公开(公告)号：US10961576B2

公开(公告)日：2021-03-30

申请号：US16707554

申请日：2019-12-09

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N33/487 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US10350570B2

公开(公告)日：2019-07-16

申请号：US15535695

申请日：2015-12-14

Applicant: Illumina, Inc.

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Boyan Boyanov

IPC: C12Q1/68 ,  B01J19/00 ,  C12Q1/6837

Abstract: Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

公开(公告)号：US11732301B2

公开(公告)日：2023-08-22

申请号：US17188193

申请日：2021-03-01

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N33/487 ,  G01N27/447

CPC classification number: C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US20180141020A1

公开(公告)日：2018-05-24

申请号：US15535695

申请日：2015-12-14

Applicant: Illumina, Inc.

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Boyan Boyanov

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00274 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q2565/543 ,  C12Q2521/543

Abstract: Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

公开(公告)号：US10605766B2

公开(公告)日：2020-03-31

申请号：US14798762

申请日：2015-07-14

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Jeffrey G. Mandell ,  Jingwei Bai ,  Kevin L. Gunderson ,  Cheng-Yao Chen ,  Michel Perbost

IPC: C12Q1/68 ,  G01N27/414 ,  C12Q1/6869 ,  C12Q1/6825 ,  B82Y15/00

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US10787704B2

公开(公告)日：2020-09-29

申请号：US15572741

申请日：2016-05-11

Applicant: Illumina, Inc. ,  The Regents of the University of California

Inventor： Kevin L Gunderson ,  Jingwei Bai ,  Cheng-Yao Chen ,  Jeffrey G Mandell ,  Sergio Peisajovich ,  Philip G Collins ,  Gregory A Weiss ,  Boyan Boyanov

IPC: C12P19/34 ,  C12Q1/6869

Abstract: The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.

公开(公告)号：US20180155773A1

公开(公告)日：2018-06-07

申请号：US15572741

申请日：2016-05-11

Applicant: Illumina, Inc. ,  The Regents of the University of California

Inventor： Kevin L Gunderson ,  Jingwei Bai ,  Cheng-Yao Chen ,  Jeffrey G Mandell ,  Sergio Peisajovich ,  Philip G Collins ,  Gregory A Weiss ,  Boyan Boyanov

IPC: C12Q1/6869

Abstract: The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.

公开(公告)号：US10960377B2

公开(公告)日：2021-03-30

申请号：US16433346

申请日：2019-06-06

Applicant: ILLUMINA, INC.

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Boyan Boyanov

IPC: C12P19/34 ,  B01J19/00 ,  C12Q1/6837 ,  C12Q1/68

Abstract: Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

公开(公告)号：US10545115B2

公开(公告)日：2020-01-28

申请号：US15839795

申请日：2017-12-12

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Jeffrey G. Mandell ,  Jingwei Bai ,  Kevin L. Gunderson ,  Cheng-Yao Chen ,  Michel Perbost

IPC: C12N9/00 ,  C12N9/96 ,  G01N27/414 ,  C12Q1/6869

Abstract: A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

公开(公告)号：US10519499B2

公开(公告)日：2019-12-31

申请号：US15522987

申请日：2015-07-29

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.