公开(公告)号：US11597937B2

公开(公告)日：2023-03-07

申请号：US15634803

申请日：2017-06-27

Applicant: Icon Genetics GmbH

Inventor： Ernst Weber ,  Stefan Werner ,  Carola Engler ,  Ramona Grutzner ,  Sylvestre Marillonnet

IPC: C40B50/06 ,  C12N15/66 ,  C12N15/10 ,  C40B40/10

Abstract: System for producing a nucleic acid construct of interest, said system comprising: a set of n entry DNAs numbered 1 to n, n being an integer of at least 2, each of said n entry DNAs comprising in this order: (i) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof; (ii) a sequence portion linking the cleavage site of said recognition site of item (i) with the cleavage site of the recognition site of the following item (iii), and (iii) a cleavage site of a further type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site; the cleavage sites of the type IIs restriction endonuclease recognition sites of item (iii) of entry DNAs 1 to n−1 are complementary to the cleavage sites of the type IIs restriction endonuclease recognition sites of item (i) of entry DNAs 2 to n, respectively; the cleavage site of the type IIs restriction endonuclease recognition site of item (iii) of entry DNA n is complementary to the cleavage site of the type IIs restriction endonuclease recognition site of item (i) of entry DNA 1 for allowing annealing of complementary single-stranded overhangs formed by restriction at recognition site (i) of entry DNA 1 and at recognition site (iii) of entry DNA n; said system further comprising a destination vector comprising in this order: (I) a type IIs restriction endonuclease recognition site followed by the cleavage site thereof; (II) a vector backbone preferably comprising a selectable marker gene, said vector backbone linking the cleavage sites of said recognition sites of items (I) and the following item (III); (III) a further cleavage site of a type IIs restriction endonuclease recognition site followed by the recognition site of said cleavage site, and (IV) optionally, an insert between the recognition sites of item (III) and item (I); said cleavage sites of items (I) and (III) being different and non-complementary, said recognition sites of items (I) and (III) being preferably recognitions sites of the same endonuclease.

公开(公告)号：US11578427B2

公开(公告)日：2023-02-14

申请号：US17400471

申请日：2021-08-12

Applicant: MOLECULAR PARTNERS AG

Inventor： Hans Kaspar Binz ,  Johannes Schilling ,  Patrik Forrer ,  Victor Levitsky ,  Natalia Venetz ,  Marcel Walser

IPC: C07K14/47 ,  C40B40/10 ,  A61K38/00

Abstract: The present invention relates to designed ankyrin repeat domains with altered surface residues, as well as to proteins comprising such a designed ankyrin repeat domain, nucleic acids encoding such domains or proteins, methods of preparing such proteins, pharmaceutical compositions comprising such proteins or nucleic acids, and the use of such proteins, nucleic acids or pharmaceutical compositions in the treatment of diseases.

公开(公告)号：US11499249B2

公开(公告)日：2022-11-15

申请号：US16692220

申请日：2019-11-22

Applicant: F-star Therapeutics Limited

Inventor： Gottfried Himmler ,  Florian Ruker ,  Gordana Wozniak-Knopp

IPC: C40B40/08 ,  C07K19/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/40 ,  C40B40/10

Abstract: Libraries of immunoglobulins which each have one or more amino acid modifications in at least one structural loop region of such immunoglobulins, where the modified loop region specifically binds to an epitope of an antigen to which an unmodified immunoglobulin does not significantly bind.

公开(公告)号：US11434585B2

公开(公告)日：2022-09-06

申请号：US16752140

申请日：2020-01-24

Applicant: INSTITUT PASTEUR ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： Frédéric Pecorari ,  Pedro Alzari

IPC: C40B40/10 ,  C07K14/195 ,  C07K16/12 ,  C07K16/42 ,  C12N15/10 ,  C40B30/04

Abstract: The present invention pertains to the field of protein engineering, and provides means for obtaining stable molecules that specifically bind to a target selected amongst a large variety of ligands families. In particular, the present invention provides methods for obtaining a molecule specifically binding to a target of interest, through a combinatorial mutation/selection approach with an OB-fold protein as a starting molecule. In particular, the target of interest can be of a different chemical nature form that of the native target of the OB-fold protein used as the starting molecule.

