公开(公告)号：US11891594B2

公开(公告)日：2024-02-06

申请号：US17514426

申请日：2021-10-29

Applicant: Fluid-Screen, Inc.

Inventor： Monika Weber ,  Slawomir Antoszczyk ,  Robert Weber

IPC: B01L3/00 ,  C12M3/06 ,  G01N27/447 ,  G01N33/569 ,  C12Q1/04 ,  C12Q1/24 ,  G01N21/64 ,  B03C5/00 ,  C12Q1/06

CPC classification number: C12M23/16 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B03C5/005 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/24 ,  G01N21/6428 ,  G01N21/6458 ,  G01N27/44791 ,  G01N33/56911 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0475 ,  B01L2400/082 ,  G01N2021/6439 ,  G01N2201/062

Abstract: Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.

公开(公告)号：US11883818B2

公开(公告)日：2024-01-30

申请号：US17834410

申请日：2022-06-07

Applicant: bioMerieux, Inc. ,  BioFire Defense, LLC

Inventor： Kirk Ririe ,  Christopher S. Ronsick ,  Mark S. Wilson ,  John D. Walsh ,  Ryan T. Hill

IPC: B01L1/00 ,  B01L3/00 ,  G01N1/40 ,  C12M1/00 ,  B04B5/04 ,  C12Q1/24 ,  B01L3/02 ,  G01N1/20 ,  B04B11/04

CPC classification number: B01L3/50215 ,  B01L3/0296 ,  B01L3/5021 ,  B01L3/5082 ,  B01L3/50825 ,  B04B5/0414 ,  C12M45/05 ,  C12Q1/24 ,  G01N1/2035 ,  G01N1/4077 ,  B01L2200/02 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/0848 ,  B01L2300/123 ,  B01L2400/0409 ,  B01L2400/0478 ,  B01L2400/0633 ,  B01L2400/0683 ,  B04B2005/0435 ,  B04B2011/046 ,  G01N2001/4083

Abstract: A separation container for extracting a portion of a sample for use or testing and method for preparing samples for downstream use or testing are provided. The separation container may include a body defining an internal chamber. The body may define an opening, and the body may be configured to receive the sample within the internal chamber. The separation container may further include a seal disposed across the opening, such that the seal may be configured to seal the opening of the body, and a plunger movably disposed at least partially inside the internal chamber. The plunger may be configured to be actuated to open the seal and express the portion of the sample.

公开(公告)号：US11865539B2

公开(公告)日：2024-01-09

申请号：US17704102

申请日：2022-03-25

Applicant: MURSLA LIMITED

Inventor： Tomas Miguel De Freitas Dias ,  Pierre Arsène

IPC: G01N27/32 ,  B01L3/00 ,  G01N27/327 ,  G01N33/543 ,  B03C5/00 ,  G01N27/447

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  B03C5/005 ,  G01N27/3274 ,  G01N27/3276 ,  G01N27/3278 ,  G01N27/44704 ,  G01N33/5438 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54386 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B03C2201/26

Abstract: A method of detecting a target biological entity in a biofluid using a sensor, wherein the biofluid comprises a plurality of the target biological entities and nanoparticles, the sensor comprising a substrate bearing a pair of electrodes having an affinity with the nanoparticles, and wherein a region between the electrodes defines a sensing region. The method comprises: treating the biofluid with a suspension comprising a plurality of nanoparticles to obtain a treated mixture comprising bound nanoparticle-entity assemblies; introducing the treated mixture to the sensor; conditioning the sensor in the presence of the treated mixture by applying an activation voltage between the electrodes to increase a degree of connection between a surface of the pair of electrodes and at least one bound nanoparticle-entity assembly in contact with the surface of the pair of electrodes; and detecting the presence of target biological entities by using the pair of electrodes to detect a current through the at least one bound nanoparticle-entity assembly.

公开(公告)号：US11857967B2

公开(公告)日：2024-01-02

申请号：US16339561

申请日：2017-10-06

Applicant: University of Maryland, College Park

Inventor： Donald Lad DeVoe ,  Jung Yeon Han

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/08 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/12 ,  B01L2400/0406 ,  B01L2400/086

Abstract: In an embodiment, a microfluidic chip includes a capillary is disposed between upper and lower substrates, where the capillary includes a porous monolithic structure disposed within the capillary, and a clamp structure is defined within the channel and engages with the capillary. The clamp structure comprises a thermoplastic material that, when heated to a selected temperature, deforms around the capillary to secure the capillary in alignment with the channel. In another embodiment, a microfluidic chip includes a porous monolithic brick disposed between first and second substrates, where each of the first and second substrates includes a channel extending through the substrate to the brick structure to provide a fluid flow path through the each of the first substrate, the second substrate and the brick structure.

