公开(公告)号：US20210156819A1

公开(公告)日：2021-05-27

申请号：US16626011

申请日：2019-02-13

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jens Gundlach

IPC: G01N27/414 ,  H01L29/786 ,  H01L29/66 ,  H01L21/74 ,  G01N33/487

Abstract: Example devices include a cis well associated with a cis electrode, a trans well associated with a trans electrode, and a field effect transistor (FET) positioned between the cis well and the trans well. Examples of the field effect transistor (FET) include a fluidic system defined therein. The fluidic system includes a first cavity facing the cis well, a second cavity fluidically connected to the trans well, and a through via extending through the field effect transistor from the first cavity. A first nanoscale opening fluidically connects the cis well and the first cavity, the first nanoscale opening having an inner diameter. A second nanoscale opening fluidically connects the through via and the second cavity, the second nanoscale opening having an inner diameter. The second nanoscale opening inner diameter is larger than the first nanoscale opening inner diameter.

公开(公告)号：US20210139975A1

公开(公告)日：2021-05-13

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US10960377B2

公开(公告)日：2021-03-30

申请号：US16433346

申请日：2019-06-06

Applicant: ILLUMINA, INC.

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Boyan Boyanov

IPC: C12P19/34 ,  B01J19/00 ,  C12Q1/6837 ,  C12Q1/68

Abstract: Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

公开(公告)号：US20200318167A1

公开(公告)日：2020-10-08

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US10545115B2

公开(公告)日：2020-01-28

申请号：US15839795

申请日：2017-12-12

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Jeffrey G. Mandell ,  Jingwei Bai ,  Kevin L. Gunderson ,  Cheng-Yao Chen ,  Michel Perbost

IPC: C12N9/00 ,  C12N9/96 ,  G01N27/414 ,  C12Q1/6869

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US10519499B2

公开(公告)日：2019-12-31

申请号：US15522987

申请日：2015-07-29

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US20190204225A1

公开(公告)日：2019-07-04

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N21/645 ,  B01L3/502707 ,  B01L3/502715 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/54373 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US10254225B2

公开(公告)日：2019-04-09

申请号：US15175489

申请日：2014-12-09

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/05 ,  G01N21/64 ,  B01L3/00 ,  G01N33/543

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. The biosensor also includes a shield layer having apertures that are positioned relative to the input regions of corresponding light guides such that the light emissions propagate through the apertures into the corresponding input regions. The shield layer extends between adjacent apertures and is configured to block the excitation light and the light emissions incident on the shield layer between the adjacent apertures.

公开(公告)号：US20190041354A1

公开(公告)日：2019-02-07

申请号：US16024299

申请日：2018-06-29

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov

IPC: G01N27/414 ,  H03K3/03 ,  C12Q1/6869 ,  H01L21/02

CPC classification number: G01N27/4145 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6869 ,  G01N27/4146 ,  G01R19/0092 ,  H01L21/0259 ,  H01L29/068 ,  H01L29/775 ,  H03K3/0315 ,  C12Q2563/157 ,  C12Q2565/607

Abstract: Apparatus and methods are disclosed for single molecule field effect sensors having conductive channels functionalized with a single active moiety. A region of a nanostructure (e.g., such as a silicon nanowire or a carbon nanotube) provide the conductive channel. Trapped state density of the nanostructure is modified for a portion of the nanostructure in proximity with a location where the active moiety is linked to the nanostructure. In one example, the semiconductor device includes a source, a drain, a channel including a nanostructure having a modified portion with an increased trap state density, the modified portion being further functionalized with an active moiety. A gate terminal is in electrical communication with the nanostructure. As a varying electrical signal is applied to an ionic solution in contact with the nanostructure channel, changes in current observed from the semiconductor device can be used to identify composition of the analyte.

公开(公告)号：US20170356030A1

公开(公告)日：2017-12-14

申请号：US15507465

申请日：2015-09-10

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril De Lattre ,  Tarun Khurana

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.