公开(公告)号：US20220162773A1

公开(公告)日：2022-05-26

申请号：US17531618

申请日：2021-11-19

Applicant: Becton, Dickinson and Company

Inventor： Cynthia Sakofsky ,  Katherine Lazaruk ,  Margaret Nakamoto ,  Devon Joseph Jensen

IPC: C40B40/10 ,  C12N15/10 ,  C40B50/06

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining the expression of highly expressed proteins and lowly expressed proteins. In some embodiments, primers allowing generation of separate libraries for abundant AbSeq protein profiling oligonucleotides and scarce AbSeq protein profiling oligonucleotides are provided.

公开(公告)号：US20220106708A1

公开(公告)日：2022-04-07

申请号：US17554373

申请日：2021-12-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N15/10 ,  C12N15/85 ,  C40B30/04 ,  C07K14/74 ,  C07K16/28 ,  C12N15/81 ,  C40B20/04 ,  C40B70/00 ,  C12N9/22 ,  C40B40/02 ,  C40B40/10 ,  C12N15/90

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210403900A1

公开(公告)日：2021-12-30

申请号：US16911398

申请日：2020-06-25

Applicant: Philip David Rodley

Inventor： Philip David Rodley

IPC: C12N15/10 ,  C40B40/10

Abstract: The invention provides a protein scaffold and methods of preparing, screening, engineering and using the protein scaffold.

公开(公告)号：US11124556B2

公开(公告)日：2021-09-21

申请号：US15759775

申请日：2016-09-15

Applicant: ADAPTIMMUNE LIMITED ,  IMMUNOCORE LIMITED

Inventor： Bent Karsten Jakobsen ,  Nathaniel Ross Liddy ,  Peter Eamon Molloy ,  Annelise Brigitte Vuidepot

IPC: C40B40/10 ,  C07K14/725 ,  C12N15/10 ,  C40B40/02

Abstract: The present invention relates to a library of particles, the library displaying a plurality of different T cell receptors (TCRs), wherein the plurality of TCRs consists essentially of TCRs comprising an alpha chain variable domain and a beta chain variable domain, wherein the alpha chain variable domain comprises a TRAV12-2 gene product and the beta chain variable domain comprises a TRBV gene product.

公开(公告)号：US20210261948A1

公开(公告)日：2021-08-26

申请号：US17316381

申请日：2021-05-10

Applicant: NantBio, Inc.

Inventor： Clifford Anders Olson ,  Kayvan Niazi

IPC: C12N15/10 ,  C40B40/10 ,  C12N15/86 ,  C12N5/09 ,  C40B40/02 ,  C40B50/06

Abstract: Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

公开(公告)号：US11097010B2

公开(公告)日：2021-08-24

申请号：US16323727

申请日：2017-08-04

Applicant: Ossianix, Inc.

Inventor： Pawel Stocki ,  Krzysztof Bartlomiej Wicher ,  Julia Lynn Rutkowski ,  Fabrizio Comper ,  Mykhaylo Demydchuk ,  Jaroslaw Michal Szary

IPC: A61K47/64 ,  G01N33/68 ,  C07K16/28 ,  C07K16/40 ,  C12N15/10 ,  C12N15/09 ,  G01N33/58 ,  A61K47/56 ,  C12N15/113 ,  A61K39/00 ,  C40B30/06 ,  C40B40/10

Abstract: The present invention relates to the fields of molecular medicine and targeted delivery of therapeutic or diagnostic agents to cells outside the vascular system and into the parenchymal tissue of organs within the body. More specifically, the present invention relates to the methods used to identify membrane receptors or transporters capable of carrying cargo specifically targeted to the parenchymal tissue of the brain and to in vivo enrichment methods for selecting peptides that are transported across the blood-brain barrier (“BBB”), or analogously, across other membrane containing organs or structures, such as liver, spleen, kidney and tumors.