公开(公告)号：US20230398538A1

公开(公告)日：2023-12-14

申请号：US18199744

申请日：2023-05-19

Applicant: CYTENA GmbH

Inventor： Jonas FÖLLING

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2300/0654 ,  B01L2300/168 ,  B01L2300/0681 ,  B01L2200/0652

Abstract: The invention relates to a device having a dispenser for receiving liquid sample which has liquid and particles, a first light source of a first type for emitting a first illumination light for illuminating a first region of the dispenser, a first detection device for detecting a first optical measurement signal that emanates from the first region of the dispenser illuminated with the first illuminating light, a second light source of a second type for emitting a second illuminating light for illuminating a second region of the dispenser which comprises the first region of the dispenser, and a second detection device for detecting a second optical measurement signal which emanates from the second region of the dispenser illuminated with the second illuminating light. The device is characterized in that the first detection device is a point detector.

公开(公告)号：US20230358734A1

公开(公告)日：2023-11-09

申请号：US18143726

申请日：2023-05-05

Applicant: The Trustees of Princeton University

Inventor： Alexei Goun ,  Herschel Rabitz

IPC: G01N33/543 ,  B01L3/00

CPC classification number: G01N33/54373 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2200/0652

Abstract: Disclosed is an enabling process for high throughput manufacturing of large variety of chemical and biological sensors that utilize well-developed spin-based sensing of analyte binding. A method for producing a nanometer scale analyte specific sensor may be provided, that includes providing a first material (such as a diamond) having a luminescent center (such as an NV center) within predetermined distance (such as 2-5 nm) of a first surface, where the luminescent center configured to emit within an optical emission range, providing photochemically active molecules (such as molecules with a photo-uncaging protective group) where an optical absorption range of the photochemically active molecules at least partially overlaps the optical emission range, coating the first surface of the first material with the photochemically active molecules, and may include placement of the photochemically active surface molecule within a distance smaller than the Forster Resonant Excitation Transfer (i.e., 2-10 nm) from a luminescent center.

公开(公告)号：US20230358665A1

公开(公告)日：2023-11-09

申请号：US18214230

申请日：2023-06-26

Applicant: NODEXUS INC.

Inventor： Hany Nassef ,  Karthik Balakrishnan ,  Anand Kesavaraju ,  Vincent Tuminelli ,  George Anwar

IPC: G01N15/14 ,  B07C5/342 ,  B01L3/00 ,  B07C5/344 ,  G01N15/10

CPC classification number: G01N15/1484 ,  B07C5/3425 ,  B01L3/502715 ,  G01N15/1459 ,  B07C5/344 ,  B01L3/502761 ,  B01L2400/06 ,  G01N2015/1081 ,  B01L2300/0874 ,  B01L2300/0627 ,  G01N2015/1006 ,  B01L2200/0652 ,  B01L2300/0816

Abstract: Disclosed herein is a system to detect and characterize individual particles and cells using at least either optic or electric detection as the particle or cell flows through a microfluidic channel. The system also provides for sorting particles and cells or isolating individual particles and cells.

公开(公告)号：US20230356225A1

公开(公告)日：2023-11-09

申请号：US18246243

申请日：2021-09-27

Applicant: Georgia Tech Research Corporation

Inventor： Emily L. Jackson-Holmes ,  Gongchen Sun ,  Guillaume A. Aubry ,  Hang Lu

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0883 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/086

Abstract: Microfluidic devices for capturing particles, such as cells, are disclosed. An example device includes a main fluid channel, a first interaction chamber in fluid communication with the main fluid channel, a first particle trap extending from a first wall of the main fluid channel and positioned between the main fluid channel and the first interaction chamber, and a second particle trap extending from a second wall of the main fluid channel and positioned between the main fluid channel and the first interaction chamber. The first particle trap and the second particle trap are in fluid communication both via the first interaction chamber and via the main fluid channel. Methods of using the devices are also disclosed.

公开(公告)号：US11808767B2

公开(公告)日：2023-11-07

申请号：US17932687

申请日：2022-09-16

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Mengxia Zhao ,  Wyatt Nelson ,  Perry G. Schiro

IPC: G01N33/574 ,  B01L3/00 ,  G01N15/06 ,  G01N15/14 ,  G01N33/49 ,  G01N21/64 ,  G01N33/53 ,  G01N35/10 ,  G01N35/00 ,  G01N15/10

CPC classification number: G01N33/57492 ,  B01L3/502761 ,  B01L3/561 ,  B01L3/567 ,  G01N15/0612 ,  G01N15/0656 ,  G01N15/1456 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/49 ,  G01N33/5304 ,  B01L3/502738 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/06 ,  B01L2400/0633 ,  B01L2400/086 ,  G01N15/0618 ,  G01N35/0098 ,  G01N2015/0681 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00237 ,  G01N2035/00356 ,  G01N2035/1034

Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

公开(公告)号：US20230347347A1

公开(公告)日：2023-11-02

申请号：US18068207

申请日：2022-12-19

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2300/087 ,  B01L2300/0816 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2200/0652 ,  G01N2469/20

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.