicant: Hesaam Esfandyarpour ,  Mark F. Oldham ,  Eric S. Nordman ,  Kosar Baghbani Parizi

Inventor： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Eric S. Nordman ,  Kosar Baghbani Parizi

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

Abstract translation: 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US20150148264A1

公开(公告)日：2015-05-28

申请号：US14596111

申请日：2015-01-13

Applicant: Genapsys, Inc.

Inventor： Hesaam Esfandyarpour ,  Mark Oldham

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.